Hydrophobic effect, 2
Hydrophobic groups, 2
Hydrophobic sink, 290, 296
Hydrophobized poly-γ-glutamic acid, 119
I
IBs, see Inclusion bodies (IBs)
IFU, see Inclusion forming units (C. muridarum)
IM, see Ion mobility (IM) cell
IMAC, see Immobilized-metal affinity chromatography
(IMAC)
Immobilized-metal affinity chromatography (IMAC), 257,
310, 317, 321
Inclusion bodies (IBs), 334
Infra-red absorption vs. Raman scattering
spectroscopies, 395
Infra-red spectroscopies, 395
Insulin receptor, 261, 262
Insulin-responsive facilitative glucose transporter
(GLUT4), 292, 293
APol-assisted folding, 340
improved stability in A8-35 vs. LMNG, 475
Integrins
ND-trapped, 116
Interactome
APol-assisted recovery, 647
Inverted micelles, 5
Ion mobility (IM) cell
principle, 627
J
Jonas, A., 105
Julius, D., 564, 579
K
KcsA potassium channel
engineering a water-soluble version, 88
folding in NDs, 118
specific binding of PG, 27, 29
3D structure, 20
TM structure, 16
X-ray structure, 20
Khorana, H.G., 334, 335
Klebsiella pneumoniae, 276
Kleinschmidt, J.H., 294, 308, 349
Kp-tOmpA, see OmpA (K. pneumoniae) transmembrane
domain
Kühlbrandt, W., 562
L
Lactose permease
crystallization, 76
Ladokhin, A.S., 308
Landau, E.M., 506
Lauryl-, see DodecylLauryldimethylamine oxide (LDAO), 64
chemical structure, 61
CMC, 61
Lauryl maltose neopentyl glycol (LMNG), 83, 282, 292
chemical structure, 83
LC, see Liquid chromatography
LDAO, see Lauryldimethylamine oxide (LDAO)
Le Bon, C., 291, 446
Lecithin cholesteryl acetyltransferase, 107
Leucine transporter LeuT
intrusion of DDM into the TM region, 79
Leukotriene B 4
chemical structure, 486
NMR structure when bound to A8-35-trapped BLT2,
486, 487
Leukotriene BLT1 receptor, 287
APol-assisted folding, 340, 345
stabilization by APols, 292, 293
Leukotriene BLT2 receptor, 287
APol-assisted folding, 340, 345
ND-trapped, modulation by cholesterol, 116
15
N1
H CRINEPT spectra in the presence or absence
of LTB 4 , 487
NMR study of the conformation of LTB 4 bound to
A8-35-trapped BLT2, 483–487
transfer from APols to nanodiscs, 322
LHC, see Light-harvesting complex
LHCII/A8-35 complexes
SGSV analysis, 414, 416
LHCII/APol complexes
comparison with LHCII/DDM ones by SGSV
analysis, 416
LHCII/NAPol complexes
SGSV analysis, 416
Light-harvesting complex 2, bacterial
TM structure, 16
Light-harvesting complex II, eukaryotic
TM structure, 16
Lipid A palmitoyl transferase (PagP), 260
activity in SMALPs, 289
activity in various APols, 289
APol-assisted folding, 340, 348, 349
comparative ESI-MS analyses of various PagP/APol
complexes, 639
ESI-MS analyses of PagP/A8-35 complexes, 637
SEC analysis in SMALPs, 260
stability in SMALPs, 294
trapping by lipopeptide detergents, 121
Lipid/APol mixed micelles, 264
Lipid bilayers, 4–8
accessibility to water, 7
balance of forces, 7
internal lateral pressure, 8
MD model, 7
modulation of thickness and fluidity, 6
physical-chemical properties of the core region, 8, 9
Lipid black films, 308
Lipidic mesophases, 134, 310, 506
cubic phases, 506–508, 518
formation in the presence of APols, 520
sponge phases, 506, 507
