of ethanol metabolism in Saccharomyces cerevisiae
by ATP-independent acetylating acetaldehyde
dehydrogenase. FEMS Yeast Res 16:fow006
Krivoruchko A, Serrano-Amatriain C, Chen Y, Siewers
V, Nielsen J (2013) Improving biobutanol production in engineered Saccharomyces cerevisiae by
manipulation of acetyl-CoA metabolism. J Ind
Microbiol Biotechnol 40:1051–1056
Krivoruchko A, Zhang Y, Siewers V, Chen Y, Nielsen J
(2015) Microbial acetyl-CoA metabolism and metabolic engineering. Metab Eng 28:28–42
Kruis AJ, Gallone B, Jonker T, Mars AE, van Rijswijck
IMH, JCM W-R, Smid EJ, Steensels J, Verstrepen
KJ, SWM K, van der Oost J, Weusthuis RA (2018)
Contribution of Eat1 and other alcohol acyltransferases to ester production in Saccharomyces cerevisiae. Front Microbiol 9:345
Lafon-Lafourcade S, Geneix C, Ribe ´reau-Gayon P
(1984) Inhibition of alcoholic fermentation of
grape must by fatty acids produced by yeasts and
their elimination by yeast ghosts. Appl Environ
Microbiol 47:1246–1249
Leber C, Da Silva NA (2014) Engineering of Saccharomyces cerevisiae for the synthesis of short chain
fatty acids. Biotechnol Bioeng 111:347–358
Leber C, Polson B, Fernandez-Moya R, Da Silva NA
(2015) Overproduction and secretion of free fatty
acids through disrupted neutral lipid recycle in
Saccharomyces cerevisiae. Metab Eng 28:54–62
Leber C, Choi JW, Polson B, Da Silva NA (2016) Disrupted short chain specific b-oxidation and
improved synthase expression increase synthesis
of short chain fatty acids in Saccharomyces cerevisiae. Biotechnol Bioeng 113:895–900
Lee ME, DeLoache WC, Cervantes B, Dueber JE (2015)
A highly characterized yeast toolkit for modular,
multipart assembly. ACS Synth Biol 4:975–986
Legras JL, Erny C, Le Jeune C, Lollier M, Adolphe Y,
Demuyter C, Delobel P, Blondin B, Karst F (2010)
Activation of two different resistance mechanisms
in Saccharomyces cerevisiae upon exposure to
octanoic and decanoic acids. Appl Environ Microbiol 76:7526–7535
Leibundgut M, Maier T, Jenni S, Ban N (2008) The
multienzyme architecture of eukaryotic fatty acid
synthases. Curr Opin Struct Biol 18:714–725
Lennen RM, Pfleger BF (2013) Microbial production of
fatty acid-derived fuels and chemicals. Curr Opin
Biotechnol 24:1044–1053
Li X, Guo D, Cheng Y, Zhu F, Deng Z, Liu T (2014)
Overproduction of fatty acids in engineered Saccharomyces
cerevisiae.
Biotechnol
Bioeng
111:1841–1852
Li S, Si T, Wang M, Zhao H (2015) Development
of a synthetic malonyl-CoA sensor in Saccharomyces cerevisiae for intracellular metabolite monitoring and genetic screening. ACS Synth Biol
4:1308–1315
Lian J, Zhao H (2015) Reversal of the b-oxidation cycle
in Saccharomyces cerevisiae for production of fuels
and chemicals. ACS Synth Biol 4:332–341
Lian J, Si T, Nair NU, Zhao H (2014) Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. Metab Eng 24:139–149
Ling H, Chen B, Kang A, Lee J-M, Chang M (2013)
Transcriptome response to alkane biofuels in Saccharomyces cerevisiae: identification of efflux
pumps involved in alkane tolerance. Biotechnol
Biofuels 6:95
Liu H, Liu K, Yan M, Xu L, Ouyang P (2011) gTME for
improved adaptation of Saccharomyces cerevisiae
to corn cob acid hydrolysate. Appl Biochem Biotechnol 164:1150–1159
Liu P, Chernyshov A, Najdi T, Fu Y, Dickerson J, Sandmeyer S, Jarboe L (2013) Membrane stress caused
by octanoic acid in Saccharomyces cerevisiae. Appl
Microbiol Biotechnol 97:3239–3251
Liu W, Zhang B, Jiang R (2017) Improving acetyl-CoA
biosynthesis in Saccharomyces cerevisiae via the
overexpression of pantothenate kinase and PDH
bypass. Biotechnol Biofuels 10:97
Lomakin IB, Xiong Y, Steitz TA (2007) The crystal
structure of yeast fatty acid synthase, a cellular
machine with eight active sites working together.
