Lipid rebinding upon transfer of MPs to APols, 305
Lipids, 2, 3
amphipathic character, 2
chemical structure of some polar lipids, 3
self-association in water, 4
Lipodisqs
®
, 164
See also styrenealeic acid co-polymers
Lipopeptide detergents, 118, 119, 134
application to NMR, 121
overview, 120–121
proposed mode of interaction with MPs, 121
structure, 121
trapping of PagP, 121
Lipopolysaccharide (LPS)
chemical structure, 3
effect on organization of OmpF in APol-depleted
samples, 557, 560
role in OmpA renaturation, 334
Liposomes, 133
Living radical polymerization, 220
Local and general anaesthetics
action on the nAChR, 37
Loll, P.J., 104
Low-density lipoprotein (LDL)
receptors, 12
LPS, see Lipopolysaccharide (LPS)
Lysosomes, 6, 12
role of saposins, 123
M
Major outer membrane protein (MOMP) from
C. trachomatis
absence of protection by recombinant MOMP, 664,
666–668
CD spectrum, 665
NMR detection of tryptophan side chains in A8-35
vs. detergent, 668
NMR studies of MOMP/A8-35 complexes, 488
proposed structure, 664
resistance to freeze/thawing in A8-35 vs. detergent,
665, 666
shelf life in A8-35 vs. detergent, 665, 666
stabilization by A8-35, 664, 665
stabilization by APols, 294
thermal denaturation followed by CD spectroscopy,
392, 393
thermal stability in A8-35 vs. detergent solution,
392, 393
topological model, 664
vaccination attempts using native MOMP, 664
MALDI, see Matrix-assisted laser desorption/ionization
(MALDI)
MalF, 263
MalG, 263
MalK, 263
Maltose-neopentyl glycol (MNG) amphiphiles
detergency, 135
Maltose-neopentyl glycol (MNG) detergents, 83
Maltose transporter, 261, 263
Mannitol permease
activity in A8-35, 289
fluorescence quenching by FAPol fluo , 397, 399
FRET with FAPol fluo , 397, 399
Martinez, K.L., 601
Mass spectrometry of MP/APols complexes
comparison of various APols, 639
comparison with DDM, 639
ESI-MS studies of monomeric MPs, 636–644
ESI-MS studies of oligomeric MPs, 640
MALDI-TOF studies, 633–636
can MS-optimized APols be designed?, 643–644
overview, 632
Mass spectrometry of MPs from detergent solutions, 628
correlation between detergent stripping and
denaturation, 628
detergent stripping, 627–630
MD simulation of transfer to vacuum, 631
stability of MP/detergent complexes in vacuo, 630
Matrix-assisted laser desorption/ionization (MALDI), 626
principle, 626
M13 coat protein
conformational thermostabilization, 84
Mechanosensitive channel, large pore (E. coli) (MscL), 10
CFE in A8-35, 366, 369
CFE in SAPols, 366, 369
TM structure, 17
Melatonin MT1R receptor, 288, 289
Melittin, 118
Membrane fragments
structural and functional studies, 60
Membrane protein/A8-35 complexes
effect of EDTA on rotational correlation time,
422–423
overestimation of their size by SEC, 435
Membrane protein/APol complexes, 462
accessibility to the solvent mapped by NMR, 471, 472
analysis by SGSV, 411
APol binding, 273, 274
AUC analysis, 423, 440–443
BAPol-mediated immobilization with multiplexing,
600, 601, 603
BAPol-mediated micro-patterning, 600, 601, 603
BN-PAGE analysis, 419
CD and SRCD, 391–392
comparison with MP/DDM complexes, 273, 281
composition, organisation, dynamics, and solution
properties, 266–286
crystallization, 508–523
attempts in aqueous solution, 509–516
as ternary MP/APol/detergent complexes, 509–516
by transfer to lipidic mesophases, 516–522
determination of rotational correlation times by
solution NMR, 422–423
DLS studies, 438–439
dynamics, 296–305
effects of removing free APols, 257, 418, 419
ESI-MS analyses of monomeric MPs, 636–640
ESI-MS analyses of oligomeric MPs, 640
