186
the Fermentation Factory Group of Chinoin including Biotechnology Development
Department was signed in 1976. Further, both cyclodextrin glucosyltransferase
preparation and cyclodextrin production proceeded in close cooperation of research
and development members. Milestones of the technology development are summarized in chronological order in Fig. 4.3.
4.2.1 Development of Enzyme Production
Selection of a Cyclodextrin Glucosyltransferase Enzyme-Producing
Microorganism and Optimizing the Cultivation Conditions
Cyclodextrin glucosyltransferase is a unique enzyme, which reacts with starchy
substrates with three action mechanisms: cyclizing, transglucosylation (coupling
and disproportionation), and hydrolyzing. It should be noted that cyclodextrin glucosyltransferases always produce alpha-cyclodextrin as primary cyclization product, which is transformed into beta-cyclodextrin and gamma-cyclodextrin in parallel
due to coupling and cyclization reactions proceeding mainly in late stage of starch
conversion.
1976
1977
1978
1979
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
Lab-scale development of the conversion for BCD
using Bacillus macerans, Patent applicaƟon
Scaling up to 3 m
3 using B. macerans
Patent applicaƟon for enzyme immobilizaƟon
Lab-scale development of the conversion for GCD
Lyophilized CGTase enzyme for sale
Development of toluene-free technology
Scaling up of BCD conversion to 8 m
3
DocumentaƟon (SOP) of the technology
Scaling up of enzyme producƟon (3 m
3
)
Pilot plant producƟon of ACD
Industrial producƟon of BCD
Patent applicaƟons for CGTase producƟon
DocumentaƟon of technology for
concentrate
Patent applicaƟon for ACD and GCD producƟon
Pilot-plant scale producƟon of GCD
110 kg BCD
400 kg BCD
360 kg ACD
20 tons BCD
11 kg GCD
Lab-scale development of enzyme producƟon
DocumentaƟon (SOP) of technology for purified
enzyme
Technology transfer to Győr DisƟllery Co Ltd.
Fig. 4.3 Chronology of technology development for production of alpha-cyclodextrin (ACD),
beta-cyclodextrin (BCD), and gamma-cyclodextrin (GCD) and cyclodextrin glucosyltransferase
(CGTase)
É. Fenyvesi et al.
the Fermentation Factory Group of Chinoin including Biotechnology Development
Department was signed in 1976. Further, both cyclodextrin glucosyltransferase
preparation and cyclodextrin production proceeded in close cooperation of research
and development members. Milestones of the technology development are summarized in chronological order in Fig. 4.3.
4.2.1 Development of Enzyme Production
Selection of a Cyclodextrin Glucosyltransferase Enzyme-Producing
Microorganism and Optimizing the Cultivation Conditions
Cyclodextrin glucosyltransferase is a unique enzyme, which reacts with starchy
substrates with three action mechanisms: cyclizing, transglucosylation (coupling
and disproportionation), and hydrolyzing. It should be noted that cyclodextrin glucosyltransferases always produce alpha-cyclodextrin as primary cyclization product, which is transformed into beta-cyclodextrin and gamma-cyclodextrin in parallel
due to coupling and cyclization reactions proceeding mainly in late stage of starch
conversion.
1976
1977
1978
1979
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
Lab-scale development of the conversion for BCD
using Bacillus macerans, Patent applicaƟon
Scaling up to 3 m
3 using B. macerans
Patent applicaƟon for enzyme immobilizaƟon
Lab-scale development of the conversion for GCD
Lyophilized CGTase enzyme for sale
Development of toluene-free technology
Scaling up of BCD conversion to 8 m
3
DocumentaƟon (SOP) of the technology
Scaling up of enzyme producƟon (3 m
3
)
Pilot plant producƟon of ACD
Industrial producƟon of BCD
Patent applicaƟons for CGTase producƟon
DocumentaƟon of technology for
concentrate
Patent applicaƟon for ACD and GCD producƟon
Pilot-plant scale producƟon of GCD
110 kg BCD
400 kg BCD
360 kg ACD
20 tons BCD
11 kg GCD
Lab-scale development of enzyme producƟon
DocumentaƟon (SOP) of technology for purified
enzyme
Technology transfer to Győr DisƟllery Co Ltd.
Fig. 4.3 Chronology of technology development for production of alpha-cyclodextrin (ACD),
beta-cyclodextrin (BCD), and gamma-cyclodextrin (GCD) and cyclodextrin glucosyltransferase
(CGTase)
É. Fenyvesi et al.