A8-35 particles (cont.)
molecular mass, 181
polydispersity, 181
QENS analysis, 187–189
radius of gyration (R g ), 179, 180
SANS analysis, 178, 183, 184
SAXS analysis, 179
SEC analysis, 178, 180
second virial coefficient (A 2 ), 192
sedimentation coefficient (s 20,w ), 179, 181
sensitivity to divalent cations, 419, 420
shape, 179
shape and internal organization, 183–187
size and composition (overview), 180, 181
size, shape and organization, 176–192
SLS analysis, 180
solution behavior, 178
solution properties (overview), 180, 181
Stokes radius (R S ), 179, 180
Stuhrmann plots, 181
SV-AUC analysis, 178–180
thermal dynamics, 187
A8-75, 249, 252, 295
chemical structure, 158, 159
14
C-labeled, 414
naphthalene-labeled, 198, 397–399
solution properties, 192
A8-75 derivatives
azobenzene-tagged, 198
isotopically labeled, 198, 202
overview, 198–204
synthesis, 198
A34-35
chemical structure, 159
A34-75
chemical structure, 159
A 2A R, see Adenosine A 2A receptor (A 2A R)
ABCA4
SP NS EM study in A8-35, 557
ABC transporters
ABCA4, 289, 557
ABCB1 ¼ P-glycoprotein, 569
maltose transporter, 263
MsbA, 119
nucleotide binding in SMALPs, 289
Accessibility of TM surfaces in APol-trapped MPs
explored by FPOP coupled to LC and MS, 283
1
H/
2
H exchange kinetics, 283
probed by MD simulations, 285
sensitivity to a water-soluble paramagnetic
agent, 283
Acetylcholine (ACh), 10, 36
Acetylcholine-binding protein, 39
ACh, see Acetylcholine (ACh)
AcrAB-TolC complex
SP cryo-EM reconstruction in A8-35, 569
AcrABZ-TolC
SP cryo-EM reconstruction in A8-35, 569
AcrB, 260, 277, 283
SMA-trapped, 282
SP NS EM study of AcrB/SMA complexes, 559, 562
SV-AUC analysis of AcrB/SMA complexes, 425, 427
Action potential, 8
Acyl chains
C m:n notation, 3
Adenosine A 2A receptor (A 2A R), 289
conformational thermostabilization, 87
stability in SMALPs, 294, 295
Adrenergic receptor, β 1 - (β 1 AR)
conformational thermostabilization, 87
Adrenergic receptor, β 2 - (β 2 AR)
crystallization from bicelles, 104
signal transduction, 85, 86
specific binding of cholesterol, 28
AF4-MALLS, see Asymmetric flow field-flow
fractionation multi-angle laser light scattering
AFM, see Atomic force microscopy
Aggregation of MP/APol complexes
upon removal of free APol, 257, 258
as a way to form concentrated samples, 291
Air-water interface, 2
Alternatives to detergents
amphipathic peptides, 118
amphipols, 151–152
bicelles, 99–105
compared properties, 133–135
fluorinated surfactants, 128–132
nanodiscs, 105–118
overview, 98, 133–135
saposin A, 123–127
Amphibiopols, 169
Amphipathic molecules, 2
Amphipathic peptides
designer peptide surfactants (peptergents), 121–123
lipopeptide detergents, 120–121
melittin, 118
overview, 118, 119
peptitergents, 120
saposin A, 123–127
Amphipathic polymers, 152
Amphipol-assisted cell-free synthesis of MPs, 266
Amphipol-assisted folding of MPs, 338, 340
by adsorption of dodecylsulfate onto polystyrene
beads, 343
of α-helical MPs, 339–346
bacterio-opsin, 343
bacteriorhodopsin, 339–343
of β-barrel MPs, 346–350
cannabinoid CB1 receptor, 340, 345
by dodecylsulfate dialysis, 343
by dodecylsulfate precipitation, 341
effect of lipids, 342
folding in NAPols, 345–346
FomA (F. nucleatum), 340, 347–348
ghrelin GHS-R1a receptor, 340
GLUT4, 340
