Table 8.1
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.39
No protein
SMA
The formation of SMALPs
using different weight ratios of
POPC/POPG lipids to SMA
polymers was characterized via
solid-state NMR and DLS
Zhang et al. (2015)
8.40
Melatonin MT1R and
ghrelin GHS-R1a GPCRs
SMA
●
CHS-R1a was expressed in
inclusion bodies, folded in
A8-35, and transferred to lipid
vesicles. MT1R was expressed
in Chinese hamster ovary
(CHO) cells, from which
membrane fragments were
purified. The two receptors
were extracted with SMA and
ligand binding studied by
fluorescence spectroscopy
Logez et al. (2016)
8.41
Mitochondrial respirasome
(I
1 III
2 IV
1 supercomplex)
A8-35
●
Comparative absorbance and
RRS spectra of mitochondrial
complexes in isolation and
integrated into a respiratory
supercomplex
Shinzawa-Itoh et al. (2016)
8.42
Yeast cytochrome
c
oxidase (Complex IV)
SMA
●
The binding or unbinding of
ligands (O
2 , CO) to Complex
IV was studied by TRAS
Smirnova et al. (2016)
8.43
No protein
SMA
●
Time-resolved FRET and
SANS were used to determine
the kinetics and mechanisms of
phospholipid transfer among
SMALPs
Cuevas Arenas et al. (2017)
(continued)
8.2 Optical Spectroscopy Studies of Amphipols and Membrane Protein/Amphipol Complexes
389
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.39
No protein
SMA
The formation of SMALPs
using different weight ratios of
POPC/POPG lipids to SMA
polymers was characterized via
solid-state NMR and DLS
Zhang et al. (2015)
8.40
Melatonin MT1R and
ghrelin GHS-R1a GPCRs
SMA
●
CHS-R1a was expressed in
inclusion bodies, folded in
A8-35, and transferred to lipid
vesicles. MT1R was expressed
in Chinese hamster ovary
(CHO) cells, from which
membrane fragments were
purified. The two receptors
were extracted with SMA and
ligand binding studied by
fluorescence spectroscopy
Logez et al. (2016)
8.41
Mitochondrial respirasome
(I
1 III
2 IV
1 supercomplex)
A8-35
●
Comparative absorbance and
RRS spectra of mitochondrial
complexes in isolation and
integrated into a respiratory
supercomplex
Shinzawa-Itoh et al. (2016)
8.42
Yeast cytochrome
c
oxidase (Complex IV)
SMA
●
The binding or unbinding of
ligands (O
2 , CO) to Complex
IV was studied by TRAS
Smirnova et al. (2016)
8.43
No protein
SMA
●
Time-resolved FRET and
SANS were used to determine
the kinetics and mechanisms of
phospholipid transfer among
SMALPs
Cuevas Arenas et al. (2017)
(continued)
8.2 Optical Spectroscopy Studies of Amphipols and Membrane Protein/Amphipol Complexes
389