cerevisiae strains for optimum growth. FEMS
Yeast Res 12:796–808
Henritzi S, Fischer M, Grininger M, Oreb M, Boles E
(2018) An engineered fatty acid synthase combined with a carboxylic acid reductase enables de
novo production of 1-octanol in Saccharomyces
cerevisiae. Biotechnol Biofuels 11:150
Hettema EH, Tabak HF (2000) Transport of fatty acids
and metabolites across the peroxisomal membrane. Biochim Biophys Acta 1486:18–27
Hettema EH, van Roermund CW, Distel B, van den Berg
M, Vilela C, Rodrigues-Pousada C, Wanders RJ,
Tabak HF (1996) The ABC transporter proteins
Pat1 and Pat2 are required for import of longchain fatty acids into peroxisomes of Saccharomyces cerevisiae. EMBO J 15:3813–3822
Hiltunen JK, Mursula AM, Rottensteiner H, Wierenga
RK, Kastaniotis AJ, Gurvitz A (2003) The biochemistry of peroxisomal b-oxidation in the yeast
Saccharomyces cerevisiae. FEMS Microbiol Rev
27:35–64
Hirasawa T, Yoshikawa K, Nakakura Y, Nagahisa K,
Furusawa C, Katakura Y, Shimizu H, Shioya S
(2007) Identification of target genes conferring
ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis. J
Biotechnol 131:34–44
Hofbauer HF, Schopf FH, Schleifer H, Knittelfelder OL,
Pieber B, Rechberger GN, Wolinski H, Gaspar ML,
Kappe CO, Stadlmann J, Mechtler K, Zenz A,
Lohner K, Tehlivets O, Henry SA, Kohlwein SD
(2014) Regulation of gene expression through a
transcriptional repressor that senses acyl-chain
length in membrane phospholipids. Dev Cell
29:729–739
Hu Y, Zhu Z, Nielsen J, Siewers V (2018) Heterologous
transporter expression for improved fatty alcohol
secretion in yeast. Metab Eng 45:51–58
Jin Z, Wong A, Foo JL, Ng J, Cao Y-X, Chang MW, Yuan
Y-J (2016) Engineering Saccharomyces cerevisiae
to produce odd chain-length fatty alcohols. Biotechnol Bioeng 113:842–851
Johnson DR, Knoll LJ, Rowleyo N, Gordon JI (1994)
Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating
protein N-myristoylation. J Biol Chem 269
(27):18037–18046.
http://www.jbc.org/content/
269/27/18037.full.pdf
Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbu ¨chel A, Tee KL, Xu P, Wong TS (2017) Design
and application of genetically-encoded malonylCoA biosensors for metabolic engineering of
microbial cell factories. Metab Eng 44:253–264
Kamisaka Y, Tomita N, Kimura K, Kainou K, Uemura H
(2007) DGA1 (diacylglycerol acyltransferase gene)
overexpression and leucine biosynthesis significantly increase lipid accumulation in the Dsnf2
disruptant of Saccharomyces cerevisiae. Biochem
J 408:61–68
Kamisaka Y, Kimura K, Uemura H, Yamaoka M (2015)
Addition of methionine and low cultivation temperatures increase palmitoleic acid production by
engineered Saccharomyces cerevisiae. Appl Microbiol Biotechnol 99:201–210
Kaneda K, Imaizumi S, Yano I (1995) Distribution of
C22-, C24- and C26-a-unit-containing mycolic
acid homologues in mycobacteria. Microbiol
Immunol 39:563–570
Kang M-K, Zhou YJ, Buijs NA, Nielsen J (2017) Functional screening of aldehyde decarbonylases for
long-chain alkane production by Saccharomyces
cerevisiae. Microb Cell Factories 16:320
Kim S, Gonzalez R (2018) Selective production of
decanoic acid from iterative reversal of b-oxidation pathway. Biotechnol Bioeng 115:1311–1320
Kim S, Clomburg JM, Gonzalez R (2015) Synthesis of
medium-chain length (C6-C10) fuels and chemicals via b-oxidation reversal in Escherichia coli. J
Ind Microbiol Biotechnol 42:465–475
Knappe J, Schacht J, Mo ¨ckel W, Ho ¨pner T, Vetter H,
Edenharder R (1969) Pyruvate formate-lyase reaction in Escherichia coli. The enzymatic system
