Nielsen J, Larsson C, van Maris A, Pronk J (2013)
Metabolic engineering of yeast for production of
fuels and chemicals. Curr Opin Biotechnol 24:398–
404
Nyga ˚rd Y, Mojzita D, Toivari M, Penttila ¨ M, Wiebe MG,
Ruohonen L (2014) The diverse role of Pdr12 in
resistance to weak organic acids. Yeast 31:219–232
Oelkers P, Tinkelenberg A, Erdeniz N, Cromley D, Billheimer JT, Sturley SL (2000) A lecithin cholesterol
acyltransferase-like gene mediates diacylglycerol
esterification in yeast. J Biol Chem 275:15609–
15612
Oelkers P, Cromley D, Padamsee M, Billheimer JT,
Sturley SL (2002) The DGA1 gene determines a
second triglyceride synthetic pathway in yeast. J
Biol Chem 277:8877–8881
Oh CS, Toke DA, Mandala S, Martin CE (1997) ELO2
and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J
Biol Chem 272:17376–17384
Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K,
McPhee D, Leavell MD, Tai A, Main A, Eng D,
Polichuk DR, Teoh KH, Reed DW, Treynor T,
Lenihan J, Jiang H, Fleck M, Bajad S, Dang G,
Dengrove D, Diola D, Dorin G, Ellens KW, Fickes
S, Galazzo J, Gaucher SP, Geistlinger T, Henry R,
Hepp M, Horning T, Iqbal T, Kizer L, Lieu B, Melis
D, Moss N, Regentin R, Secrest S, Tsuruta H,
Vazquez R, Westblade LF, Xu L, Yu M, Zhang Y,
Zhao L, Lievense J, Covello PS, Keasling JD, Reiling KK, Renninger NS, Newman JD (2013) Highlevel semi-synthetic production of the potent antimalarial artemisinin. Nature 496:528–532
Peralta-Yahya PP, Zhang F, del Cardayre SB, Keasling
JD (2012) Microbial engineering for the production of advanced biofuels. Nature 488:320–328
Pfleger BF, Gossing M, Nielsen J (2015) Metabolic engineering strategies for microbial synthesis of oleochemicals. Metab Eng 29:1–11
Pham TK, Wright PC (2008) The proteomic response of
Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation. J Proteome Res 7:4766–4774
Piper PW (1995) The heat shock and ethanol stress
responses of yeast exhibit extensive similarity
and functional overlap. FEMS Microbiol Lett
134:121–127
Pronk JT, Yde Steensma H, van Dijken JP (1996) Pyruvate metabolism in Saccharomyces cerevisiae.
Yeast 12:1607–1633
Rigouin C, Gueroult M, Croux C, Dubois G, Borsenberger V, Barbe S, Marty A, Daboussi F, Andre ´ I,
Bordes F (2017) Production of medium chain
Fatty acids by Yarrowia lipolytica: combining
molecular design and TALEN to engineer the
fatty acid synthase. ACS Synth Biol 6:1870–1879
Rodriguez S, Denby CM, van Vu T, Baidoo EEK, Wang
G, Keasling JD (2016) ATP citrate lyase mediated
cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae.
Microb Cell Factories 15:169
Royce LA, Yoon JM, Chen Y, Rickenbach E, Shanks JV,
Jarboe LR (2015) Evolution for exogenous octanoic acid tolerance improves carboxylic acid production and membrane integrity. Metab Eng
29:180–188
Runguphan W, Keasling JD (2014) Metabolic engineering of Saccharomyces cerevisiae for production of
fatty acid-derived biofuels and chemicals. Metab
Eng 21:103–113
Rupilius W, Ahma S (2006) The changing world of
oleochemicals. Palm Oil Dev 44:21–28
Sandoval CM, Ayson M, Moss N, Lieu B, Jackson P,
Gaucher SP, Horning T, Dahl RH, Denery JR,
Abbott DA, Meadows AL (2014) Use of pantothenate as a metabolic switch increases the genetic
stability of farnesene producing Saccharomyces
cerevisiae. Metab Eng 25:215–226
Santamauro F, Whiffin FM, Scott RJ, Chuck CJ (2014)
Low-cost lipid production by an oleaginous yeast
cultured in non-sterile conditions using model
waste resources. Biotechnol Biofuels 7:34
Sanz P (2003) Snf1 protein kinase: a key player in the
response to cellular stress in yeast. Biochem Soc
Trans 31:178–181
Sarria S, Kruyer NS, Peralta-Yahya P (2017) Microbial
synthesis of medium-chain chemicals from renewables. Nat Biotechnol 35:1158–1166
Schadeweg V, Boles E (2016a) Increasing n-butanol
production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and
trans-2-enoyl-CoA reductase expression. Biotechnol Biofuels 9:1
Schadeweg V, Boles E (2016b) n-Butanol production in
Saccharomyces cerevisiae is limited by the availability of coenzyme A and cytosolic acetyl-CoA.
