Equilibrative
nucleoside
transporter-1
(hENT1)
Human, plasma
and
mitochondrial
membranes
α
Native, Sf9
membranes
SMA
Rehan et al. (2017)
Pneumolysin
Streptococcus
pneumoniae,
secreted toxin
β
A8-35
AcrABZ-TolC
multidrug efflux
pump
E. coli plasma
and outer
membranes
α + β
A8-35
Transient receptor
potential channel
polycystin-2 (PC2)
Human,
α
1
~50 kDa
42
~2.2
MDa
Native, Cymal-6
12
~770
kDa
Native, DDM
4
~440
kDa
Native,
LMNG/CHS
A8-35
Cryo-EM
van Pee et al. (2017)
Cryo-EM
Wang et al. (2017)
Cryo-EM
Wilkes et al. (2017)
Ligand binding,
SEC
Cation diffusion
facilitator CzcD
Cupriavidus
metallidurans
CH34
α
2
× 1
4
× 1
~68 kDa Native, E. coli
plasma membrane
SMA
DLS, solid-state NMR
Bersch et al. (2017)
Lysophosphatidic
acid receptor 2
(LPA2)
Human, plasma
membrane
α
1
Fusion
protein
Native, sarkosyl
Variant of
APG
Ligand-dependent G protein
binding
Han et al. (2017)
Cyclic-nucleotidegated channel
(TAX-4)
Caenorhabditis
elegans, plasma
membrane
α
~335
kDa
Native, LMNG +
lipids
A8-35
Cryo-EM
Li et al. (2017)
Outer membrane
phospholipase A
(OmpLA)
E. coli, outer
membrane
β
1
~31 kDa
Native,
proteoliposomes
DIBMA
Enzymatic activity, CD,
NS-EM, DLS, SEC
Oluwole et al. (2017)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Abbreviations:
α-DDM n-dodecyl-α-D-maltoside, APG
amphipathic poly-γ-glutamic acid, APol
amphipol, BLM
black lipid membrane, BN-PAGE
blue-native polyacrylamide gel
electrophoresis, C
8 E
4 tetraethylene glycol monooctyl ether, C
8 -POE
octylpolyoxyethylene, C
10 -PEG
decylpoly(ethyleneglycol) 300, C
12 E
10 polyoxyethylene(10)dodecyl ether, CD
circular dichroism, CHAPS
3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate, CHAPSO
3-([3-cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate,
CHS
cholesterol hemisuccinate, cryo-EM
electron cryo-microscopy, DDM n-dodecyl-β-D-maltoside, DIBMA
diisobutylene/maleic acid copolymer, DLS
dynamic light scattering,
DM n-decyl-β-D-maltoside, diC
6 PC
1,2-dihexanoyl-sn-glycero-3-phosphocholine, DMPC
1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMSO
dimethyl sulfoxide, Empigen
a
trademark for n-dodecyl-N,N-dimethylglycine, DOC
sodium deoxycholate, EPR DEER
double electron-electron resonance electron paramagnetic resonance, ESI-IMS-MS
electrospray
ionization mass spectrometry coupled with ion mobility spectrometry, fos-choline-12
dodecylphosphorylcholine, FRET
Förster resonance energy transfer, GPCR
G protein-coupled
receptor, H69AR
small-cell lung cancer cell line, Hecameg
6-O-(N-heptylcarbamoyl)-methyl-α-D-glycopyranoside, HEK
human embryonic kidney cell line, LDAO N,N-dimethyldodecylamine-N-oxide, LMNG
lauryl maltose neopentyl glycol, 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside, MD
molecular dynamics simulations; MNG-3
¼ LMNG, NAPols
non-ionic APols (glucosylated unless otherwise indicated), NMR
nuclear magnetic resonance, NS-EM
electron microscopy in negative stain, OG n-octyl-β-D-glucopyranoside, OTG noctyl-β-D-thioglucopyranoside, PC-APols
phosphorylcholine-based APols, POPC
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPG
1-palmitoyl-2-oleoyl-sn-glycero-3phospho-(1
0
-rac-glycerol), SANS
small-angle neutron scattering, SAPols
sulfonated APols, SAXS
small-angle X-ray scattering, SDS
sodium dodecyl sulfate, SDS-PAGE
polyacrylamide gel electrophoresis in the presence of SDS, SEC
size exclusion chromatography, SG-AUC
sucrose gradient sedimentation velocity analytical ultracentrifugation, SMA
styrenemaleic acid copolymer, ssNMR
solid-state NMR, STEM
scanning transmission electron microscopy, SV-AUC
sedimentation velocity analytical ultracentrifugation, TFE
trifluoroethanol
5.2 Forming Membrane Protein/Amphipol Complexes
