oxidation reversal and o-oxidation pathways for
the synthesis of medium chain o-functionalized
carboxylic acids. Metab Eng 28:202–212
Cottrell M, Viljoen BC, Kock JLF, Lategan PM (1986)
The long-chain fatty acid compositions of species
representing the genera Saccharomyces, Schwanniomyces and Lipomyces. Microbiology 132:2401–
2403
Curran KA, Karim AS, Gupta A, Alper HS (2013) Use of
expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and
improve gene expression control for metabolic
engineering applications. Metab Eng 19:88–97
d’Espaux L, Mendez-Perez D, Li R, Keasling JD (2015)
Synthetic biology for microbial production of
lipid-based biofuels. Curr Opin Chem Biol
29:58–65
d’Espaux L, Ghosh A, Runguphan W, Wehrs M, Xu F,
Konzock O, Dev I, Nhan M, Gin J, Reider Apel A,
Petzold CJ, Singh S, Simmons BA, Mukhopadhyay
A, Garcı ´a Martı ´n H, Keasling JD (2017) Engineering high-level production of fatty alcohols by Saccharomyces cerevisiae from lignocellulosic
feedstocks. Metab Eng 42:115–125
Da Silva NA, Srikrishnan S (2012) Introduction and
expression of genes for metabolic engineering
applications in Saccharomyces cerevisiae. FEMS
Yeast Res 12:197–214
David F, Nielsen J, Siewers V (2016) Flux control at the
malonyl-CoA node through hierarchical dynamic
pathway regulation in Saccharomyces cerevisiae.
ACS Synth Biol 5:224–233
Davis Lo ´pez SA, Griffith DA, Choi B, Cate JHD,
Tullman-Ercek D (2018) Evolutionary engineering
improves tolerance for medium-chain alcohols
in Saccharomyces cerevisiae. Biotechnol Biofuels
11:36
de Jong BW, Shi S, Siewers V, Nielsen J (2014)
Improved production of fatty acid ethyl esters in
Saccharomyces cerevisiae through up-regulation of
the ethanol degradation pathway and expression
of the heterologous phosphoketolase pathway.
Microb Cell Factories 13:39
Dekishima Y, Lan EI, Shen CR, Cho KM, Liao JC (2011)
Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli. J Am Chem Soc
133:11399–11401
Dellomonaco C, Clomburg JM, Miller EN, Gonzalez R
(2011) Engineered reversal of the b-oxidation
cycle for the synthesis of fuels and chemicals.
Nature 476:355–359
Deparis Q, Claes A, Foulquie ´-Moreno MR, Thevelein
JM (2017) Engineering tolerance to industrially
relevant stress factors in yeast cell factories.
FEMS Yeast Res 17:861
Dietrich JA, McKee AE, Keasling JD (2010) Highthroughput metabolic engineering: advances in
small-molecule screening and selection. Annu
Rev Biochem 79:563–590
Dragosits M, Mattanovich D (2013) Adaptive laboratory evolution—principles and applications for
biotechnology. Microb Cell Factories 12:64
Eriksen DT, HamediRad M, Yuan Y, Zhao H (2015)
Orthogonal fatty acid biosynthetic pathway
improves fatty acid ethyl ester production in Saccharomyces cerevisiae. ACS Synth Biol 4:808–814
Feng X, Lian J, Zhao H (2015) Metabolic engineering of
Saccharomyces cerevisiae to improve 1hexadecanol production. Metab Eng 27:10–19
Fernandez-Moya R, Da Silva NA (2017) Engineering
Saccharomyces cerevisiae for high-level synthesis
of fatty acids and derived products. FEMS Yeast
Res 17:87
Fernandez-Moya R, Leber C, Cardenas J, Da Silva NA
(2015) Functional replacement of the Saccharomyces cerevisiae fatty acid synthase with a bacterial
type II system allows flexible product profiles.
Biotechnol Bioeng 112:2618–2623
Foo JL, Susanto AV, Keasling JD, Leong SSJ, Chang MW
(2017) Whole-cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiae. Biotechnol Bioeng 114:232–237
Gajewski J, Pavlovic R, Fischer M, Boles E, Grininger M
(2017) Engineering fungal de novo fatty acid synthesis for short chain fatty acid production. Nat
Commun 8:14650
Gibson BR, Lawrence SJ, Leclaire JPR, Powell CD,
Smart KA (2007) Yeast responses to stresses associated with industrial brewery handling. FEMS
Microbiol Rev 31:535–569
Gonza ´lez-Ramos D, van den Broek M, van Maris AJA,
Pronk JT, Daran J-MG (2013) Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein
degradation. Biotechnol Biofuels 6:48
Gonza ´lez-Ramos D, Gorter de Vries AR, Grijseels SS,
van Berkum MC, Swinnen S, van den Broek M,
Nevoigt E, Daran J-MG, Pronk JT, van Maris AJA
(2016) A new laboratory evolution approach to
select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal
mutations. Biotechnol Biofuels 9:19
Gossing M, Smialowska A, Nielsen J (2018) Impact of
forced fatty acid synthesis on metabolism and
physiology of Saccharomyces cerevisiae. FEMS
Yeast Res 18:153
Han L, Peng Y, Zhang Y, Chen W, Lin Y, Wang Q (2017)
Designing and creating a synthetic omega oxidation pathway in Saccharomyces cerevisiae enables
production of medium-chain a, o-dicarboxylic
acids. Front Microbiol 8:1123
Hanscho M, Ruckerbauer DE, Chauhan N, Hofbauer
HF, Krahulec S, Nidetzky B, Kohlwein SD, Zanghellini J, Natter K (2012) Nutritional requirements of the BY series of Saccharomyces