Biotechnol Biofuels 9:1
Schallmey M, Frunzke J, Eggeling L (2014) Looking for
the pick of the bunch: high-throughput screening
of producing microorganisms with biosensors.
Curr Opin Biotechnol 26:148–154
Scharnewski M, Pongdontri P, Mora G, Hoppert M,
Fulda M (2008) Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty
acids due to interrupted fatty acid recycling. FEBS
J 275:2765–2778
Schmidt JH (2015) Life cycle assessment of five vegetable oils. J Clean Prod 87:130–138
Schweizer E, Hofmann J (2004) Microbial type I fatty
acid synthases (FAS): major players in a network
of cellular FAS systems. Microbiol Mol Biol Rev
68:501–517. Table of contents
Sekine T, Kawaguchi A, Hamano Y, Takagi H (2007)
Desensitization of feedback inhibition of the Saccharomyces cerevisiae g-glutamyl kinase enhances
proline accumulation and freezing tolerance. Appl
Environ Microbiol 73:4011–4019
14 Engineering Saccharomyces cerevisiae for Production of Fatty Acids and Their Derivatives
365
Metabolic engineering of yeast for production of
fuels and chemicals. Curr Opin Biotechnol 24:398–
404
Nyga ˚rd Y, Mojzita D, Toivari M, Penttila ¨ M, Wiebe MG,
Ruohonen L (2014) The diverse role of Pdr12 in
resistance to weak organic acids. Yeast 31:219–232
Oelkers P, Tinkelenberg A, Erdeniz N, Cromley D, Billheimer JT, Sturley SL (2000) A lecithin cholesterol
acyltransferase-like gene mediates diacylglycerol
esterification in yeast. J Biol Chem 275:15609–
15612
Oelkers P, Cromley D, Padamsee M, Billheimer JT,
Sturley SL (2002) The DGA1 gene determines a
second triglyceride synthetic pathway in yeast. J
Biol Chem 277:8877–8881
Oh CS, Toke DA, Mandala S, Martin CE (1997) ELO2
and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J
Biol Chem 272:17376–17384
Paddon CJ, Westfall PJ, Pitera DJ, Benjamin K, Fisher K,
McPhee D, Leavell MD, Tai A, Main A, Eng D,
Polichuk DR, Teoh KH, Reed DW, Treynor T,
Lenihan J, Jiang H, Fleck M, Bajad S, Dang G,
Dengrove D, Diola D, Dorin G, Ellens KW, Fickes
S, Galazzo J, Gaucher SP, Geistlinger T, Henry R,
Hepp M, Horning T, Iqbal T, Kizer L, Lieu B, Melis
D, Moss N, Regentin R, Secrest S, Tsuruta H,
Vazquez R, Westblade LF, Xu L, Yu M, Zhang Y,
Zhao L, Lievense J, Covello PS, Keasling JD, Reiling KK, Renninger NS, Newman JD (2013) Highlevel semi-synthetic production of the potent antimalarial artemisinin. Nature 496:528–532
Peralta-Yahya PP, Zhang F, del Cardayre SB, Keasling
JD (2012) Microbial engineering for the production of advanced biofuels. Nature 488:320–328
Pfleger BF, Gossing M, Nielsen J (2015) Metabolic engineering strategies for microbial synthesis of oleochemicals. Metab Eng 29:1–11
Pham TK, Wright PC (2008) The proteomic response of
Saccharomyces cerevisiae in very high glucose conditions with amino acid supplementation. J Proteome Res 7:4766–4774
Piper PW (1995) The heat shock and ethanol stress
responses of yeast exhibit extensive similarity
and functional overlap. FEMS Microbiol Lett
134:121–127
Pronk JT, Yde Steensma H, van Dijken JP (1996) Pyruvate metabolism in Saccharomyces cerevisiae.
