Table 5.4
(continued)
5.8
A8-35
tOmpA
●
The complexes formed between A8-35 and the
transmembrane β-barrel domain of OmpA have
been studied by solution NMR. The rotational
correlation time τc of the particles has been
determined and the points of contact between the
two partners have been mapped.
Zoonens et al.
(2005)
5.9
A8-35
OmpA, FomA,
BR
●
●
Three membrane proteins have been folded in
A8-35 and their final state studied by SDS-PAGE,
CD, and functional measurements.
Pocanschi et al.
(2006)
5.10
A8-35
BR, b
6 f
●
ITC
BR and cytochrome b6 f were trapped in either
A8-35 or various APols and the complexes studied
by centrifugation on sucrose gradients and visible
spectroscopy. The effects of pH, high salt, and Ca 2+
ions have been examined, as well as the enthalpy
change associated with substituting APol for
detergent at the surface of BR.
Diab et al. (2007)
PC-APols
5.11
A8-35
tOmpA
●
FRET
The stability of tOmpA/A8-35 complexes upon
removal of the free APol has been studied, as well
as the kinetics of exchange of a protein-bound
fluorescent APol for an unlabeled APol or for
detergent.
Zoonens et al.
(2007)
5.12 A8-35
BR
●
●
●
●
●
●
An extensive examination of the composition, size,
and internal organization of BR/lipid/A8-35
particles, of the functional state of BR, and of the
effect of removing excess APol from the solutions.
Gohon et al.
(2008)
Study Amphipol(s) Protein(s) a
Characterization of membrane protein/amphipol
particle solution properties by:
Comments
References
Compositional
analysis
SANS SG-AUC SV-AUC Eq-AUC SEC DLS CD EM NMR Other methods
Cross-linking +
SDS-PAGE
Diacylglycerol kinase was trapped either with
OAPA-20 (≈ A8-75) or with PMAL-B (= PMALC12) and its state of oligomerization and
aggregation studied by cross-linking with
glutaraldehyde followed by SDS-PAGE. In addition,
the transfer of DAGK from APols to lipid vesicles
was demonstrated.
Nagy et al. (2001)
5.6
OAPA-20
DAGK
PMAL-B
(= PMALC12)
5.7
A8-35
nAChR
●
The nicotinic acetylcholine receptor was trapped in
A8-35 and the migration of its monomeric and
dimeric forms in sucrose gradients compared to that
in the presence of CHAPS. Allosteric transitions
induced by the binding of an analog of acetylcholine
were studied by fluorescence spectroscopy.
Martinez et al.
(2002)
268
5 Formation and Properties of Membrane Protein/Amphipol Complexes
(continued)
5.8
A8-35
tOmpA
●
The complexes formed between A8-35 and the
transmembrane β-barrel domain of OmpA have
been studied by solution NMR. The rotational
correlation time τc of the particles has been
determined and the points of contact between the
two partners have been mapped.
Zoonens et al.
(2005)
5.9
A8-35
OmpA, FomA,
BR
●
●
Three membrane proteins have been folded in
A8-35 and their final state studied by SDS-PAGE,
CD, and functional measurements.
Pocanschi et al.
(2006)
5.10
A8-35
BR, b
6 f
●
ITC
BR and cytochrome b6 f were trapped in either
A8-35 or various APols and the complexes studied
by centrifugation on sucrose gradients and visible
spectroscopy. The effects of pH, high salt, and Ca 2+
ions have been examined, as well as the enthalpy
change associated with substituting APol for
detergent at the surface of BR.
Diab et al. (2007)
PC-APols
5.11
A8-35
tOmpA
●
FRET
The stability of tOmpA/A8-35 complexes upon
removal of the free APol has been studied, as well
as the kinetics of exchange of a protein-bound
fluorescent APol for an unlabeled APol or for
detergent.
Zoonens et al.
(2007)
5.12 A8-35
BR
●
●
●
●
●
●
An extensive examination of the composition, size,
and internal organization of BR/lipid/A8-35
particles, of the functional state of BR, and of the
effect of removing excess APol from the solutions.
Gohon et al.
(2008)
Study Amphipol(s) Protein(s) a
Characterization of membrane protein/amphipol
particle solution properties by:
Comments
References
Compositional
analysis
SANS SG-AUC SV-AUC Eq-AUC SEC DLS CD EM NMR Other methods
Cross-linking +
SDS-PAGE
Diacylglycerol kinase was trapped either with
OAPA-20 (≈ A8-75) or with PMAL-B (= PMALC12) and its state of oligomerization and
aggregation studied by cross-linking with
glutaraldehyde followed by SDS-PAGE. In addition,
the transfer of DAGK from APols to lipid vesicles
was demonstrated.
Nagy et al. (2001)
5.6
OAPA-20
DAGK
PMAL-B
(= PMALC12)
5.7
A8-35
nAChR
●
The nicotinic acetylcholine receptor was trapped in
A8-35 and the migration of its monomeric and
dimeric forms in sucrose gradients compared to that
in the presence of CHAPS. Allosteric transitions
induced by the binding of an analog of acetylcholine
were studied by fluorescence spectroscopy.
Martinez et al.
(2002)
268
5 Formation and Properties of Membrane Protein/Amphipol Complexes