362
L. Baumann et al.
the synthesis of medium chain o-functionalized
carboxylic acids. Metab Eng 28:202–212
Cottrell M, Viljoen BC, Kock JLF, Lategan PM (1986)
The long-chain fatty acid compositions of species
representing the genera Saccharomyces, Schwanniomyces and Lipomyces. Microbiology 132:2401–
2403
Curran KA, Karim AS, Gupta A, Alper HS (2013) Use of
expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and
improve gene expression control for metabolic
engineering applications. Metab Eng 19:88–97
d’Espaux L, Mendez-Perez D, Li R, Keasling JD (2015)
Synthetic biology for microbial production of
lipid-based biofuels. Curr Opin Chem Biol
29:58–65
d’Espaux L, Ghosh A, Runguphan W, Wehrs M, Xu F,
Konzock O, Dev I, Nhan M, Gin J, Reider Apel A,
Petzold CJ, Singh S, Simmons BA, Mukhopadhyay
A, Garcı ´a Martı ´n H, Keasling JD (2017) Engineering high-level production of fatty alcohols by Saccharomyces cerevisiae from lignocellulosic
feedstocks. Metab Eng 42:115–125
Da Silva NA, Srikrishnan S (2012) Introduction and
expression of genes for metabolic engineering
applications in Saccharomyces cerevisiae. FEMS
Yeast Res 12:197–214
David F, Nielsen J, Siewers V (2016) Flux control at the
malonyl-CoA node through hierarchical dynamic
pathway regulation in Saccharomyces cerevisiae.
ACS Synth Biol 5:224–233
Davis Lo ´pez SA, Griffith DA, Choi B, Cate JHD,
Tullman-Ercek D (2018) Evolutionary engineering
improves tolerance for medium-chain alcohols
in Saccharomyces cerevisiae. Biotechnol Biofuels
11:36
de Jong BW, Shi S, Siewers V, Nielsen J (2014)
Improved production of fatty acid ethyl esters in
Saccharomyces cerevisiae through up-regulation of
the ethanol degradation pathway and expression
of the heterologous phosphoketolase pathway.
Microb Cell Factories 13:39
Dekishima Y, Lan EI, Shen CR, Cho KM, Liao JC (2011)
Extending carbon chain length of 1-butanol pathway for 1-hexanol synthesis from glucose by engineered Escherichia coli. J Am Chem Soc
133:11399–11401
Dellomonaco C, Clomburg JM, Miller EN, Gonzalez R
(2011) Engineered reversal of the b-oxidation
cycle for the synthesis of fuels and chemicals.
Nature 476:355–359
Deparis Q, Claes A, Foulquie ´-Moreno MR, Thevelein
JM (2017) Engineering tolerance to industrially
relevant stress factors in yeast cell factories.
FEMS Yeast Res 17:861
Dietrich JA, McKee AE, Keasling JD (2010) Highthroughput metabolic engineering: advances in
small-molecule screening and selection. Annu
Rev Biochem 79:563–590
Dragosits M, Mattanovich D (2013) Adaptive laboratory evolution—principles and applications for
biotechnology. Microb Cell Factories 12:64
Eriksen DT, HamediRad M, Yuan Y, Zhao H (2015)
Orthogonal fatty acid biosynthetic pathway
improves fatty acid ethyl ester production in Saccharomyces cerevisiae. ACS Synth Biol 4:808–814
Feng X, Lian J, Zhao H (2015) Metabolic engineering of
Saccharomyces cerevisiae to improve 1hexadecanol production. Metab Eng 27:10–19
Fernandez-Moya R, Da Silva NA (2017) Engineering
Saccharomyces cerevisiae for high-level synthesis
of fatty acids and derived products. FEMS Yeast
Res 17:87
Fernandez-Moya R, Leber C, Cardenas J, Da Silva NA
(2015) Functional replacement of the Saccharomyces cerevisiae fatty acid synthase with a bacterial
type II system allows flexible product profiles.
Biotechnol Bioeng 112:2618–2623
Foo JL, Susanto AV, Keasling JD, Leong SSJ, Chang MW
(2017) Whole-cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiae. Biotechnol Bioeng 114:232–237
Gajewski J, Pavlovic R, Fischer M, Boles E, Grininger M
(2017) Engineering fungal de novo fatty acid synthesis for short chain fatty acid production. Nat
Commun 8:14650
Gibson BR, Lawrence SJ, Leclaire JPR, Powell CD,
Smart KA (2007) Yeast responses to stresses associated with industrial brewery handling. FEMS
Microbiol Rev 31:535–569
Gonza ´lez-Ramos D, van den Broek M, van Maris AJA,
Pronk JT, Daran J-MG (2013) Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein
degradation. Biotechnol Biofuels 6:48
Gonza ´lez-Ramos D, Gorter de Vries AR, Grijseels SS,
van Berkum MC, Swinnen S, van den Broek M,
Nevoigt E, Daran J-MG, Pronk JT, van Maris AJA
(2016) A new laboratory evolution approach to
select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal
mutations. Biotechnol Biofuels 9:19
Gossing M, Smialowska A, Nielsen J (2018) Impact of
forced fatty acid synthesis on metabolism and
physiology of Saccharomyces cerevisiae. FEMS
Yeast Res 18:153
Han L, Peng Y, Zhang Y, Chen W, Lin Y, Wang Q (2017)
Designing and creating a synthetic omega oxidation pathway in Saccharomyces cerevisiae enables
production of medium-chain a, o-dicarboxylic
acids. Front Microbiol 8:1123
Hanscho M, Ruckerbauer DE, Chauhan N, Hofbauer
HF, Krahulec S, Nidetzky B, Kohlwein SD, Zanghellini J, Natter K (2012) Nutritional requirements of the BY series of Saccharomyces
362
L. Baumann et al.