36
S. Sowmiya Sri et al.
pure-cultures sourced deep work has been published on the degradation of pesticide,
there is a dearth of understanding on the impact; these xenobiotic-extreme environments on the indigenous microbes augmented and attenuated due these contaminations. Since culture-based approach expose only less than 1% of the total genome,
metagenomic profiling is mandatory to expose the entire microbial diversity within
contaminated sites, thus the need and role of biotechnological techniques. Hence
work highlighting the impact of long-term pesticide exposure on the diversity of
microbial communities are available only on a miniscule scale (Regar et al. 2019).
For example, an extended heavy metal contaminated site in China was scrutinized
to understand the role and function of microbial communities. Many taxa including
Proteobacteria, Bacteroidetes, and Firmicutes illuminated via this study had the resistance against assorted heavy metals (Chen et al. 2018). Other studies conducted at
Tibetan (steppe) exposed that the soil parameters and minerals had an impact on the
microbial diversity and its attributes (Zhou et al. 2019). Procurement of the entire
microbiome especially in polluted soil has gained traction in recent years and this
study is one such attempt to glen potential and prospective microorganisms from
pesticide contaminated soil.
2 Materials and Methods
2.1 Collection of Samples
A soil sample was collected from the polluted site. The collected sample was kept
in a sterile container, placed in a sterile bag, transferred to the laboratory and stored
at 4 °C until further use. The sample was taken as 1 g and ground using mortar and
pestle into small pieces and further proceeded to serial dilution. After serial dilution
technique, the sample was introduced into the nutrient broth for growth of the culture
(Choi et al. 2015).
2.2 Isolation of Bacteria
Isolating bacteria from the soil is an important step, once they are isolated, bacteria
can be further analysed to determine things, such as their species and their function in
the soil environment (Sayyadifar et al. 2012). The bacteria are isolated using specific
media like starch medium and Skim milk agar medium (Table 1).
S. Sowmiya Sri et al.
pure-cultures sourced deep work has been published on the degradation of pesticide,
there is a dearth of understanding on the impact; these xenobiotic-extreme environments on the indigenous microbes augmented and attenuated due these contaminations. Since culture-based approach expose only less than 1% of the total genome,
metagenomic profiling is mandatory to expose the entire microbial diversity within
contaminated sites, thus the need and role of biotechnological techniques. Hence
work highlighting the impact of long-term pesticide exposure on the diversity of
microbial communities are available only on a miniscule scale (Regar et al. 2019).
For example, an extended heavy metal contaminated site in China was scrutinized
to understand the role and function of microbial communities. Many taxa including
Proteobacteria, Bacteroidetes, and Firmicutes illuminated via this study had the resistance against assorted heavy metals (Chen et al. 2018). Other studies conducted at
Tibetan (steppe) exposed that the soil parameters and minerals had an impact on the
microbial diversity and its attributes (Zhou et al. 2019). Procurement of the entire
microbiome especially in polluted soil has gained traction in recent years and this
study is one such attempt to glen potential and prospective microorganisms from
pesticide contaminated soil.
2 Materials and Methods
2.1 Collection of Samples
A soil sample was collected from the polluted site. The collected sample was kept
in a sterile container, placed in a sterile bag, transferred to the laboratory and stored
at 4 °C until further use. The sample was taken as 1 g and ground using mortar and
pestle into small pieces and further proceeded to serial dilution. After serial dilution
technique, the sample was introduced into the nutrient broth for growth of the culture
(Choi et al. 2015).
2.2 Isolation of Bacteria
Isolating bacteria from the soil is an important step, once they are isolated, bacteria
can be further analysed to determine things, such as their species and their function in
the soil environment (Sayyadifar et al. 2012). The bacteria are isolated using specific
media like starch medium and Skim milk agar medium (Table 1).