Cell 129:319–332
Mans R, Daran J-MG, Pronk JT (2018) Under pressure:
evolutionary engineering of yeast strains for
improved performance in fuels and chemicals production. Curr Opin Biotechnol 50:47–56
Marella ER, Holkenbrink C, Siewers V, Borodina I
(2018) Engineering microbial fatty acid metabolism for biofuels and biochemicals. Curr Opin Biotechnol 50:39–46
Meadows AL, Hawkins KM, Tsegaye Y, Antipov E, Kim
Y, Raetz L, Dahl RH, Tai A, Mahatdejkul-Meadows
T, Xu L, Zhao L, Dasika MS, Murarka A, Lenihan J,
Eng D, Leng JS, Liu C-L, Wenger JW, Jiang H, Chao
L, Westfall P, Lai J, Ganesan S, Jackson P, Mans R,
Platt D, Reeves CD, Saija PR, Wichmann G,
Holmes VF, Benjamin K, Hill PW, Gardner TS,
Tsong AE (2016) Rewriting yeast central carbon
metabolism for industrial isoprenoid production.
Nature 537:694–697
Membrillo-Hernandez J, Echave P, Cabiscol E, Tamarit
J, Ros J, Lin EC (2000) Evolution of the adhE gene
product of Escherichia coli from a functional
reductase to a dehydrogenase. Genetic and biochemical studies of the mutant proteins. J Biol
Chem 275:33869–33875
Michener JK, Thodey K, Liang JC, Smolke CD (2012)
Applications of genetically-encoded biosensors for
the construction and control of biosynthetic pathways. Metab Eng 14:212–222
Mukherjee K, Bhattacharyya S, Peralta-Yahya P (2015)
GPCR-based chemical biosensors for mediumchain fatty acids. ACS Synth Biol 4:1261–1269
364
L. Baumann et al.
by ATP-independent acetylating acetaldehyde
dehydrogenase. FEMS Yeast Res 16:fow006
Krivoruchko A, Serrano-Amatriain C, Chen Y, Siewers
V, Nielsen J (2013) Improving biobutanol production in engineered Saccharomyces cerevisiae by
manipulation of acetyl-CoA metabolism. J Ind
Microbiol Biotechnol 40:1051–1056
Krivoruchko A, Zhang Y, Siewers V, Chen Y, Nielsen J
(2015) Microbial acetyl-CoA metabolism and metabolic engineering. Metab Eng 28:28–42
Kruis AJ, Gallone B, Jonker T, Mars AE, van Rijswijck
IMH, JCM W-R, Smid EJ, Steensels J, Verstrepen
KJ, SWM K, van der Oost J, Weusthuis RA (2018)
Contribution of Eat1 and other alcohol acyltransferases to ester production in Saccharomyces cerevisiae. Front Microbiol 9:345
Lafon-Lafourcade S, Geneix C, Ribe ´reau-Gayon P
(1984) Inhibition of alcoholic fermentation of
grape must by fatty acids produced by yeasts and
their elimination by yeast ghosts. Appl Environ
Microbiol 47:1246–1249
Leber C, Da Silva NA (2014) Engineering of Saccharomyces cerevisiae for the synthesis of short chain
fatty acids. Biotechnol Bioeng 111:347–358
Leber C, Polson B, Fernandez-Moya R, Da Silva NA
(2015) Overproduction and secretion of free fatty
acids through disrupted neutral lipid recycle in
Saccharomyces cerevisiae. Metab Eng 28:54–62
Leber C, Choi JW, Polson B, Da Silva NA (2016) Disrupted short chain specific b-oxidation and
improved synthase expression increase synthesis
of short chain fatty acids in Saccharomyces cerevisiae. Biotechnol Bioeng 113:895–900
Lee ME, DeLoache WC, Cervantes B, Dueber JE (2015)
A highly characterized yeast toolkit for modular,
multipart assembly. ACS Synth Biol 4:975–986
Legras JL, Erny C, Le Jeune C, Lollier M, Adolphe Y,
Demuyter C, Delobel P, Blondin B, Karst F (2010)
Activation of two different resistance mechanisms
in Saccharomyces cerevisiae upon exposure to
octanoic and decanoic acids. Appl Environ Microbiol 76:7526–7535
Leibundgut M, Maier T, Jenni S, Ban N (2008) The
multienzyme architecture of eukaryotic fatty acid
synthases. Curr Opin Struct Biol 18:714–725
Lennen RM, Pfleger BF (2013) Microbial production of
fatty acid-derived fuels and chemicals. Curr Opin
Biotechnol 24:1044–1053
Li X, Guo D, Cheng Y, Zhu F, Deng Z, Liu T (2014)
Overproduction of fatty acids in engineered Saccharomyces
cerevisiae.
