2 Bio-microelectromechanical Systems (BioMEMS) …
35
Table 2.1
(continued)
BioMEMS platform
Main components
Fabrication strategy Mechanisms of
operation
Detected analyte
Specifics
References
Electronic-based
ELISA
• Circuit board
• TFT touch screen
• Au surface
• Commercially
available IFNγ
Using heat and
adhesive properties,
Cu foil was
laminated on FR4
PCB substrate and a
gold layer was plated
on top. The PMMA
wells were fixed on
the PCB surface
TMB was used as the
reporter reagent.
Amperometric
detection was
performed by a second
generation
amperometry
• IFNγ
This device
facilitated
colorimetric and
amperometric
assays in an
inexpensive and
portable manner
Evans
(2017)
Centrifugal
microfluidic device
integrated with LAMP
• PMMA layers
• PSA layer
• Loading and mixing
chambers
• Smartphone
The device was made
of two PMMA layers
and a middle PSA
layer press-bounded
together. A
square-wave
microchannel,
metering chambers,
and RPM control
allowed the process
to flow
The LAMP reagents
and primers were
injected to the loading
chambers while the
sealing material was
loaded into the sealing
chambers and the wax
into the wax valve and
the DNA samples were
injected into the
amplification
chambers
• E. coli
• Vibrio cholerae
This device allowed
30 simultaneous
genetic analyses of
three different
foodborne
pathogens. This
process was
performed in
approx. an hour and
presented a
considerably low
LOD
Sayad et al.
(2017)
(continued)
35
Table 2.1
(continued)
BioMEMS platform
Main components
Fabrication strategy Mechanisms of
operation
Detected analyte
Specifics
References
Electronic-based
ELISA
• Circuit board
• TFT touch screen
• Au surface
• Commercially
available IFNγ
Using heat and
adhesive properties,
Cu foil was
laminated on FR4
PCB substrate and a
gold layer was plated
on top. The PMMA
wells were fixed on
the PCB surface
TMB was used as the
reporter reagent.
Amperometric
detection was
performed by a second
generation
amperometry
• IFNγ
This device
facilitated
colorimetric and
amperometric
assays in an
inexpensive and
portable manner
Evans
(2017)
Centrifugal
microfluidic device
integrated with LAMP
• PMMA layers
• PSA layer
• Loading and mixing
chambers
• Smartphone
The device was made
of two PMMA layers
and a middle PSA
layer press-bounded
together. A
square-wave
microchannel,
metering chambers,
and RPM control
allowed the process
to flow
The LAMP reagents
and primers were
injected to the loading
chambers while the
sealing material was
loaded into the sealing
chambers and the wax
into the wax valve and
the DNA samples were
injected into the
amplification
chambers
• E. coli
• Vibrio cholerae
This device allowed
30 simultaneous
genetic analyses of
three different
foodborne
pathogens. This
process was
performed in
approx. an hour and
presented a
considerably low
LOD
Sayad et al.
(2017)
(continued)