Ergosterol, 6
chemical structure, 3
ESI, see Electrospray ionization (ESI)
ESI-IM-MS, see Electrospray ionization-ion mobilitymass spectrometry (ESI-IM-MS)
ExbB 4 /ExbD 2 /A8-35 complexes
AF4-MALLS analysis, 419, 421
SAXS, SANS and EM studies, 437–438
SEC analysis, 419, 421
ExbB 4 /ExbD 2 complexes, 274, 280
APol binding, 274
NS SP EM studies, 559
ExbB 6 -ExbD 4 complex
NS SP EM studies, 559
F
Facial amphiphiles, 82
Faham, S., 104
Fast photochemical oxidation of proteins (FPOP),
283, 641
flow-chart, 641
Fat pads
trapping of APols injected to mice, 677
FC12, see Fos-Choline-12
F 6 -DiGlu, 130
Ferric enterobactin receptor (FepA)
3D structure, 21
TM structure, 18
F 1 F O ATP-synthase, 10
disaggregation by A8-35, 294
inhibition by A8-35, 290
SP NS study of A8-75-trapped ATPase, 549
FGFR3 receptor
mutation-induced dimerization, 17
FGK 2 complex, see Maltose transporter
F 6 H 3 DigluM
chemical structure, 129
FhuA, 21, 289
Flagella, bacterial, 11
Fluid mosaic model, 22, 24
Fluorinated surfactants, 134
applications, 131–132
background, 128–129
chemical structures, 129
F 4 H 5 βM, 129
F 6 H 3 DigluM, 129
F4-MNG, 129
F12-MNG, 129
F 6 OM, 130, 132
F 6 OPC, 130, 132
fluorinated amidosulfobetaines, 130
fluorinated octylmaltoside, 132
F-TAC, 129
H 3 F 6 H 3 DigluM, 129, 130
HF-MNG, 130
HF-TAC, 129
overview, 128–132
perfluoro-octanoate, 128, 132
Folding MPs in APols, 265–266
challenges and prospects, 353–354
disulfide bridges, 350
folding of oligomeric MPs, 353
hypothetical mechanisms, 352
importance of the unfolded state, 350
Protocol 6.1, 354–356
role of lipids, 350
why is a membrane environment not needed?, 353
why is it so efficient?, 350–353
Folding MPs in vitro
APol-assisted folding, 338, 340
classical aproaches, 334–337
overview, 336, 337
FomA (F. nucleatum)
APol-assisted folding, 340, 347–348
CD spectra before and after folding in A8-35
vs. LDAO, 391
transfer from A8-35 to black lipid films, 308, 309
Förster resonance energy transfer (FRET)
between mannitol permease and FAPol fluo , 397
between tOmpA and FAPol NBD , 298
Fos-choline-12, see Dodecylphosphocholine
Fourier shell correlation (FSC), 537
use for estimating the resolution of EM
reconstructions, 537, 538
Fourier transform, 537
Fourier transform infra-red (FTIR) spectroscopy
of SMA, 394
FPOP, see Fast photochemical oxidation of proteins
(FPOP)
Free-electron laser X-ray (X-FEL) crystallography, 499
application to MPs, 499
FRET, see Förster resonance energy transfer
(FRET)
FSC, see Fourier shell correlation (FSC)
F-TAC, 130
chemical structure, 129
FTIR spectroscopy, see Fourier transform infra-red (FTIR)
spectroscopy
Functional studies of APol-trapped MPs, 286
Functionalizing polymers
synthesis routes, 225–227
G
GABA A receptor, 39
Galactose permease (GalP)
ESI-MS analyses of GalP/A8-35 complexes, 638
Gangliosides
chemical structure, 3
Gap junctions, 10, 12
Gautier, A., 458
Gellman, S.H., 83
Ghrelin GHS-R1a receptor, 287, 289
APol-assisted folding, 340
folding in NAPols, 345
Giusti, F., 161, 210, 227, 394, 400, 467
GLIC, 38–41
