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A. S. Cerda-Kipper and S. Hosseini
7.2 Biochemiluminescence Detection Strategy
The first BL-CL based biosensors used an analyte-specific enzyme coupled with one
or more “indicating” enzymes resulting in BL or CL emission (Wild 2013). One of the
main advantages of BL-CL is its high detectability of the luminescence signal through
high quantum efficiency (Roda et al. 2004). However, the main limitation of BL-CLbased biosensors is the different pH requirements for the enzyme reactions (Wild
2013; Microfluidic chip for biological chemiluminescence detection and detection
method thereof 2012). In this chapter, we present the latest strategies reported for the
development of BioMEMS that operate based on the BL-CL principle and are aimed
at specific an application: biosensing.
7.3 Recent Advances of Biochemiluminescence Detection
in Microfluidics BioMEMS
7.3.1 Recent Advances of Biochemiluminescence Detection
in Lab-On-Chip (LOC) Devices
A patent describing a microfluidic chip for BL-CL detection was disclosed from
China, to measure components inside human single blood erythrocyte (Microfluidic
chip for biological chemiluminescence detection and detection method thereof 2012).
The chip comprised of an interface layer, a transparent layer, a channel, a reflecting
layer and a fixed layer, which were sequentially arranged from top to bottom of
the device fixed by fasteners. The layers were connected by adhesives and fixed by
fasteners. The interface layer and the transparent layer were provided with a liquid
inlet and an outlet hole, respectively. The interface layer was comprised of an optical
fiber interface. The channel layer had a liquid inlet flow channel, a micro mixer, a
detecting pool, and a waste liquid buffer pool, which have sequentially communicated
with each other. The optical fiber interface was arranged above the detecting pool,
while the bottom of the optical fiber interface was connected with a light penetration
layer. The detecting pool was in contact with the lower surface of the channel layer
and the bottom of the detecting pool was connected to the reflecting layer. Moreover,
the bottom of the waste liquid buffer pool was connected to the fixed layer. The
fluorescence micro-spectrum detection was performed in a single step flow passage
structure, thus improving luminous intensity by enhancing solution mixability, and
optical/light detection efficiency of BL-CL detection. This microfluidic chip has
shown the advantages of simple fabrication method, convenient operation, rapid
detection, high sensitivity, and accurate detection result, as described in Table 7.1.
Another patent from Japan used cartridge for measurement of BL-CL (Measuring
method, cartridge for measurement, and measuring device 2013). The measurement
took place through injecting a sample into a reaction tank via an inlet side in the flow
A. S. Cerda-Kipper and S. Hosseini
7.2 Biochemiluminescence Detection Strategy
The first BL-CL based biosensors used an analyte-specific enzyme coupled with one
or more “indicating” enzymes resulting in BL or CL emission (Wild 2013). One of the
main advantages of BL-CL is its high detectability of the luminescence signal through
high quantum efficiency (Roda et al. 2004). However, the main limitation of BL-CLbased biosensors is the different pH requirements for the enzyme reactions (Wild
2013; Microfluidic chip for biological chemiluminescence detection and detection
method thereof 2012). In this chapter, we present the latest strategies reported for the
development of BioMEMS that operate based on the BL-CL principle and are aimed
at specific an application: biosensing.
7.3 Recent Advances of Biochemiluminescence Detection
in Microfluidics BioMEMS
7.3.1 Recent Advances of Biochemiluminescence Detection
in Lab-On-Chip (LOC) Devices
A patent describing a microfluidic chip for BL-CL detection was disclosed from
China, to measure components inside human single blood erythrocyte (Microfluidic
chip for biological chemiluminescence detection and detection method thereof 2012).
The chip comprised of an interface layer, a transparent layer, a channel, a reflecting
layer and a fixed layer, which were sequentially arranged from top to bottom of
the device fixed by fasteners. The layers were connected by adhesives and fixed by
fasteners. The interface layer and the transparent layer were provided with a liquid
inlet and an outlet hole, respectively. The interface layer was comprised of an optical
fiber interface. The channel layer had a liquid inlet flow channel, a micro mixer, a
detecting pool, and a waste liquid buffer pool, which have sequentially communicated
with each other. The optical fiber interface was arranged above the detecting pool,
while the bottom of the optical fiber interface was connected with a light penetration
layer. The detecting pool was in contact with the lower surface of the channel layer
and the bottom of the detecting pool was connected to the reflecting layer. Moreover,
the bottom of the waste liquid buffer pool was connected to the fixed layer. The
fluorescence micro-spectrum detection was performed in a single step flow passage
structure, thus improving luminous intensity by enhancing solution mixability, and
optical/light detection efficiency of BL-CL detection. This microfluidic chip has
shown the advantages of simple fabrication method, convenient operation, rapid
detection, high sensitivity, and accurate detection result, as described in Table 7.1.
Another patent from Japan used cartridge for measurement of BL-CL (Measuring
method, cartridge for measurement, and measuring device 2013). The measurement
took place through injecting a sample into a reaction tank via an inlet side in the flow