Study Amphipol(s) Protein(s) a
Characterization of membrane protein/amphipol
particle solution properties by:
Comments
References
Compositional
analysis
SANS SG-AUC SV-AUC Eq-AUC
SEC DLS CD EM NMR Other methods
5.32 A8-35
Cytochrome
bc1 complex
●
●
●
●
SAXS
A study of the interactions in solution and
crystallization of bc1/A8-35 complexes and of the
effect of adding salt or polyethylene glycol and of
APol depletion.
Charvolin et al.
(2014) see also
Popot et al. (2003)
5.33 A8-35
OmpX
●
A comparison of the structure, particle size, and
accessibility from the solvent of OmpX trapped in
A8-35 or solubilized in diC6PC.
Etzkorn et al.
(2014)
5.34 SMA
PgP
●
●
●
A low-resolution (~3.5 nm) cryo-EM study of PgP
extracted from insect cell membranes with SMA.
Gulati et al.
(2014)
5.35 A8-35
OmpX
MD
Molecular dynamics simulations of an
OmpX/A8-35 complex.
Perlmutter et al.
(2014)
5.36 A8-35
ExbB-ExbD
complex
●
●
●
●
●
SAXS
An extensive study of ExbB-ExbD/A8-35
complexes.
Sverzhinsky
et al. (2014)
5.37 SMA
Photosynthetic
reaction center
●
●
Absorbance
spectroscopy
A study of the functionality and stability of
bacterial photosynthetic reaction centers extracted
with SMA and of the size and composition of the
resulting particles.
Swainsbury et al.
(2014)
5.38 PMAL-C8
TRPA1
●
A single-particle cryo-EM studies of TRPA1
showing the protein-bound APol belt at 6-Å
resolution.
Paulsen et al.
(2015)
5.39 SMA
AcrB
●
●
●
The AcrB trimer was extracted from E. coli
membranes with SMA. SV-AUC and EM imaging
after negative staining show that the particles can
form dimers associated via the SMA/lipid belts in
an ionic strength-dependent manner. Lipid analysis
shows that the particles contain only about half the
amount of lipids needed to fully protect the
transmembrane region from contact with SMA.
Postis et al.
(2015)
5.40 A8-35
PagP, OmpT,
Mhp1, GalP
ESI-IMS-MS
A comparative study of the conformational
stability of MPs when transferred to the gas phase
either from DDM or from A8-35.
Calabrese et al.
(2015) see
also Watkinson
et al. (2015)
(continued)
5.3 Composition, Organization, Dynamics, and Solution Properties of Membrane. . .
271
Characterization of membrane protein/amphipol
particle solution properties by:
Comments
References
Compositional
analysis
SANS SG-AUC SV-AUC Eq-AUC
SEC DLS CD EM NMR Other methods
5.32 A8-35
Cytochrome
bc1 complex
●
●
●
●
SAXS
A study of the interactions in solution and
crystallization of bc1/A8-35 complexes and of the
effect of adding salt or polyethylene glycol and of
APol depletion.
Charvolin et al.
(2014) see also
Popot et al. (2003)
5.33 A8-35
OmpX
●
A comparison of the structure, particle size, and
accessibility from the solvent of OmpX trapped in
A8-35 or solubilized in diC6PC.
Etzkorn et al.
(2014)
5.34 SMA
PgP
●
●
●
A low-resolution (~3.5 nm) cryo-EM study of PgP
extracted from insect cell membranes with SMA.
Gulati et al.
(2014)
5.35 A8-35
OmpX
MD
Molecular dynamics simulations of an
OmpX/A8-35 complex.
Perlmutter et al.
(2014)
5.36 A8-35
ExbB-ExbD
complex
●
●
●
●
●
SAXS
An extensive study of ExbB-ExbD/A8-35
complexes.
Sverzhinsky
et al. (2014)
5.37 SMA
Photosynthetic
reaction center
●
●
Absorbance
spectroscopy
A study of the functionality and stability of
bacterial photosynthetic reaction centers extracted
with SMA and of the size and composition of the
resulting particles.
Swainsbury et al.
(2014)
5.38 PMAL-C8
TRPA1
●
A single-particle cryo-EM studies of TRPA1
showing the protein-bound APol belt at 6-Å
resolution.
Paulsen et al.
(2015)
5.39 SMA
AcrB
●
●
●
The AcrB trimer was extracted from E. coli
membranes with SMA. SV-AUC and EM imaging
after negative staining show that the particles can
form dimers associated via the SMA/lipid belts in
an ionic strength-dependent manner. Lipid analysis
shows that the particles contain only about half the
amount of lipids needed to fully protect the
transmembrane region from contact with SMA.
Postis et al.
(2015)
5.40 A8-35
PagP, OmpT,
Mhp1, GalP
ESI-IMS-MS
A comparative study of the conformational
stability of MPs when transferred to the gas phase
either from DDM or from A8-35.
Calabrese et al.
(2015) see
also Watkinson
et al. (2015)
(continued)
5.3 Composition, Organization, Dynamics, and Solution Properties of Membrane. . .
271