688
Index
molecular mass, 181
polydispersity, 181
QENS analysis, 187–189
radius of gyration (R g ), 179, 180
SANS analysis, 178, 183, 184
SAXS analysis, 179
SEC analysis, 178, 180
second virial coefficient (A 2 ), 192
sedimentation coefficient (s 20,w ), 179, 181
sensitivity to divalent cations, 419, 420
shape, 179
shape and internal organization, 183–187
size and composition (overview), 180, 181
size, shape and organization, 176–192
SLS analysis, 180
solution behavior, 178
solution properties (overview), 180, 181
Stokes radius (R S ), 179, 180
Stuhrmann plots, 181
SV-AUC analysis, 178–180
thermal dynamics, 187
A8-75, 249, 252, 295
chemical structure, 158, 159
14
C-labeled, 414
naphthalene-labeled, 198, 397–399
solution properties, 192
A8-75 derivatives
azobenzene-tagged, 198
isotopically labeled, 198, 202
overview, 198–204
synthesis, 198
A34-35
chemical structure, 159
A34-75
chemical structure, 159
A 2A R, see Adenosine A 2A receptor (A 2A R)
ABCA4
SP NS EM study in A8-35, 557
ABC transporters
ABCA4, 289, 557
ABCB1 ¼ P-glycoprotein, 569
maltose transporter, 263
MsbA, 119
nucleotide binding in SMALPs, 289
Accessibility of TM surfaces in APol-trapped MPs
explored by FPOP coupled to LC and MS, 283
1
H/
2
H exchange kinetics, 283
probed by MD simulations, 285
sensitivity to a water-soluble paramagnetic
agent, 283
Acetylcholine (ACh), 10, 36
Acetylcholine-binding protein, 39
ACh, see Acetylcholine (ACh)
AcrAB-TolC complex
SP cryo-EM reconstruction in A8-35, 569
AcrABZ-TolC
SP cryo-EM reconstruction in A8-35, 569
AcrB, 260, 277, 283
SMA-trapped, 282
SP NS EM study of AcrB/SMA complexes, 559, 562
SV-AUC analysis of AcrB/SMA complexes, 425, 427
Action potential, 8
Acyl chains
C m:n notation, 3
Adenosine A 2A receptor (A 2A R), 289
conformational thermostabilization, 87
stability in SMALPs, 294, 295
Adrenergic receptor, β 1 - (β 1 AR)
conformational thermostabilization, 87
Adrenergic receptor, β 2 - (β 2 AR)
crystallization from bicelles, 104
signal transduction, 85, 86
specific binding of cholesterol, 28
AF4-MALLS, see Asymmetric flow field-flow
fractionation multi-angle laser light scattering
AFM, see Atomic force microscopy
Aggregation of MP/APol complexes
upon removal of free APol, 257, 258
as a way to form concentrated samples, 291
Air-water interface, 2
Alternatives to detergents
amphipathic peptides, 118
amphipols, 151–152
bicelles, 99–105
compared properties, 133–135
fluorinated surfactants, 128–132
nanodiscs, 105–118
overview, 98, 133–135
saposin A, 123–127
Amphibiopols, 169
Amphipathic molecules, 2
Amphipathic peptides
designer peptide surfactants (peptergents), 121–123
lipopeptide detergents, 120–121
melittin, 118
overview, 118, 119
peptitergents, 120
saposin A, 123–127
Amphipathic polymers, 152
Amphipol-assisted cell-free synthesis of MPs, 266
Amphipol-assisted folding of MPs, 338, 340
by adsorption of dodecylsulfate onto polystyrene
beads, 343
of α-helical MPs, 339–346
bacterio-opsin, 343
bacteriorhodopsin, 339–343
of β-barrel MPs, 346–350
cannabinoid CB1 receptor, 340, 345
by dodecylsulfate dialysis, 343
by dodecylsulfate precipitation, 341
effect of lipids, 342
folding in NAPols, 345–346
FomA (F. nucleatum), 340, 347–348
ghrelin GHS-R1a receptor, 340
GLUT4, 340
688
Index