CHAPSO, 62
chemical structure, 61
CMC, 61
micelle structure, 65
Chemistry of synthetic polymers
an introduction, 161
Chemoreceptors
ND-trapped, 116
Cheng, Y., 564
Chlamydia muridarum, 662
Chlamydia trachomatis, 294, 661, 662
developing an APol-based vaccine, 664–669
elementary bodies (EBs), 662
epidemiology, 662
life cycle, 662, 663
major outer membrane protein (MOMP), 664
pathology, 662
serovars, 664
subunit vaccines, 664
treatments, 662
vaccination attempts, 662
using MOMP/A8-35 preparations, 667, 668
using MOMP/EP67-A8-35 preparations, 670
Chlamydomonas reinhardtii, 261
cytochrome b 6 f complex, 72
Chloroplasts, 4
lumen, 6
sacculae, 6
Cholate, 62
chemical structure, 61
CMC, 61
micelle structure, 65
Cholesterol, 6, 43
chemical structure, 3
role in nAChR coupling, 43
specifically bound to β 2 AR, 28
stabilizing effect on GPCRs, 78
Cholesterol hemisuccinate
stabilizing effect on GPCRs, 78
Cholesteryl esters, 107
CID, see Collision-induced dissociation (CID)
Circular dichroism (CD)
of APol-trapped MPs, 391–392
CMC, see Critical micellar concentration (CMC)
C m E n detergents, 62
CMP, see Contrast-match point
Cocco, M.J., 483, 664
Collision cross-section (CCS), 177, 627
principle, 627
Collision-induced dissociation (CID)
principle, 627
Complex I
as part of the respirasome, 562
SP cryo-EM study in A8-35, 550, 552, 553
3D structure, 21, 23
Complex III, see Cytochrome bc 1 complex
Complex IV, see Cytochrome c oxidase
Complex V, see F 1 F O ATP-synthase
Composition of MP-bound surfactant belts in APol/
detergent mixtures, 298
Conical tilt reconstruction, 575
Connexon, 10, 12
Contrast-matching in SANS and neutron
crystallography, 428
Contrast-match point, 428
of lipids, 430
of partially deuterated A8-35 (DAPol), 428, 430
of perdeuterated A8-35 (perDAPol), 428, 430
of proteins, 430
of unlabeled A8-35 (HAPol), 428, 430
Controlled/living radical polymerization (CRP), 219–222
atom transfer radical polymerization (ATRP), 219
nitroxide-mediated polymerization (NMP), 219
overview, 221
reversible addition-fragmentation chain transfer
polymerization (RAFT), 219
Controlling polymer mass and dispersity
controlled/living radical polymerization (CRP),
219–222
telomerization (chain transfer), 218–219
Corringer, P.-J., 38
Crémel, G., 261
CRFR1, 282, 289
CFE in NVoy, 367, 370, 371
CRFR2β, 282, 289, 294
CFE in NVoy, 367, 370, 371
2D NMR spectrum in NVoy, 479
CRFR2β/NVoy complexes
SEC studies, 420
Critical association concentration (CAC)
of A8-35, 170–176
of APG, 175
of lipids, 4
Critical micellar concentration (CMC), 63
definition, 62
factors determining it, 63
Cryo-EM, see Electron cryomicroscopy
Crystallization of MP/APol complexes
in aqueous solution, 509–516
in meso, 516–522
overview, 510
protocol for crystallizing ternary MP/APol/detergent
complexes (Protocol 11.1), 523–524
as ternary MP/APol/detergent complexes, 509–516
Crystallization of MPs, 497–508
in cubo, 507
from detergent solutions, 503–506
in meso, 507
CsgG/CsgE complex
SP cryo-EM reconstruction in A8-35, 569
Curare, 37
Cyclic-nucleotide-gated channel
SP cryo-EM reconstruction in A8-35, 569
Cymal series, 82
Cytochrome b 6 , 73
Cytochrome bc 1 /A8-35 complexes
aggregation in the absence of free APol, 512, 513
composition, 510
crystallization as ternary MP/APol/detergent
complexes, 509–516
692