Yeast 12:1607–1633
Rigouin C, Gueroult M, Croux C, Dubois G, Borsenberger V, Barbe S, Marty A, Daboussi F, Andre ´ I,
Bordes F (2017) Production of medium chain
Fatty acids by Yarrowia lipolytica: combining
molecular design and TALEN to engineer the
fatty acid synthase. ACS Synth Biol 6:1870–1879
Rodriguez S, Denby CM, van Vu T, Baidoo EEK, Wang
G, Keasling JD (2016) ATP citrate lyase mediated
cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae.
Microb Cell Factories 15:169
Royce LA, Yoon JM, Chen Y, Rickenbach E, Shanks JV,
Jarboe LR (2015) Evolution for exogenous octanoic acid tolerance improves carboxylic acid production and membrane integrity. Metab Eng
29:180–188
Runguphan W, Keasling JD (2014) Metabolic engineering of Saccharomyces cerevisiae for production of
fatty acid-derived biofuels and chemicals. Metab
Eng 21:103–113
Rupilius W, Ahma S (2006) The changing world of
oleochemicals. Palm Oil Dev 44:21–28
Sandoval CM, Ayson M, Moss N, Lieu B, Jackson P,
Gaucher SP, Horning T, Dahl RH, Denery JR,
Abbott DA, Meadows AL (2014) Use of pantothenate as a metabolic switch increases the genetic
stability of farnesene producing Saccharomyces
cerevisiae. Metab Eng 25:215–226
Santamauro F, Whiffin FM, Scott RJ, Chuck CJ (2014)
Low-cost lipid production by an oleaginous yeast
cultured in non-sterile conditions using model
waste resources. Biotechnol Biofuels 7:34
Sanz P (2003) Snf1 protein kinase: a key player in the
response to cellular stress in yeast. Biochem Soc
Trans 31:178–181
Sarria S, Kruyer NS, Peralta-Yahya P (2017) Microbial
synthesis of medium-chain chemicals from renewables. Nat Biotechnol 35:1158–1166
Schadeweg V, Boles E (2016a) Increasing n-butanol
production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and
trans-2-enoyl-CoA reductase expression. Biotechnol Biofuels 9:1
Schadeweg V, Boles E (2016b) n-Butanol production in
Saccharomyces cerevisiae is limited by the availability of coenzyme A and cytosolic acetyl-CoA.
Biotechnol Biofuels 9:1
Schallmey M, Frunzke J, Eggeling L (2014) Looking for
the pick of the bunch: high-throughput screening
of producing microorganisms with biosensors.
Curr Opin Biotechnol 26:148–154
Scharnewski M, Pongdontri P, Mora G, Hoppert M,
Fulda M (2008) Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty
acids due to interrupted fatty acid recycling. FEBS
J 275:2765–2778
Schmidt JH (2015) Life cycle assessment of five vegetable oils. J Clean Prod 87:130–138
Schweizer E, Hofmann J (2004) Microbial type I fatty
acid synthases (FAS): major players in a network
of cellular FAS systems. Microbiol Mol Biol Rev
68:501–517. Table of contents
Sekine T, Kawaguchi A, Hamano Y, Takagi H (2007)
Desensitization of feedback inhibition of the Saccharomyces cerevisiae g-glutamyl kinase enhances
proline accumulation and freezing tolerance. Appl
Environ Microbiol 73:4011–4019
14 Engineering Saccharomyces cerevisiae for Production of Fatty Acids and Their Derivatives
365