Bacterial
translocon
(SecYEG)
E. coli, plasma
membrane
α
3
Voltage-gated
potassium channel
Kv1.3
Human, plasma
membrane
α
4
× 1
5
× 1
4
× 1
Serotonin 5-HT
3a
receptor
Human, plasma
membrane
α
Transient receptor
potential
melastatin 4
channel (TRPM4)
Human, plasma
membrane
α
~668
kDa
Native, DDM or
LMNG
Melatonin
receptor (MT1R)
Human, plasma
membrane
α
1
39 kDa
Native, Pichia
pastoris
membranes
Native,
DDM or C12E9
~75 kDa Native, E. coli
membrane
SMA
Ligand binding
Prabudiansyah et al. (2015)
~232
kDa
Native,
fos-choline-12
A8-35
BN-PAGE,
NS-EM
Spear et al. (2015)
~260
kDa
A8-35
NS-EM
Wu et al. (2015)
A8-35
BN-PAGE,
SEC, NS-EM
Constantine et al. (2016)
SMA
Ligand binding, G protein
activation and arrestin
recruitment
Logez et al. (2016)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
F1Fo
ATP synthase
dimer and subcomplexes
Polytomella sp.,
inner
mitochondrial
membrane
α
A8-35
Progressive dislocation of
the superdimer by high
concentrations of A8-35
examined by BN-PAGE
Vázquez-Acevedo et al. (2016)
STRA6 receptor
for retinol uptake
Zebrafish,
plasma
membrane
α
2 × 1 (+
calmodulin)
EF-handcontaining
transmembrane
protein 1 (LETM1;
residues 111-698)
Mouse,
mitochondrial
inner membrane
α
~404
kDa
Native,
fos-choline-12
Polycystic Kidney
Disease Channel-2
(PKD2)
Human, plasma
membrane
α
A8-35
Cryo-EM
Chen et al. (2016)
A8-35
NS-EM
Shao et al. (2016)
A8-35
Cryo-EM
Shen et al. (2016)
Holotranslocon
(SecYEG, SecDFYajC, YidC)
E. coli plasma
membrane
α
2 × 17
~1.6
MDa
Native, detergent
~180
kDa
Native, LMNG
~325
kDa
Native, DDM
7
~250
kDa
Native, DDM
A8-35
Cryo-EM, SANS
Botte et al. (2016)
Engineered
AcrAB-TolC
multidrug efflux
pump
E. coli plasma
and outer
membranes
α + β
(12;
partially
fused)
HasR heme
transporter and
HasR/HasA
complex
Serratia
marcescens
outer
membrane
β
1 and 2
~95 and
~115
kDa
Native,
Zwittergent 3-14
A8-35
Cryo-EM
Jeong et al. (2016)
A8-35
NS-EM
Wojtowicz et al. (2016)
4
× 1
6
× 1
~760
kDa
Native, DDM
246
5 Formation and Properties of Membrane Protein/Amphipol Complexes
translocon
(SecYEG)
E. coli, plasma
membrane
α
3
Voltage-gated
potassium channel
Kv1.3
Human, plasma
membrane
α
4
× 1
5
× 1
4
× 1
Serotonin 5-HT
3a
receptor
Human, plasma
membrane
α
Transient receptor
potential
melastatin 4
channel (TRPM4)
Human, plasma
membrane
α
~668
kDa
Native, DDM or
LMNG
Melatonin
receptor (MT1R)
Human, plasma
membrane
α
1
39 kDa
Native, Pichia
pastoris
membranes
Native,
DDM or C12E9
~75 kDa Native, E. coli
membrane
SMA
Ligand binding
Prabudiansyah et al. (2015)
~232
kDa
Native,
fos-choline-12
A8-35
BN-PAGE,
NS-EM
Spear et al. (2015)
~260
kDa
A8-35
NS-EM
Wu et al. (2015)
A8-35
BN-PAGE,
SEC, NS-EM
Constantine et al. (2016)
SMA
Ligand binding, G protein
activation and arrestin
recruitment
Logez et al. (2016)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
F1Fo
ATP synthase
dimer and subcomplexes
Polytomella sp.,
inner
mitochondrial
membrane
α
A8-35
Progressive dislocation of
the superdimer by high
concentrations of A8-35
examined by BN-PAGE
Vázquez-Acevedo et al. (2016)
STRA6 receptor
for retinol uptake
Zebrafish,
plasma
membrane
α
2 × 1 (+
calmodulin)
EF-handcontaining
transmembrane
protein 1 (LETM1;
residues 111-698)
Mouse,
mitochondrial
inner membrane
α
~404
kDa
Native,
fos-choline-12
Polycystic Kidney
Disease Channel-2
(PKD2)
Human, plasma
membrane
α
A8-35
Cryo-EM
Chen et al. (2016)
A8-35
NS-EM
Shao et al. (2016)
A8-35
Cryo-EM
Shen et al. (2016)
Holotranslocon
(SecYEG, SecDFYajC, YidC)
E. coli plasma
membrane
α
2 × 17
~1.6
MDa
Native, detergent
~180
kDa
Native, LMNG
~325
kDa
Native, DDM
7
~250
kDa
Native, DDM
A8-35
Cryo-EM, SANS
Botte et al. (2016)
Engineered
AcrAB-TolC
multidrug efflux
pump
E. coli plasma
and outer
membranes
α + β
(12;
partially
fused)
HasR heme
transporter and
HasR/HasA
complex
Serratia
marcescens
outer
membrane
β
1 and 2
~95 and
~115
kDa
Native,
Zwittergent 3-14
A8-35
Cryo-EM
Jeong et al. (2016)
A8-35
NS-EM
Wojtowicz et al. (2016)
4
× 1
6
× 1
~760
kDa
Native, DDM
246
5 Formation and Properties of Membrane Protein/Amphipol Complexes