Microbiome Analysis of Pesticide
Contaminated Soils Its Impact
on the Microbial Isolates
S. Sowmiya Sri, V. Suji, and G. Sophia Reena
Abstract The study aims to analyse the bacterial pattern, to isolate the bacteria
from polluted soil site and to identify the bacteria using 16s rDNA gene sequencing.
The present research is carried out to isolate and identify the bacteria using molecular study. The bacteria is isolated using plating agar. The DNA extracted and PCR
carried out. Further, the gene sequence was analyzed and the sequence was submitted
to NCBI and accession number was allotted as MT126476. The bacteria identified as Enterobacter cloacae, the current interest in using omics technologies for
elucidating the microbial diversity from potential contaminated site for harnessing
microorganism for potential bioremediation perspectives.
1 Introduction
Soil bacteria from micro-aggregate in the soil acts as a binder for soil particles with
their secretions. Each of these micro-organisms groups has its characteristics that
will define their functions in the soil. Bacteria are most significant in ecosystems
in the soil including improved soil structure and soil aggregation, reprocess of soil
nutrients. Soil is rich in microorganisms which are capable of producing commercially viable metabolites. The traditional approach is random screening in which
bacteria are isolated, grown and their activity spectrum was assessed. Soil bacteria
are very important in biogeochemical cycles and have been used for crop for decades
(Zhao et al. 2018). The numbers and species of microbes in soil depend on environmental factors like nutrient availability, soil texture, presence of moisture content in
soil and type of vegetation cover, and their number changes according to the type of
environmental factors (Liu et al. 2012).
Contamination of soils with an array of pesticides, due to the widespread application of these pesticide in the ecosystem, their comprehensive toxicological effect
on human health risk assessment and ecotoxicological implications. Although many
S. Sowmiya Sri · V. Suji · G. Sophia Reena (B)
Department of Information Technology, PSGR Krishnammal College for Women, Coimbatore,
Tamil Nadu 641004, India
e-mail: sophiareena@psgrkcw.ac.in
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
M. Prashanthi et al. (eds.), Bioremediation and Green Technologies, Environmental
Science and Engineering, https://doi.org/10.1007/978-3-030-64122-1_3
35
Contaminated Soils Its Impact
on the Microbial Isolates
S. Sowmiya Sri, V. Suji, and G. Sophia Reena
Abstract The study aims to analyse the bacterial pattern, to isolate the bacteria
from polluted soil site and to identify the bacteria using 16s rDNA gene sequencing.
The present research is carried out to isolate and identify the bacteria using molecular study. The bacteria is isolated using plating agar. The DNA extracted and PCR
carried out. Further, the gene sequence was analyzed and the sequence was submitted
to NCBI and accession number was allotted as MT126476. The bacteria identified as Enterobacter cloacae, the current interest in using omics technologies for
elucidating the microbial diversity from potential contaminated site for harnessing
microorganism for potential bioremediation perspectives.
1 Introduction
Soil bacteria from micro-aggregate in the soil acts as a binder for soil particles with
their secretions. Each of these micro-organisms groups has its characteristics that
will define their functions in the soil. Bacteria are most significant in ecosystems
in the soil including improved soil structure and soil aggregation, reprocess of soil
nutrients. Soil is rich in microorganisms which are capable of producing commercially viable metabolites. The traditional approach is random screening in which
bacteria are isolated, grown and their activity spectrum was assessed. Soil bacteria
are very important in biogeochemical cycles and have been used for crop for decades
(Zhao et al. 2018). The numbers and species of microbes in soil depend on environmental factors like nutrient availability, soil texture, presence of moisture content in
soil and type of vegetation cover, and their number changes according to the type of
environmental factors (Liu et al. 2012).
Contamination of soils with an array of pesticides, due to the widespread application of these pesticide in the ecosystem, their comprehensive toxicological effect
on human health risk assessment and ecotoxicological implications. Although many
S. Sowmiya Sri · V. Suji · G. Sophia Reena (B)
Department of Information Technology, PSGR Krishnammal College for Women, Coimbatore,
Tamil Nadu 641004, India
e-mail: sophiareena@psgrkcw.ac.in
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2021
M. Prashanthi et al. (eds.), Bioremediation and Green Technologies, Environmental
Science and Engineering, https://doi.org/10.1007/978-3-030-64122-1_3
35