Index
chemical structure, 61
CMC, 61
micelle structure, 65
Chemistry of synthetic polymers
an introduction, 161
Chemoreceptors
ND-trapped, 116
Cheng, Y., 564
Chlamydia muridarum, 662
Chlamydia trachomatis, 294, 661, 662
developing an APol-based vaccine, 664–669
elementary bodies (EBs), 662
epidemiology, 662
life cycle, 662, 663
major outer membrane protein (MOMP), 664
pathology, 662
serovars, 664
subunit vaccines, 664
treatments, 662
vaccination attempts, 662
using MOMP/A8-35 preparations, 667, 668
using MOMP/EP67-A8-35 preparations, 670
Chlamydomonas reinhardtii, 261
cytochrome b 6 f complex, 72
Chloroplasts, 4
lumen, 6
sacculae, 6
Cholate, 62
chemical structure, 61
CMC, 61
micelle structure, 65
Cholesterol, 6, 43
chemical structure, 3
role in nAChR coupling, 43
specifically bound to β 2 AR, 28
stabilizing effect on GPCRs, 78
Cholesterol hemisuccinate
stabilizing effect on GPCRs, 78
Cholesteryl esters, 107
CID, see Collision-induced dissociation (CID)
Circular dichroism (CD)
of APol-trapped MPs, 391–392
CMC, see Critical micellar concentration (CMC)
C m E n detergents, 62
CMP, see Contrast-match point
Cocco, M.J., 483, 664
Collision cross-section (CCS), 177, 627
principle, 627
Collision-induced dissociation (CID)
principle, 627
Complex I
as part of the respirasome, 562
SP cryo-EM study in A8-35, 550, 552, 553
3D structure, 21, 23
Complex III, see Cytochrome bc 1 complex
Complex IV, see Cytochrome c oxidase
Complex V, see F 1 F O ATP-synthase
Composition of MP-bound surfactant belts in APol/
detergent mixtures, 298
Conical tilt reconstruction, 575
Connexon, 10, 12
Contrast-matching in SANS and neutron
crystallography, 428
Contrast-match point, 428
of lipids, 430
of partially deuterated A8-35 (DAPol), 428, 430
of perdeuterated A8-35 (perDAPol), 428, 430
of proteins, 430
of unlabeled A8-35 (HAPol), 428, 430
Controlled/living radical polymerization (CRP), 219–222
atom transfer radical polymerization (ATRP), 219
nitroxide-mediated polymerization (NMP), 219
overview, 221
reversible addition-fragmentation chain transfer
polymerization (RAFT), 219
Controlling polymer mass and dispersity
controlled/living radical polymerization (CRP),
219–222
telomerization (chain transfer), 218–219
Corringer, P.-J., 38
Crémel, G., 261
CRFR1, 282, 289
CFE in NVoy, 367, 370, 371
CRFR2β, 282, 289, 294
CFE in NVoy, 367, 370, 371
2D NMR spectrum in NVoy, 479
CRFR2β/NVoy complexes
SEC studies, 420
Critical association concentration (CAC)
of A8-35, 170–176
of APG, 175
of lipids, 4
Critical micellar concentration (CMC), 63
definition, 62
factors determining it, 63
Cryo-EM, see Electron cryomicroscopy
Crystallization of MP/APol complexes
in aqueous solution, 509–516
in meso, 516–522
overview, 510
protocol for crystallizing ternary MP/APol/detergent
complexes (Protocol 11.1), 523–524
as ternary MP/APol/detergent complexes, 509–516
Crystallization of MPs, 497–508
in cubo, 507
from detergent solutions, 503–506
in meso, 507
CsgG/CsgE complex
SP cryo-EM reconstruction in A8-35, 569
Curare, 37
Cyclic-nucleotide-gated channel
SP cryo-EM reconstruction in A8-35, 569
Cymal series, 82
Cytochrome b 6 , 73
Cytochrome bc 1 /A8-35 complexes
aggregation in the absence of free APol, 512, 513
composition, 510
crystallization as ternary MP/APol/detergent
complexes, 509–516
692
Index