converting an inactive form of the lyase into the
catalytically active enzyme. Eur J Biochem 11:316–
327
Knoll LJ, Johnson DR, Gordon JI (1994) Biochemical
studies of three Saccharomyces cerevisiae acyl-CoA
synthetases, Faalp, FaaZp, and Faa3p. J Biol Chem
269:16348–16356
Kocharin K, Chen Y, Siewers V, Nielsen J (2012) Engineering of acetyl-CoA metabolism for the
improved production of polyhydroxybutyrate in
Saccharomyces cerevisiae. AMB Express 2:52
Kocharin K, Siewers V, Nielsen J (2013) Improved polyhydroxybutyrate production by Saccharomyces
cerevisiae through the use of the phosphoketolase
pathway. Biotechnol Bioeng 110:2216–2224
Koopman F, Beekwilder J, Crimi B, van Houwelingen A,
Hall RD, Bosch D, van Maris AJA, Pronk JT, Daran
J-M (2012) De novo production of the flavonoid
naringenin in engineered Saccharomyces cerevisiae. Microb Cell Factories 11:155
Kozak BU, van Rossum HM, Luttik MAH, Akeroyd M,
Benjamin KR, Wu L, de Vries S, Daran J-M, Pronk
JT, van Maris AJA (2014a) Engineering acetyl
coenzyme A supply: functional expression of a
bacterial pyruvate dehydrogenase complex in the
cytosol of Saccharomyces cerevisiae. MBio 5:
e01696–e01614
Kozak BU, van Rossum HM, Benjamin KR, Wu L,
Daran J-MG, Pronk JT, van Maris AJA (2014b)
Replacement of the Saccharomyces cerevisiae
acetyl-CoA synthetases by alternative pathways
for cytosolic acetyl-CoA synthesis. Metab Eng
21:46–59
Kozak BU, van Rossum HM, Niemeijer MS, van Dijk M,
Benjamin K, Wu L, Daran J-MG, Pronk JT, van
Maris AJA (2016) Replacement of the initial steps
14 Engineering Saccharomyces cerevisiae for Production of Fatty Acids and Their Derivatives
363
Yeast Res 12:796–808
Henritzi S, Fischer M, Grininger M, Oreb M, Boles E
(2018) An engineered fatty acid synthase combined with a carboxylic acid reductase enables de
novo production of 1-octanol in Saccharomyces
cerevisiae. Biotechnol Biofuels 11:150
Hettema EH, Tabak HF (2000) Transport of fatty acids
and metabolites across the peroxisomal membrane. Biochim Biophys Acta 1486:18–27
Hettema EH, van Roermund CW, Distel B, van den Berg
M, Vilela C, Rodrigues-Pousada C, Wanders RJ,
Tabak HF (1996) The ABC transporter proteins
Pat1 and Pat2 are required for import of longchain fatty acids into peroxisomes of Saccharomyces cerevisiae. EMBO J 15:3813–3822
Hiltunen JK, Mursula AM, Rottensteiner H, Wierenga
RK, Kastaniotis AJ, Gurvitz A (2003) The biochemistry of peroxisomal b-oxidation in the yeast
Saccharomyces cerevisiae. FEMS Microbiol Rev
27:35–64
Hirasawa T, Yoshikawa K, Nakakura Y, Nagahisa K,
Furusawa C, Katakura Y, Shimizu H, Shioya S
(2007) Identification of target genes conferring
ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis. J
Biotechnol 131:34–44
Hofbauer HF, Schopf FH, Schleifer H, Knittelfelder OL,
Pieber B, Rechberger GN, Wolinski H, Gaspar ML,
Kappe CO, Stadlmann J, Mechtler K, Zenz A,
Lohner K, Tehlivets O, Henry SA, Kohlwein SD
(2014) Regulation of gene expression through a
transcriptional repressor that senses acyl-chain
length in membrane phospholipids. Dev Cell
29:729–739
Hu Y, Zhu Z, Nielsen J, Siewers V (2018) Heterologous
transporter expression for improved fatty alcohol
secretion in yeast. Metab Eng 45:51–58
Jin Z, Wong A, Foo JL, Ng J, Cao Y-X, Chang MW, Yuan
Y-J (2016) Engineering Saccharomyces cerevisiae
to produce odd chain-length fatty alcohols. Biotechnol Bioeng 113:842–851
Johnson DR, Knoll LJ, Rowleyo N, Gordon JI (1994)
Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating
protein N-myristoylation. J Biol Chem 269
(27):18037–18046.
