Table 8.1
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.44
Outer membrane
phospholipaseA (OmpLA)
DIBMA
●
Comparative UV absorbance
spectra of DIBMA and SMA;
comparative Raman spectra of
lipids in vesicles, DIBMALPs,
and SMALPs
Oluwole et al. (2017)
Abbreviations: ABCG2
ATP-binding cassette subfamily G member 2, BLT1 and BLT2,
respectively, Type 1 and 2 leukotriene GPCRs, BR
bacteriorhodopsin, CAC
critical
aggregation concentration, CB1
Type 1 cannabinoid GPCR, CD
circular dichroism, CFTR
cystic
fibrosis transmembrane conductance regulator, CHO cells
Chinese hamster
ovary cells, DIBMA
diisobutylene-maleic acid copolymer, DIBMALPs
IBMA/lipid particles, DLS
dynamic light scattering, DSC
differential scanning calorimetry, EII
mtl
mannitol
permease, EM
electron microscopy, FAPol
a
fluorescently labeled derivative of A8-35, FAPol
AF647 Alexa Fluor 647-labeled A8-35, FAPol
fluo fluorescein-labeled A8-35,
FAPol
NBD NBD-labeled A8-35, FAPol
rhod rhodamine-labeled A8-35, FCS
fluorescence correlation spectroscopy, FRET
Förster resonance energy transfer, GLUTA
insulinresponsive facilitative glucose transporter, GpA
glycophorin A, HPMA N-(2-hydroxypropyl)-methacrylamide, 5-HT
4(a) Type 4(a) serotonin GPCR, IgG
immunoglobulin G, LHC
light-harvesting complex, LHCSR
stress-related light-harvesting complex, LMA
lauryl methacrylate, LS
light scattering, MOMP
major outer membrane protein from Chlamydia
trachomatis, MRP1
and MRP4
multidrug-resistance proteins 1 and 4, nAChR
nicotinic acetylcholine receptor, NBD
7-nitrobenz-2-oxa-1,3-diazol-4-yl group, OmpLA
outer
membrane phospholipase A, PAA
poly(acrylic acid), PagP
lipid A palmitoyltransferase, P-gp
P-glycoprotein 1, RRS
resonance Raman spectroscopy, SANS
small-angle neutron
scattering, SEC
size exclusion chromatography, SERCA1a
sarcoplasmic Ca
2+
-ATPase, SERS
surface-enhanced Raman scattering, SLS
static light scattering, SMA
styrene-maleic
acid copolymer, SMALPs
SMA/lipid particles, SRCD
synchrotron radiation CD, TRAS
time-resolved absorbance spectroscopy, V2R
arginine-vasopressin Type 2 GPCR
390
8 Optical Spectroscopy of Membrane Protein/Amphipol Complexes
(continued)
Study Protein
Amphipol
CD/
SRCD Fluorescence
RRS/
SERS TRAS Comments
References
8.44
Outer membrane
phospholipaseA (OmpLA)
DIBMA
●
Comparative UV absorbance
spectra of DIBMA and SMA;
comparative Raman spectra of
lipids in vesicles, DIBMALPs,
and SMALPs
Oluwole et al. (2017)
Abbreviations: ABCG2
ATP-binding cassette subfamily G member 2, BLT1 and BLT2,
respectively, Type 1 and 2 leukotriene GPCRs, BR
bacteriorhodopsin, CAC
critical
aggregation concentration, CB1
Type 1 cannabinoid GPCR, CD
circular dichroism, CFTR
cystic
fibrosis transmembrane conductance regulator, CHO cells
Chinese hamster
ovary cells, DIBMA
diisobutylene-maleic acid copolymer, DIBMALPs
IBMA/lipid particles, DLS
dynamic light scattering, DSC
differential scanning calorimetry, EII
mtl
mannitol
permease, EM
electron microscopy, FAPol
a
fluorescently labeled derivative of A8-35, FAPol
AF647 Alexa Fluor 647-labeled A8-35, FAPol
fluo fluorescein-labeled A8-35,
FAPol
NBD NBD-labeled A8-35, FAPol
rhod rhodamine-labeled A8-35, FCS
fluorescence correlation spectroscopy, FRET
Förster resonance energy transfer, GLUTA
insulinresponsive facilitative glucose transporter, GpA
glycophorin A, HPMA N-(2-hydroxypropyl)-methacrylamide, 5-HT
4(a) Type 4(a) serotonin GPCR, IgG
immunoglobulin G, LHC
light-harvesting complex, LHCSR
stress-related light-harvesting complex, LMA
lauryl methacrylate, LS
light scattering, MOMP
major outer membrane protein from Chlamydia
trachomatis, MRP1
and MRP4
multidrug-resistance proteins 1 and 4, nAChR
nicotinic acetylcholine receptor, NBD
7-nitrobenz-2-oxa-1,3-diazol-4-yl group, OmpLA
outer
membrane phospholipase A, PAA
poly(acrylic acid), PagP
lipid A palmitoyltransferase, P-gp
P-glycoprotein 1, RRS
resonance Raman spectroscopy, SANS
small-angle neutron
scattering, SEC
size exclusion chromatography, SERCA1a
sarcoplasmic Ca
2+
-ATPase, SERS
surface-enhanced Raman scattering, SLS
static light scattering, SMA
styrene-maleic
acid copolymer, SMALPs
SMA/lipid particles, SRCD
synchrotron radiation CD, TRAS
time-resolved absorbance spectroscopy, V2R
arginine-vasopressin Type 2 GPCR
390
8 Optical Spectroscopy of Membrane Protein/Amphipol Complexes