Index
695
chemical structure, 3
ESI, see Electrospray ionization (ESI)
ESI-IM-MS, see Electrospray ionization-ion mobilitymass spectrometry (ESI-IM-MS)
ExbB 4 /ExbD 2 /A8-35 complexes
AF4-MALLS analysis, 419, 421
SAXS, SANS and EM studies, 437–438
SEC analysis, 419, 421
ExbB 4 /ExbD 2 complexes, 274, 280
APol binding, 274
NS SP EM studies, 559
ExbB 6 -ExbD 4 complex
NS SP EM studies, 559
F
Facial amphiphiles, 82
Faham, S., 104
Fast photochemical oxidation of proteins (FPOP),
283, 641
flow-chart, 641
Fat pads
trapping of APols injected to mice, 677
FC12, see Fos-Choline-12
F 6 -DiGlu, 130
Ferric enterobactin receptor (FepA)
3D structure, 21
TM structure, 18
F 1 F O ATP-synthase, 10
disaggregation by A8-35, 294
inhibition by A8-35, 290
SP NS study of A8-75-trapped ATPase, 549
FGFR3 receptor
mutation-induced dimerization, 17
FGK 2 complex, see Maltose transporter
F 6 H 3 DigluM
chemical structure, 129
FhuA, 21, 289
Flagella, bacterial, 11
Fluid mosaic model, 22, 24
Fluorinated surfactants, 134
applications, 131–132
background, 128–129
chemical structures, 129
F 4 H 5 βM, 129
F 6 H 3 DigluM, 129
F4-MNG, 129
F12-MNG, 129
F 6 OM, 130, 132
F 6 OPC, 130, 132
fluorinated amidosulfobetaines, 130
fluorinated octylmaltoside, 132
F-TAC, 129
H 3 F 6 H 3 DigluM, 129, 130
HF-MNG, 130
HF-TAC, 129
overview, 128–132
perfluoro-octanoate, 128, 132
Folding MPs in APols, 265–266
challenges and prospects, 353–354
disulfide bridges, 350
folding of oligomeric MPs, 353
hypothetical mechanisms, 352
importance of the unfolded state, 350
Protocol 6.1, 354–356
role of lipids, 350
why is a membrane environment not needed?, 353
why is it so efficient?, 350–353
Folding MPs in vitro
APol-assisted folding, 338, 340
classical aproaches, 334–337
overview, 336, 337
FomA (F. nucleatum)
APol-assisted folding, 340, 347–348
CD spectra before and after folding in A8-35
vs. LDAO, 391
transfer from A8-35 to black lipid films, 308, 309
Förster resonance energy transfer (FRET)
between mannitol permease and FAPol fluo , 397
between tOmpA and FAPol NBD , 298
Fos-choline-12, see Dodecylphosphocholine
Fourier shell correlation (FSC), 537
use for estimating the resolution of EM
reconstructions, 537, 538
Fourier transform, 537
Fourier transform infra-red (FTIR) spectroscopy
of SMA, 394
FPOP, see Fast photochemical oxidation of proteins
(FPOP)
Free-electron laser X-ray (X-FEL) crystallography, 499
application to MPs, 499
FRET, see Förster resonance energy transfer
(FRET)
FSC, see Fourier shell correlation (FSC)
F-TAC, 130
chemical structure, 129
FTIR spectroscopy, see Fourier transform infra-red (FTIR)
spectroscopy
Functional studies of APol-trapped MPs, 286
Functionalizing polymers
synthesis routes, 225–227
G
GABA A receptor, 39
Galactose permease (GalP)
ESI-MS analyses of GalP/A8-35 complexes, 638
Gangliosides
chemical structure, 3
Gap junctions, 10, 12
Gautier, A., 458
Gellman, S.H., 83
Ghrelin GHS-R1a receptor, 287, 289
APol-assisted folding, 340
folding in NAPols, 345
Giusti, F., 161, 210, 227, 394, 400, 467
GLIC, 38–41
Index
695