3 Bio-microelectromechanical Systems (BioMEMS) in Bio-sensing …
75
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
Lab-on-chip
• Monolith column
• LIF system
• Inlets
Hot embossing was
used to transfer
channel features into
the propylene
microdevices with the
help of CNC
aluminum masters,
while the holes were
drilled for inlets and
outlets. The monoliths
were formed by a
single-step method by
filling a device with
polymerization
mixture and photo
initiator and exposing
to UV light
A lysate sample was
directed through the
column monolith. The
porous allowed
selectivity to the
target DNA. After a
rinse step, captured
DNA was released by
heating the monolith
above the melting
point of the DNA
dsDNA from the
carbapenemase gene
The lowest detected
concentration of
KPC amplicons
was 100 pM using
this LIF system.
The capture
efficiency of the
target was 86%,
while its labelling
was done with 97%
accuracy
Knob et al.
(2018)
(continued)