http://www.jbc.org/content/
269/27/18037.full.pdf
Johnson AO, Gonzalez-Villanueva M, Wong L, Steinbu ¨chel A, Tee KL, Xu P, Wong TS (2017) Design
and application of genetically-encoded malonylCoA biosensors for metabolic engineering of
microbial cell factories. Metab Eng 44:253–264
Kamisaka Y, Tomita N, Kimura K, Kainou K, Uemura H
(2007) DGA1 (diacylglycerol acyltransferase gene)
overexpression and leucine biosynthesis significantly increase lipid accumulation in the Dsnf2
disruptant of Saccharomyces cerevisiae. Biochem
J 408:61–68
Kamisaka Y, Kimura K, Uemura H, Yamaoka M (2015)
Addition of methionine and low cultivation temperatures increase palmitoleic acid production by
engineered Saccharomyces cerevisiae. Appl Microbiol Biotechnol 99:201–210
Kaneda K, Imaizumi S, Yano I (1995) Distribution of
C22-, C24- and C26-a-unit-containing mycolic
acid homologues in mycobacteria. Microbiol
Immunol 39:563–570
Kang M-K, Zhou YJ, Buijs NA, Nielsen J (2017) Functional screening of aldehyde decarbonylases for
long-chain alkane production by Saccharomyces
cerevisiae. Microb Cell Factories 16:320
Kim S, Gonzalez R (2018) Selective production of
decanoic acid from iterative reversal of b-oxidation pathway. Biotechnol Bioeng 115:1311–1320
Kim S, Clomburg JM, Gonzalez R (2015) Synthesis of
medium-chain length (C6-C10) fuels and chemicals via b-oxidation reversal in Escherichia coli. J
Ind Microbiol Biotechnol 42:465–475
Knappe J, Schacht J, Mo ¨ckel W, Ho ¨pner T, Vetter H,
Edenharder R (1969) Pyruvate formate-lyase reaction in Escherichia coli. The enzymatic system
converting an inactive form of the lyase into the
catalytically active enzyme. Eur J Biochem 11:316–
327
Knoll LJ, Johnson DR, Gordon JI (1994) Biochemical
studies of three Saccharomyces cerevisiae acyl-CoA
synthetases, Faalp, FaaZp, and Faa3p. J Biol Chem
269:16348–16356
Kocharin K, Chen Y, Siewers V, Nielsen J (2012) Engineering of acetyl-CoA metabolism for the
improved production of polyhydroxybutyrate in
Saccharomyces cerevisiae. AMB Express 2:52
Kocharin K, Siewers V, Nielsen J (2013) Improved polyhydroxybutyrate production by Saccharomyces
cerevisiae through the use of the phosphoketolase
pathway. Biotechnol Bioeng 110:2216–2224
Koopman F, Beekwilder J, Crimi B, van Houwelingen A,
Hall RD, Bosch D, van Maris AJA, Pronk JT, Daran
J-M (2012) De novo production of the flavonoid
naringenin in engineered Saccharomyces cerevisiae. Microb Cell Factories 11:155
Kozak BU, van Rossum HM, Luttik MAH, Akeroyd M,
Benjamin KR, Wu L, de Vries S, Daran J-M, Pronk
JT, van Maris AJA (2014a) Engineering acetyl
coenzyme A supply: functional expression of a
bacterial pyruvate dehydrogenase complex in the
cytosol of Saccharomyces cerevisiae. MBio 5:
e01696–e01614
Kozak BU, van Rossum HM, Benjamin KR, Wu L,
Daran J-MG, Pronk JT, van Maris AJA (2014b)
Replacement of the Saccharomyces cerevisiae
acetyl-CoA synthetases by alternative pathways
for cytosolic acetyl-CoA synthesis. Metab Eng
21:46–59
Kozak BU, van Rossum HM, Niemeijer MS, van Dijk M,
Benjamin K, Wu L, Daran J-MG, Pronk JT, van
Maris AJA (2016) Replacement of the initial steps
14 Engineering Saccharomyces cerevisiae for Production of Fatty Acids and Their Derivatives
363