Table 5.3 (continued)
Initial environment Amphipol
a
Method of transfer
Comments
References
Dodecylmaltoside
(DDM)
(CMC % 0.17 mM)
A8-35,
PMALC12,
SAPols
Dilution
Seldom applicable
because of the low CMC
of DDM
Champeil et al. (2000)
and Picard et al. (2006)
A8-35,
NAPols
Bio-Beads
More generally
applicable than dilution
Charvolin et al. (2009),
Bazzacco et al. (2012),
Cao et al. (2013), and
Liao et al. (2013)
A8-35
SEC
The protein in DDM
solution was
supplemented with
A8-35, concentrated by
ultrafiltration, and
separated from DDM by
SEC in the absence of
surfactant
Kevany et al. (2013) and
Tsybovsky et al. (2013)
Hecameg
(CMC % 19.5 mM)
A8-35,
NAPols
Dilution
See comments given for
C 8 -POE/C 8 E 4
Tribet et al. (1996) and
Prata et al. (2001)
Octylglucoside (OG)
(CMC %
20–25 mM)
A8-35,
NAPols
Dilution
See comments given for
C 8 -POE/C 8 E 4
Tribet et al. (1996),
Prata et al. (2001),
Sharma et al. (2008),
and Bazzacco et al.
(2009, 2012)
Octylthioglucoside
(OTG)
(CMC % 9 mM)
Bio-Beads
Gohon et al. (2008),
Charvolin et al. (2009),
and Sharma et al. (2012)
Sarkosyl
(CMC % 13.7 mM)
APG and a
variant
thereof
Dialysis
According to SEC
analysis, transfer from
sarkosyl to APG is
accompanied by a large
size increase
Han et al. (2014, 2017)
Biological
membranes or
proteoliposomes
SMA
Direct extraction
Essentially specific to
SMA. Cases of direct
extraction by A8-35 have
been observed, but not
studied in detail (see text,
§ 5.2.2.2, and Box 5.1)
Knowles et al. (2009),
Jamshad et al. (2011),
Orwick-Rydmark et al.
(2012), Gulati et al.
(2014), Postis et al.
(2015), Dörr et al.
(2016), Lee and Pollock
(2016), and Logez et al.
(2016)
(A8-35)
Methods starting from an unfolded protein or a synthetic peptide
SDS
A8-35,
Precipitation of
potassium dodecyl
sulfate (PDS)
The method of choice for
refolding α-helical MPs
from SDS. A membrane
protein unfolded in
organic solvent can be
first transferred to SDS,
where it acquires some αhelicity, and then folded
by transfer to APols. See
Chap. 6
Pocanschi et al. (2006b),
Dahmane et al. (2009,
2011, 2013), Catoire
et al. (2010a), Bazzacco
et al. (2012), Elter et al.
(2014), and Popot
(2014)
SAPols,
NAPols
A8-35
PDS precipitation,
dialysis, dilution,
or Bio-Beads
These two articles
present interesting
comparisons of the yields
and degrees of
monodispersity achieved
using various procedures
for folding BR by
transfer from SDS to
A8-35. See Chap. 6
Dahmane et al. (2013)
and Elter et al. (2014)
(continued)
5.2 Forming Membrane Protein/Amphipol Complexes
251
Initial environment Amphipol
a
Method of transfer
Comments
References
Dodecylmaltoside
(DDM)
(CMC % 0.17 mM)
A8-35,
PMALC12,
SAPols
Dilution
Seldom applicable
because of the low CMC
of DDM
Champeil et al. (2000)
and Picard et al. (2006)
A8-35,
NAPols
Bio-Beads
More generally
applicable than dilution
Charvolin et al. (2009),
Bazzacco et al. (2012),
Cao et al. (2013), and
Liao et al. (2013)
A8-35
SEC
The protein in DDM
solution was
supplemented with
A8-35, concentrated by
ultrafiltration, and
separated from DDM by
SEC in the absence of
surfactant
Kevany et al. (2013) and
Tsybovsky et al. (2013)
Hecameg
(CMC % 19.5 mM)
A8-35,
NAPols
Dilution
See comments given for
C 8 -POE/C 8 E 4
Tribet et al. (1996) and
Prata et al. (2001)
Octylglucoside (OG)
(CMC %
20–25 mM)
A8-35,
NAPols
Dilution
See comments given for
C 8 -POE/C 8 E 4
Tribet et al. (1996),
Prata et al. (2001),
Sharma et al. (2008),
and Bazzacco et al.
(2009, 2012)
Octylthioglucoside
(OTG)
(CMC % 9 mM)
Bio-Beads
Gohon et al. (2008),
Charvolin et al. (2009),
and Sharma et al. (2012)
Sarkosyl
(CMC % 13.7 mM)
APG and a
variant
thereof
Dialysis
According to SEC
analysis, transfer from
sarkosyl to APG is
accompanied by a large
size increase
Han et al. (2014, 2017)
Biological
membranes or
proteoliposomes
SMA
Direct extraction
Essentially specific to
SMA. Cases of direct
extraction by A8-35 have
been observed, but not
studied in detail (see text,
§ 5.2.2.2, and Box 5.1)
Knowles et al. (2009),
Jamshad et al. (2011),
Orwick-Rydmark et al.
(2012), Gulati et al.
(2014), Postis et al.
(2015), Dörr et al.
(2016), Lee and Pollock
(2016), and Logez et al.
(2016)
(A8-35)
Methods starting from an unfolded protein or a synthetic peptide
SDS
A8-35,
Precipitation of
potassium dodecyl
sulfate (PDS)
The method of choice for
refolding α-helical MPs
from SDS. A membrane
protein unfolded in
organic solvent can be
first transferred to SDS,
where it acquires some αhelicity, and then folded
by transfer to APols. See
Chap. 6
Pocanschi et al. (2006b),
Dahmane et al. (2009,
2011, 2013), Catoire
et al. (2010a), Bazzacco
et al. (2012), Elter et al.
(2014), and Popot
(2014)
SAPols,
NAPols
A8-35
PDS precipitation,
dialysis, dilution,
or Bio-Beads
These two articles
present interesting
comparisons of the yields
and degrees of
monodispersity achieved
using various procedures
for folding BR by
transfer from SDS to
A8-35. See Chap. 6
Dahmane et al. (2013)
and Elter et al. (2014)
(continued)
5.2 Forming Membrane Protein/Amphipol Complexes
251