131. Bai W, Xue Y, Zhou C, Ma Y (2015) Cloning, expression, and characterization of a novel
alkali-tolerant xylanase from alkaliphilic Bacillus sp. SN5. Biotechnol Appl Biochem
62:208–217
132. Henrissat B, Bairoch A (1996) Updating the sequence-based classification of glycosyl
hydrolases. Biochem J 316:695–696
133. Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A (2003) First crystallographic
structure of a xylanase from glycoside hydrolase family 5: implications for catalysis.
Biochemistry 42:8411–8422
134. Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, Van Beeumen J (2003) The
structure of a cold-adapted family 8 xylanase at 1.3 A resolution. Structural adaptations to cold
and investigation of the active site. J Biol Chem 278:7531–7539
135. Mamo G, Thunnissen M, Hatti-Kaul R, Mattiasson B (2009) An alkaline active xylanase:
insights into mechanisms of high pH catalytic adaptation. Biochimie 91:1187–1196
136. St John FJ, Hurlbert JC, Rice JD, Preston JF, Pozharski E (2011) Ligand bound structures of a
glycosyl hydrolase family 30 glucuronoxylan xylanohydrolase. J Mol Biol 407:92–109
137. Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G et al (2009)
Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus
stearothermophilus complexed with its substrate. Biochem J 422:73–82
138. Im DH, Kimura KI, Hayasaka F, Tanaka T, Noguchi M, Kobayashi A et al (2012) Crystal
structures of glycoside hydrolase family 51 alpha-L-arabinofuranosidase from Thermotoga
maritima. Biosci Biotechnol Biochem 76:423–428
139. Higgins MA, Whitworth GE, El Warry N, Randriantsoa M, Samain E, Burke RD et al (2009)
Differential recognition and hydrolysis of host carbohydrate-antigens by Streptococcus
Pneumoniae family 98 glycoside hydrolases. J Biol Chem 284:26161–26171
140. Zhao Y, Zhang Y, Cao Y, Qi J, Mao L, Xue Y et al (2011) Structural analysis of alkaline
β-mannanase from alkaliphilic Bacillus sp. N16-5: implications for adaptation to alkaline
conditions. PLoS One 6(1):e14608
141. You X, Qin Z, Yan Q, Yang SQ, Li Y, Jiang ZQ (2018) Complex structure of GH113 beta-1,4mannanase. J Biol Chem 293:11746–11757
142. Jin Y, Petricevic M, John A, Raich L, Jenkins H, Portela De Souza L et al (2016) A betamannanase with a lysozyme-like fold and a novel molecular catalytic mechanism. ACS Cent
Sci 2:896–903
143. Farber GK, Petsko GA (1990) The evolution of α/β barrel enzymes. Trends Biochem Sci
15:228–234
144. Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS et al (2006) Crystal
structures of native and xylosaccharide-bound alkali thermostable xylanase from an
alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for
adaptation to polyextreme conditions. Protein Sci 15:1951–1960
145. Bai W, Zhou C, Xue Y, Huang CH, Guo RT, Ma Y (2014) Three-dimensional structure of an
alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its
thermal performance by introducing arginines substitutions. Biotechnol Lett 36:1495–1501
146. Davies G, Henrissat B (1995) Structures and mechanisms of glycosyl hydrolases. Structure
3:853–859
147. Biely P, Vrsanska M, Tenkanen M, Kluepfel D (1999) Endo-β-1,4-xylanase families: differences in catalytic properties. J Biotechnol 57:151–166
148. Leggio LL, Jenkins J, Harris GW, Pickersgill RW (2000) X-ray crystallographic study of
xylopentaose binding to Pseudomonas fluorescens xylanase A. Proteins Struct Funct Genet
41:362–373
149. Shimizu M, Kaneko Y, Ishihara S, Mochizuki M, Sakai K, Yamada M et al (2015) Novel
β-1,4-mannanase belonging to a new glycoside hydrolase family in Aspergillus nidulans.
