Detergents (cont.)
polyoxyethylene-based, 62
residency time in micelles, 65
selective extraction of MPs, 67
solubilization of fats, 60
DHPC, see Dihexanoyl- or
diheptanoylphosphatidylcholine
Dhurrin
metabolon, 261
synthesis, 261
Diacylglycerol kinase (DAGK), 258, 289, 308
in bicelles, 100
conformational thermostabilization, 84
ESI-MS study of DAGK/A8-35 complexes, 640
ESI-MS study of DAGK/APol complexes, 641
DIBMA, see Diisobutylene-maleic acid co-polymer
(DIBMA)
diC 6 PC, see Dihexanoylphosphatidylcholine
diC 7 PC, see Diheptanoylphosphatidylcholine
Dicyclohexylurea (DCU), 266, 281
artefactual grafting onto A8-35, 182
Dielectric constant (ε)
in water and inside lipid bilayers, 8
Digitonin, 62, 134
chemical structure, 61
removal using γ-cyclodextrin, 256
Diheptanoylphosphatidylcholine (diC 7 PC, DHPC), 61
chemical structure, 61
CMC, 61
detergent-like behavior, 62
Dihexanoylphosphatidylcholine (diC 6 PC, DHPC), 82
chemical structure, 61
CMC, 61
detergent-like behavior, 62
Diisobutylene-maleic acid co-polymer (DIBMA),
164, 261
UV spectrum, 382
Diphteria toxin, 308
Direct extraction of MPs, 259
Direct extraction of MPs by A8-35, 261
insulin receptor, 261
maltose transporter, 263
Direct extraction of MPs by SMA, 260
Direct extraction of proteins from membranes, 259
Displacement of MP-bound APols by other
surfactants, 306
Distribution of A8-35 following injection in mice
intraperitoneal injection, 675, 676, 678
intravenous injection, 675, 678
subcutaneous injection, 676–678
Disulfide bond formation protein B (DsbB)
made water-soluble by fusion to an ApoA-I
fragment, 109
Di-β-D-maltoside cholane, 82
chemical structure, 83
DLS, see Dynamic light scattering (DLS)
Dodecylmaltoside (DDM), 62, 82
chemical structure, 61
CMC, 61
detergency, 135
phase diagram, 76, 77
residency time in micelles, 65
Dodecylphosphocholine (DPC), 82
chemical structure, 61
CMC, 61
MD simulation of micelle formation, 64
Dodecylsulfate (DS)
precipitation with KCl, 339
Double-electron-electron resonance (DEER)
application to SMA-trapped BR, 421
DPC, see Dodecylphosphocholine (DPC)
DS, see Dodecylsulfate (DS)
DsbB, see Disulfide bond formation protein B (DsbB)
DST, see Dynamic surface tension
D t , see Translational diffusion coefficient
Dynactin
SP cryo-EM analysis, 572
Dynamic light scattering (DLS), 438–439
E
Ebel, C., 443
EBs, see Elementary bodies (C. trachomatis)
Effects of APols on MP dynamics, stability and function,
296–305
EII
mtl mannitol permease
activity in A8-35, 289
fluorescence quenching by FAPol fluo , 397, 399
FRET with FAPol fluo , 397, 399
EINS, see Elastic incoherent neutron scattering (EINS)
Elastic incoherent neutron scattering (EINS)
by A8-35 particles, 187, 188
Electron microscopy of MPs
approaches, 534
overview, 534–541
Electron resonance spectroscopy, 421
Electron tomography, 534
Electrospray ionization (ESI)
principle, 626
Electrospray ionization-ion mobility-mass spectrometry
(ESI-IM-MS)
of A8-35, 177
application to MP/APol complexes, 650
of perDAPol, 177
principle, 638
protocol for application to MP/APol samples (Protocol
14.1), 648–654
ELIC, 39
EM, see Electron microscopy
Endoplasmic reticulum (ER), 6, 12, 48
Endosomes, 12
Engelman, D.M., 48, 50
Equilibrative nucleoside transporter-1, 277
lipid binding in SMALPs, 278
Equilibrium (isopycnic) sedimentation in sucrose
gradients
principle, 412
Equilibrium sedimentation analytical ultracentrifugation
(Eq-AUC)
principle, 412
ER, see Endoplasmic reticulum (ER)
