Microbiome Analysis of Pesticide Contaminated Soils Its Impact …
37
Table 1 Results from
isolation media
S. No.
Media used
Result
1
Skim milk
Enterobacter cloacae isolated
2
Starch
No microorganism
3 Result and Discussions
3.1 DNA Extraction and PCR Sequencing of Bacterial
Sample
The isolated foodborne pathogens were processed to identify the bacteria using
molecular biology techniques. The steps to be followed for the extraction of DNA
from the bacterial sample in the laboratories are shown in the Fig. 1. Bacterial
Genomic DNA Spin-50 kit was used. The solution was prepared with the necessary components to make the RNase solution. About 100 mL of bacterial culture
was taken and added with the required components. The DNA concentration was
determined by both UV spectrophotometer and quantitative analysis on Agarose
gel. Finally, sequencing was obtained with the aid of the ABI sequencing machine
(Babalola 2003).
3.2 Enterobacter Cloacae
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs
in water, sewage, soil, meat, hospital environments, and on the skin and the intestinal
tract of man and animals as a commensal. Enterobacter cloacae is a facultative anaerobic bacterium and are considered to be an important cause of nosocomial infection.
However, the isolated E. cloacae showed higher furfural-tolerant cellular growth,
compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the genome sequence of E. cloacae isolated
from polluted soil. The genomic DNA sequence of E. cloacae will provide valuable genetic resources for engineering of industrially relevant strains (Shankar et al.
2012).
3.3 Phylogenetic Tree
Phylogenetic study was performed to map the molecular level similarity of DNA
sequence among the various species. Gene sequence of Enterobacter Cloacae was
taken for the study. This study has been performed to enlarge the molecular level
gene similarly identification to enhance the various Enterobacter species (Gong et al.
2015).
37
Table 1 Results from
isolation media
S. No.
Media used
Result
1
Skim milk
Enterobacter cloacae isolated
2
Starch
No microorganism
3 Result and Discussions
3.1 DNA Extraction and PCR Sequencing of Bacterial
Sample
The isolated foodborne pathogens were processed to identify the bacteria using
molecular biology techniques. The steps to be followed for the extraction of DNA
from the bacterial sample in the laboratories are shown in the Fig. 1. Bacterial
Genomic DNA Spin-50 kit was used. The solution was prepared with the necessary components to make the RNase solution. About 100 mL of bacterial culture
was taken and added with the required components. The DNA concentration was
determined by both UV spectrophotometer and quantitative analysis on Agarose
gel. Finally, sequencing was obtained with the aid of the ABI sequencing machine
(Babalola 2003).
3.2 Enterobacter Cloacae
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs
in water, sewage, soil, meat, hospital environments, and on the skin and the intestinal
tract of man and animals as a commensal. Enterobacter cloacae is a facultative anaerobic bacterium and are considered to be an important cause of nosocomial infection.
However, the isolated E. cloacae showed higher furfural-tolerant cellular growth,
compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the genome sequence of E. cloacae isolated
from polluted soil. The genomic DNA sequence of E. cloacae will provide valuable genetic resources for engineering of industrially relevant strains (Shankar et al.
2012).
3.3 Phylogenetic Tree
Phylogenetic study was performed to map the molecular level similarity of DNA
sequence among the various species. Gene sequence of Enterobacter Cloacae was
taken for the study. This study has been performed to enlarge the molecular level
gene similarly identification to enhance the various Enterobacter species (Gong et al.
2015).