Index
697
Hydrophobic groups, 2
Hydrophobic sink, 290, 296
Hydrophobized poly-γ-glutamic acid, 119
I
IBs, see Inclusion bodies (IBs)
IFU, see Inclusion forming units (C. muridarum)
IM, see Ion mobility (IM) cell
IMAC, see Immobilized-metal affinity chromatography
(IMAC)
Immobilized-metal affinity chromatography (IMAC), 257,
310, 317, 321
Inclusion bodies (IBs), 334
Infra-red absorption vs. Raman scattering
spectroscopies, 395
Infra-red spectroscopies, 395
Insulin receptor, 261, 262
Insulin-responsive facilitative glucose transporter
(GLUT4), 292, 293
APol-assisted folding, 340
improved stability in A8-35 vs. LMNG, 475
Integrins
ND-trapped, 116
Interactome
APol-assisted recovery, 647
Inverted micelles, 5
Ion mobility (IM) cell
principle, 627
J
Jonas, A., 105
Julius, D., 564, 579
K
KcsA potassium channel
engineering a water-soluble version, 88
folding in NDs, 118
specific binding of PG, 27, 29
3D structure, 20
TM structure, 16
X-ray structure, 20
Khorana, H.G., 334, 335
Klebsiella pneumoniae, 276
Kleinschmidt, J.H., 294, 308, 349
Kp-tOmpA, see OmpA (K. pneumoniae) transmembrane
domain
Kühlbrandt, W., 562
L
Lactose permease
crystallization, 76
Ladokhin, A.S., 308
Landau, E.M., 506
Lauryl-, see DodecylLauryldimethylamine oxide (LDAO), 64
chemical structure, 61
CMC, 61
Lauryl maltose neopentyl glycol (LMNG), 83, 282, 292
chemical structure, 83
LC, see Liquid chromatography
LDAO, see Lauryldimethylamine oxide (LDAO)
Le Bon, C., 291, 446
Lecithin cholesteryl acetyltransferase, 107
Leucine transporter LeuT
intrusion of DDM into the TM region, 79
Leukotriene B 4
chemical structure, 486
NMR structure when bound to A8-35-trapped BLT2,
486, 487
Leukotriene BLT1 receptor, 287
APol-assisted folding, 340, 345
stabilization by APols, 292, 293
Leukotriene BLT2 receptor, 287
APol-assisted folding, 340, 345
ND-trapped, modulation by cholesterol, 116
15
N1
H CRINEPT spectra in the presence or absence
of LTB 4 , 487
NMR study of the conformation of LTB 4 bound to
A8-35-trapped BLT2, 483–487
transfer from APols to nanodiscs, 322
LHC, see Light-harvesting complex
LHCII/A8-35 complexes
SGSV analysis, 414, 416
LHCII/APol complexes
comparison with LHCII/DDM ones by SGSV
analysis, 416
LHCII/NAPol complexes
SGSV analysis, 416
Light-harvesting complex 2, bacterial
TM structure, 16
Light-harvesting complex II, eukaryotic
TM structure, 16
Lipid A palmitoyl transferase (PagP), 260
activity in SMALPs, 289
activity in various APols, 289
APol-assisted folding, 340, 348, 349
comparative ESI-MS analyses of various PagP/APol
complexes, 639
ESI-MS analyses of PagP/A8-35 complexes, 637
SEC analysis in SMALPs, 260
stability in SMALPs, 294
trapping by lipopeptide detergents, 121
Lipid/APol mixed micelles, 264
Lipid bilayers, 4–8
accessibility to water, 7
balance of forces, 7
internal lateral pressure, 8
MD model, 7
modulation of thickness and fluidity, 6
physical-chemical properties of the core region, 8, 9
Lipid black films, 308
Lipidic mesophases, 134, 310, 506
cubic phases, 506–508, 518
formation in the presence of APols, 520
sponge phases, 506, 507
Index
697