Biotechnol
Bioeng
111:1841–1852
Li S, Si T, Wang M, Zhao H (2015) Development
of a synthetic malonyl-CoA sensor in Saccharomyces cerevisiae for intracellular metabolite monitoring and genetic screening. ACS Synth Biol
4:1308–1315
Lian J, Zhao H (2015) Reversal of the b-oxidation cycle
in Saccharomyces cerevisiae for production of fuels
and chemicals. ACS Synth Biol 4:332–341
Lian J, Si T, Nair NU, Zhao H (2014) Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains. Metab Eng 24:139–149
Ling H, Chen B, Kang A, Lee J-M, Chang M (2013)
Transcriptome response to alkane biofuels in Saccharomyces cerevisiae: identification of efflux
pumps involved in alkane tolerance. Biotechnol
Biofuels 6:95
Liu H, Liu K, Yan M, Xu L, Ouyang P (2011) gTME for
improved adaptation of Saccharomyces cerevisiae
to corn cob acid hydrolysate. Appl Biochem Biotechnol 164:1150–1159
Liu P, Chernyshov A, Najdi T, Fu Y, Dickerson J, Sandmeyer S, Jarboe L (2013) Membrane stress caused
by octanoic acid in Saccharomyces cerevisiae. Appl
Microbiol Biotechnol 97:3239–3251
Liu W, Zhang B, Jiang R (2017) Improving acetyl-CoA
biosynthesis in Saccharomyces cerevisiae via the
overexpression of pantothenate kinase and PDH
bypass. Biotechnol Biofuels 10:97
Lomakin IB, Xiong Y, Steitz TA (2007) The crystal
structure of yeast fatty acid synthase, a cellular
machine with eight active sites working together.
Cell 129:319–332
Mans R, Daran J-MG, Pronk JT (2018) Under pressure:
evolutionary engineering of yeast strains for
improved performance in fuels and chemicals production. Curr Opin Biotechnol 50:47–56
Marella ER, Holkenbrink C, Siewers V, Borodina I
(2018) Engineering microbial fatty acid metabolism for biofuels and biochemicals. Curr Opin Biotechnol 50:39–46
Meadows AL, Hawkins KM, Tsegaye Y, Antipov E, Kim
Y, Raetz L, Dahl RH, Tai A, Mahatdejkul-Meadows
T, Xu L, Zhao L, Dasika MS, Murarka A, Lenihan J,
Eng D, Leng JS, Liu C-L, Wenger JW, Jiang H, Chao
L, Westfall P, Lai J, Ganesan S, Jackson P, Mans R,
Platt D, Reeves CD, Saija PR, Wichmann G,
Holmes VF, Benjamin K, Hill PW, Gardner TS,
Tsong AE (2016) Rewriting yeast central carbon
metabolism for industrial isoprenoid production.
Nature 537:694–697
Membrillo-Hernandez J, Echave P, Cabiscol E, Tamarit
J, Ros J, Lin EC (2000) Evolution of the adhE gene
product of Escherichia coli from a functional
reductase to a dehydrogenase. Genetic and biochemical studies of the mutant proteins. J Biol
Chem 275:33869–33875
Michener JK, Thodey K, Liang JC, Smolke CD (2012)
Applications of genetically-encoded biosensors for
the construction and control of biosynthetic pathways. Metab Eng 14:212–222
Mukherjee K, Bhattacharyya S, Peralta-Yahya P (2015)
GPCR-based chemical biosensors for mediumchain fatty acids. ACS Synth Biol 4:1261–1269
364
L. Baumann et al.