Index
A
A5-35
chemical structure, 160
A8-35, 249, 252
apparent specific volume, 181
artefactual grafting of dicyclohexylurea (DCU), 182
CAC, 191
chemical structure, 153, 158
contrast-match point, 428
deprotonation above pH 7, 192
DST measurements, 190, 191
ESI-IM-MS analyses, 637, 638
inhibition of MP cell-free expression (CFE), 368
labeling routes (overview), 202
mass and dispersity of individual molecules, 176–177
molecular dispersity (Ð), 159, 176
number-average molecular mass (hM n i) of sodium
salt, 176
partial specific volume, 181
phase diagram, 191–192
rate of adsorption at the air-water interface, 190, 191
scattering length density, 181
sensitivity to multivalent cations, 194
solution properties, 170–192
synthesis, 159
use in MALDI-TOF studies, 633
use in proteomics, 644–647
UV spectrum, 381
weight-average mass (hM w i) of sodium salt, 160, 176
A8-35 derivatives, 198
adjuvant-carrying APols, 670
BAPol (biotinylated), 198, 202, 595, 601, 606
DAPol (deuterated), 176, 198, 266, 430, 464, 468,
479–481
EP67-A8-35, 198, 670
FAPol A647 , 198
FAPol AF488 , 198
FAPol AF647 , 198, 397, 400, 601, 614, 674
FAPol fluo , 198, 397, 399
FAPol NBD , 174, 198, 256, 298, 308, 397, 400, 425,
427, 514, 515, 601, 673, 674
FAPol rhod , 174, 198, 397, 400, 674, 676, 678
FAPols (fluorescent), 176
overview, 199, 398, 399
synthesis, 202
[
3
H]A8-35, 257, 266, 282
HistAPol (polyhistidine-tagged), 198, 202, 607, 608
ImidAPol (random imidazole moieties), 198, 202, 203,
607, 611
isotopically labeled, 198, 202
MethylAPol (N-methylated), 198, 394
OligAPol (oligonucleotide-tagged), 198, 203, 397,
448, 607, 613, 614, 670
SEC analysis, 204
overview, 198–204
perDAPol (perdeuterated), 176, 198, 428, 430, 464,
479–482
ResiqAPol (resiquimod-tagged), 198, 670
solution behavior, 203
SpinAPol (4-carboxy-tempo-tagged), 198, 401
SulfidAPol (thiomorpholine-tagged), 198, 400, 607,
615, 616
synthesis, 198
tagged (overview), 198
UAPol-NH 2 (synthesis intermediate), 201
UAPol (synthesis intermediate), 201
A8-35 particles
aggregation at pH 7, 192
aggregation induced by multivalent cations, 192
aggregation number, 181
associated water, 181
CAC, 170–176
determination by FRET measurements, 174
determination by surface tension
measurements, 175
DLS analysis, 178, 179
D t , from QENS measurements, 189, 191, 430
dynamics, 187–191
effect of divalent cations on dispersity, 183
effect of PEG on interparticle interactions, 192
EINS analysis, 187, 188
Eq-AUC analysis, 180
factors affecting the dispersity, 182
formation, structure and dynamics, 170–192
homogeneity, mass and size, 178–183
internal dynamics, 187, 188, 190
mass independent on pH and temperature, 192
MD simulations, 184, 185, 188, 190
# Springer International Publishing AG 2018
J. -L. Popot, Membrane Proteins in Aqueous Solutions, Biological and Medical Physics,
Biomedical Engineering, https://doi.org/10.1007/978-3-319-73148-3
687
A
A5-35
chemical structure, 160
A8-35, 249, 252
apparent specific volume, 181
artefactual grafting of dicyclohexylurea (DCU), 182
CAC, 191
chemical structure, 153, 158
contrast-match point, 428
deprotonation above pH 7, 192
DST measurements, 190, 191
ESI-IM-MS analyses, 637, 638
inhibition of MP cell-free expression (CFE), 368
labeling routes (overview), 202
mass and dispersity of individual molecules, 176–177
molecular dispersity (Ð), 159, 176
number-average molecular mass (hM n i) of sodium
salt, 176
partial specific volume, 181
phase diagram, 191–192
rate of adsorption at the air-water interface, 190, 191
scattering length density, 181
sensitivity to multivalent cations, 194
solution properties, 170–192
synthesis, 159
use in MALDI-TOF studies, 633
use in proteomics, 644–647
UV spectrum, 381
weight-average mass (hM w i) of sodium salt, 160, 176
A8-35 derivatives, 198
adjuvant-carrying APols, 670
BAPol (biotinylated), 198, 202, 595, 601, 606
DAPol (deuterated), 176, 198, 266, 430, 464, 468,
479–481
EP67-A8-35, 198, 670
FAPol A647 , 198
FAPol AF488 , 198
FAPol AF647 , 198, 397, 400, 601, 614, 674
FAPol fluo , 198, 397, 399
FAPol NBD , 174, 198, 256, 298, 308, 397, 400, 425,
427, 514, 515, 601, 673, 674
FAPol rhod , 174, 198, 397, 400, 674, 676, 678
FAPols (fluorescent), 176
overview, 199, 398, 399
synthesis, 202
[
3
H]A8-35, 257, 266, 282
HistAPol (polyhistidine-tagged), 198, 202, 607, 608
ImidAPol (random imidazole moieties), 198, 202, 203,
607, 611
isotopically labeled, 198, 202
MethylAPol (N-methylated), 198, 394
OligAPol (oligonucleotide-tagged), 198, 203, 397,
448, 607, 613, 614, 670
SEC analysis, 204
overview, 198–204
perDAPol (perdeuterated), 176, 198, 428, 430, 464,
479–482
ResiqAPol (resiquimod-tagged), 198, 670
solution behavior, 203
SpinAPol (4-carboxy-tempo-tagged), 198, 401
SulfidAPol (thiomorpholine-tagged), 198, 400, 607,
615, 616
synthesis, 198
tagged (overview), 198
UAPol-NH 2 (synthesis intermediate), 201
UAPol (synthesis intermediate), 201
A8-35 particles
aggregation at pH 7, 192
aggregation induced by multivalent cations, 192
aggregation number, 181
associated water, 181
CAC, 170–176
determination by FRET measurements, 174
determination by surface tension
measurements, 175
DLS analysis, 178, 179
D t , from QENS measurements, 189, 191, 430
dynamics, 187–191
effect of divalent cations on dispersity, 183
effect of PEG on interparticle interactions, 192
EINS analysis, 187, 188
Eq-AUC analysis, 180
factors affecting the dispersity, 182
formation, structure and dynamics, 170–192
homogeneity, mass and size, 178–183
internal dynamics, 187, 188, 190
mass independent on pH and temperature, 192
MD simulations, 184, 185, 188, 190
# Springer International Publishing AG 2018
J. -L. Popot, Membrane Proteins in Aqueous Solutions, Biological and Medical Physics,
Biomedical Engineering, https://doi.org/10.1007/978-3-319-73148-3
687