247
nucleoside
transporter-1
(hENT1)
Human, plasma
and
mitochondrial
membranes
α
Native, Sf9
membranes
SMA
Rehan et al. (2017)
Pneumolysin
Streptococcus
pneumoniae,
secreted toxin
β
A8-35
AcrABZ-TolC
multidrug efflux
pump
E. coli plasma
and outer
membranes
α + β
A8-35
Transient receptor
potential channel
polycystin-2 (PC2)
Human,
α
1
~50 kDa
42
~2.2
MDa
Native, Cymal-6
12
~770
kDa
Native, DDM
4
~440
kDa
Native,
LMNG/CHS
A8-35
Cryo-EM
van Pee et al. (2017)
Cryo-EM
Wang et al. (2017)
Cryo-EM
Wilkes et al. (2017)
Ligand binding,
SEC
Cation diffusion
facilitator CzcD
Cupriavidus
metallidurans
CH34
α
2
× 1
4
× 1
~68 kDa Native, E. coli
plasma membrane
SMA
DLS, solid-state NMR
Bersch et al. (2017)
Lysophosphatidic
acid receptor 2
(LPA2)
Human, plasma
membrane
α
1
Fusion
protein
Native, sarkosyl
Variant of
APG
Ligand-dependent G protein
binding
Han et al. (2017)
Cyclic-nucleotidegated channel
(TAX-4)
Caenorhabditis
elegans, plasma
membrane
α
~335
kDa
Native, LMNG +
lipids
A8-35
Cryo-EM
Li et al. (2017)
Outer membrane
phospholipase A
(OmpLA)
E. coli, outer
membrane
β
1
~31 kDa
Native,
proteoliposomes
DIBMA
Enzymatic activity, CD,
NS-EM, DLS, SEC
Oluwole et al. (2017)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Abbreviations:
α-DDM n-dodecyl-α-D-maltoside, APG
amphipathic poly-γ-glutamic acid, APol
amphipol, BLM
black lipid membrane, BN-PAGE
blue-native polyacrylamide gel
electrophoresis, C
8 E
4 tetraethylene glycol monooctyl ether, C
8 -POE
octylpolyoxyethylene, C
10 -PEG
decylpoly(ethyleneglycol) 300, C
12 E
10 polyoxyethylene(10)dodecyl ether, CD
circular dichroism, CHAPS
3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate, CHAPSO
3-([3-cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate,
CHS
cholesterol hemisuccinate, cryo-EM
electron cryo-microscopy, DDM n-dodecyl-β-D-maltoside, DIBMA
diisobutylene/maleic acid copolymer, DLS
dynamic light scattering,
DM n-decyl-β-D-maltoside, diC
6 PC
1,2-dihexanoyl-sn-glycero-3-phosphocholine, DMPC
1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMSO
dimethyl sulfoxide, Empigen
a
trademark for n-dodecyl-N,N-dimethylglycine, DOC
sodium deoxycholate, EPR DEER
double electron-electron resonance electron paramagnetic resonance, ESI-IMS-MS
electrospray
ionization mass spectrometry coupled with ion mobility spectrometry, fos-choline-12
dodecylphosphorylcholine, FRET
Förster resonance energy transfer, GPCR
G protein-coupled
receptor, H69AR
small-cell lung cancer cell line, Hecameg
6-O-(N-heptylcarbamoyl)-methyl-α-D-glycopyranoside, HEK
human embryonic kidney cell line, LDAO N,N-dimethyldodecylamine-N-oxide, LMNG
lauryl maltose neopentyl glycol, 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside, MD
molecular dynamics simulations; MNG-3
¼ LMNG, NAPols
non-ionic APols (glucosylated unless otherwise indicated), NMR
nuclear magnetic resonance, NS-EM
electron microscopy in negative stain, OG n-octyl-β-D-glucopyranoside, OTG noctyl-β-D-thioglucopyranoside, PC-APols
phosphorylcholine-based APols, POPC
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPG
1-palmitoyl-2-oleoyl-sn-glycero-3phospho-(1
0
-rac-glycerol), SANS
small-angle neutron scattering, SAPols
sulfonated APols, SAXS
small-angle X-ray scattering, SDS
sodium dodecyl sulfate, SDS-PAGE
polyacrylamide gel electrophoresis in the presence of SDS, SEC
size exclusion chromatography, SG-AUC
sucrose gradient sedimentation velocity analytical ultracentrifugation, SMA
styrenemaleic acid copolymer, ssNMR
solid-state NMR, STEM
scanning transmission electron microscopy, SV-AUC
sedimentation velocity analytical ultracentrifugation, TFE
trifluoroethanol
5.2 Forming Membrane Protein/Amphipol Complexes
247