694
Index
polyoxyethylene-based, 62
residency time in micelles, 65
selective extraction of MPs, 67
solubilization of fats, 60
DHPC, see Dihexanoyl- or
diheptanoylphosphatidylcholine
Dhurrin
metabolon, 261
synthesis, 261
Diacylglycerol kinase (DAGK), 258, 289, 308
in bicelles, 100
conformational thermostabilization, 84
ESI-MS study of DAGK/A8-35 complexes, 640
ESI-MS study of DAGK/APol complexes, 641
DIBMA, see Diisobutylene-maleic acid co-polymer
(DIBMA)
diC 6 PC, see Dihexanoylphosphatidylcholine
diC 7 PC, see Diheptanoylphosphatidylcholine
Dicyclohexylurea (DCU), 266, 281
artefactual grafting onto A8-35, 182
Dielectric constant (ε)
in water and inside lipid bilayers, 8
Digitonin, 62, 134
chemical structure, 61
removal using γ-cyclodextrin, 256
Diheptanoylphosphatidylcholine (diC 7 PC, DHPC), 61
chemical structure, 61
CMC, 61
detergent-like behavior, 62
Dihexanoylphosphatidylcholine (diC 6 PC, DHPC), 82
chemical structure, 61
CMC, 61
detergent-like behavior, 62
Diisobutylene-maleic acid co-polymer (DIBMA),
164, 261
UV spectrum, 382
Diphteria toxin, 308
Direct extraction of MPs, 259
Direct extraction of MPs by A8-35, 261
insulin receptor, 261
maltose transporter, 263
Direct extraction of MPs by SMA, 260
Direct extraction of proteins from membranes, 259
Displacement of MP-bound APols by other
surfactants, 306
Distribution of A8-35 following injection in mice
intraperitoneal injection, 675, 676, 678
intravenous injection, 675, 678
subcutaneous injection, 676–678
Disulfide bond formation protein B (DsbB)
made water-soluble by fusion to an ApoA-I
fragment, 109
Di-β-D-maltoside cholane, 82
chemical structure, 83
DLS, see Dynamic light scattering (DLS)
Dodecylmaltoside (DDM), 62, 82
chemical structure, 61
CMC, 61
detergency, 135
phase diagram, 76, 77
residency time in micelles, 65
Dodecylphosphocholine (DPC), 82
chemical structure, 61
CMC, 61
MD simulation of micelle formation, 64
Dodecylsulfate (DS)
precipitation with KCl, 339
Double-electron-electron resonance (DEER)
application to SMA-trapped BR, 421
DPC, see Dodecylphosphocholine (DPC)
DS, see Dodecylsulfate (DS)
DsbB, see Disulfide bond formation protein B (DsbB)
DST, see Dynamic surface tension
D t , see Translational diffusion coefficient
Dynactin
SP cryo-EM analysis, 572
Dynamic light scattering (DLS), 438–439
E
Ebel, C., 443
EBs, see Elementary bodies (C. trachomatis)
Effects of APols on MP dynamics, stability and function,
296–305
EII
mtl mannitol permease
activity in A8-35, 289
fluorescence quenching by FAPol fluo , 397, 399
FRET with FAPol fluo , 397, 399
EINS, see Elastic incoherent neutron scattering (EINS)
Elastic incoherent neutron scattering (EINS)
by A8-35 particles, 187, 188
Electron microscopy of MPs
approaches, 534
overview, 534–541
Electron resonance spectroscopy, 421
Electron tomography, 534
Electrospray ionization (ESI)
principle, 626
Electrospray ionization-ion mobility-mass spectrometry
(ESI-IM-MS)
of A8-35, 177
application to MP/APol complexes, 650
of perDAPol, 177
principle, 638
protocol for application to MP/APol samples (Protocol
14.1), 648–654
ELIC, 39
EM, see Electron microscopy
Endoplasmic reticulum (ER), 6, 12, 48
Endosomes, 12
Engelman, D.M., 48, 50
Equilibrative nucleoside transporter-1, 277
lipid binding in SMALPs, 278
Equilibrium (isopycnic) sedimentation in sucrose
gradients
principle, 412
Equilibrium sedimentation analytical ultracentrifugation
(Eq-AUC)
principle, 412
ER, see Endoplasmic reticulum (ER)
694
Index