698
Index
Lipids, 2, 3
amphipathic character, 2
chemical structure of some polar lipids, 3
self-association in water, 4
Lipodisqs
®
, 164
See also styrenealeic acid co-polymers
Lipopeptide detergents, 118, 119, 134
application to NMR, 121
overview, 120–121
proposed mode of interaction with MPs, 121
structure, 121
trapping of PagP, 121
Lipopolysaccharide (LPS)
chemical structure, 3
effect on organization of OmpF in APol-depleted
samples, 557, 560
role in OmpA renaturation, 334
Liposomes, 133
Living radical polymerization, 220
Local and general anaesthetics
action on the nAChR, 37
Loll, P.J., 104
Low-density lipoprotein (LDL)
receptors, 12
LPS, see Lipopolysaccharide (LPS)
Lysosomes, 6, 12
role of saposins, 123
M
Major outer membrane protein (MOMP) from
C. trachomatis
absence of protection by recombinant MOMP, 664,
666–668
CD spectrum, 665
NMR detection of tryptophan side chains in A8-35
vs. detergent, 668
NMR studies of MOMP/A8-35 complexes, 488
proposed structure, 664
resistance to freeze/thawing in A8-35 vs. detergent,
665, 666
shelf life in A8-35 vs. detergent, 665, 666
stabilization by A8-35, 664, 665
stabilization by APols, 294
thermal denaturation followed by CD spectroscopy,
392, 393
thermal stability in A8-35 vs. detergent solution,
392, 393
topological model, 664
vaccination attempts using native MOMP, 664
MALDI, see Matrix-assisted laser desorption/ionization
(MALDI)
MalF, 263
MalG, 263
MalK, 263
Maltose-neopentyl glycol (MNG) amphiphiles
detergency, 135
Maltose-neopentyl glycol (MNG) detergents, 83
Maltose transporter, 261, 263
Mannitol permease
activity in A8-35, 289
fluorescence quenching by FAPol fluo , 397, 399
FRET with FAPol fluo , 397, 399
Martinez, K.L., 601
Mass spectrometry of MP/APols complexes
comparison of various APols, 639
comparison with DDM, 639
ESI-MS studies of monomeric MPs, 636–644
ESI-MS studies of oligomeric MPs, 640
MALDI-TOF studies, 633–636
can MS-optimized APols be designed?, 643–644
overview, 632
Mass spectrometry of MPs from detergent solutions, 628
correlation between detergent stripping and
denaturation, 628
detergent stripping, 627–630
MD simulation of transfer to vacuum, 631
stability of MP/detergent complexes in vacuo, 630
Matrix-assisted laser desorption/ionization (MALDI), 626
principle, 626
M13 coat protein
conformational thermostabilization, 84
Mechanosensitive channel, large pore (E. coli) (MscL), 10
CFE in A8-35, 366, 369
CFE in SAPols, 366, 369
TM structure, 17
Melatonin MT1R receptor, 288, 289
Melittin, 118
Membrane fragments
structural and functional studies, 60
Membrane protein/A8-35 complexes
effect of EDTA on rotational correlation time,
422–423
overestimation of their size by SEC, 435
Membrane protein/APol complexes, 462
accessibility to the solvent mapped by NMR, 471, 472
analysis by SGSV, 411
APol binding, 273, 274
AUC analysis, 423, 440–443
BAPol-mediated immobilization with multiplexing,
600, 601, 603
BAPol-mediated micro-patterning, 600, 601, 603
BN-PAGE analysis, 419
CD and SRCD, 391–392
comparison with MP/DDM complexes, 273, 281
composition, organisation, dynamics, and solution
properties, 266–286
crystallization, 508–523
attempts in aqueous solution, 509–516
as ternary MP/APol/detergent complexes, 509–516
by transfer to lipidic mesophases, 516–522
determination of rotational correlation times by
solution NMR, 422–423
DLS studies, 438–439
dynamics, 296–305
effects of removing free APols, 257, 418, 419
ESI-MS analyses of monomeric MPs, 636–640
ESI-MS analyses of oligomeric MPs, 640
698
Index