J Biol Chem 290:27914–27927
150. Li S, Yang X, Yang S, Zhu M, Wang X (2012) Technology prospecting on enzymes:
application, marketing and engineering. Comput Struct Biotechnol J 2:e201209017
Alkaline Active Hemicellulases
287
alkali-tolerant xylanase from alkaliphilic Bacillus sp. SN5. Biotechnol Appl Biochem
62:208–217
132. Henrissat B, Bairoch A (1996) Updating the sequence-based classification of glycosyl
hydrolases. Biochem J 316:695–696
133. Larson SB, Day J, Barba de la Rosa AP, Keen NT, McPherson A (2003) First crystallographic
structure of a xylanase from glycoside hydrolase family 5: implications for catalysis.
Biochemistry 42:8411–8422
134. Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, Van Beeumen J (2003) The
structure of a cold-adapted family 8 xylanase at 1.3 A resolution. Structural adaptations to cold
and investigation of the active site. J Biol Chem 278:7531–7539
135. Mamo G, Thunnissen M, Hatti-Kaul R, Mattiasson B (2009) An alkaline active xylanase:
insights into mechanisms of high pH catalytic adaptation. Biochimie 91:1187–1196
136. St John FJ, Hurlbert JC, Rice JD, Preston JF, Pozharski E (2011) Ligand bound structures of a
glycosyl hydrolase family 30 glucuronoxylan xylanohydrolase. J Mol Biol 407:92–109
137. Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G et al (2009)
Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus
stearothermophilus complexed with its substrate. Biochem J 422:73–82
138. Im DH, Kimura KI, Hayasaka F, Tanaka T, Noguchi M, Kobayashi A et al (2012) Crystal
structures of glycoside hydrolase family 51 alpha-L-arabinofuranosidase from Thermotoga
maritima. Biosci Biotechnol Biochem 76:423–428
139. Higgins MA, Whitworth GE, El Warry N, Randriantsoa M, Samain E, Burke RD et al (2009)
Differential recognition and hydrolysis of host carbohydrate-antigens by Streptococcus
Pneumoniae family 98 glycoside hydrolases. J Biol Chem 284:26161–26171
140. Zhao Y, Zhang Y, Cao Y, Qi J, Mao L, Xue Y et al (2011) Structural analysis of alkaline
β-mannanase from alkaliphilic Bacillus sp. N16-5: implications for adaptation to alkaline
conditions. PLoS One 6(1):e14608
141. You X, Qin Z, Yan Q, Yang SQ, Li Y, Jiang ZQ (2018) Complex structure of GH113 beta-1,4mannanase. J Biol Chem 293:11746–11757
142. Jin Y, Petricevic M, John A, Raich L, Jenkins H, Portela De Souza L et al (2016) A betamannanase with a lysozyme-like fold and a novel molecular catalytic mechanism. ACS Cent
Sci 2:896–903
143. Farber GK, Petsko GA (1990) The evolution of α/β barrel enzymes. Trends Biochem Sci
15:228–234
144. Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS et al (2006) Crystal
structures of native and xylosaccharide-bound alkali thermostable xylanase from an
alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for
adaptation to polyextreme conditions. Protein Sci 15:1951–1960
145. Bai W, Zhou C, Xue Y, Huang CH, Guo RT, Ma Y (2014) Three-dimensional structure of an
alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its
thermal performance by introducing arginines substitutions. Biotechnol Lett 36:1495–1501
146. Davies G, Henrissat B (1995) Structures and mechanisms of glycosyl hydrolases. Structure
3:853–859
147. Biely P, Vrsanska M, Tenkanen M, Kluepfel D (1999) Endo-β-1,4-xylanase families: differences in catalytic properties. J Biotechnol 57:151–166
148. Leggio LL, Jenkins J, Harris GW, Pickersgill RW (2000) X-ray crystallographic study of
xylopentaose binding to Pseudomonas fluorescens xylanase A. Proteins Struct Funct Genet
41:362–373
149. Shimizu M, Kaneko Y, Ishihara S, Mochizuki M, Sakai K, Yamada M et al (2015) Novel
β-1,4-mannanase belonging to a new glycoside hydrolase family in Aspergillus nidulans.
J Biol Chem 290:27914–27927
150. Li S, Yang X, Yang S, Zhu M, Wang X (2012) Technology prospecting on enzymes:
application, marketing and engineering. Comput Struct Biotechnol J 2:e201209017
Alkaline Active Hemicellulases
287