Endothelin-1
receptor
Type A (ET
A )
Human, plasma
membrane
α
1
(fusion
protein)
Native in sarkosyl
APG
Ligand binding
Han et al. (2014)
γ-Secretase
complex
Human,
endoplasmic
reticulum
α
4
200–240
kDa
Native, digitonin
A8-35
Cryo-EM
Lu et al. (2014) and Bai et al. (2015)
FtsZ-PBP2-PBP2a
divisome
complexes
Staphylococcus
aureus, plasma
membrane
α
Unknown
Unknown Native, S. aureus
membranes
SMA
DLS, EM, flow cytometry
Paulin et al. (2014)
Ryanodine
receptor 1 (RyR1)
O. cuniculus,
sarcoplasmic
reticulum
α
4
2.3 MDa Native, CHAPS
A8-35
Cryo-EM
Baker et al. (2015) and Wei et al. (2016)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
NCS1 benzylhydantoin
transporter (Mhp1)
Microbacterium
liquefaciens,
plasma
membrane
α
Galactose
permease (GalP)
Streptococcus
thermophilus,
plasma
membrane
α
Mechanosensitive
Piezo1 channel
M. musculus,
plasma
membrane
α
Adenosine A2A
receptor (A2AR)
Human, plasma
membrane
α
Insulin-responsive
facilitative glucose
transporter
(GLUT4)
R. norvegicus,
plasma
membrane
α
E. coli tyrosine
kinase (ETK)
E. coli, plasma
membrane
α
Multidrug
transporter AcrB
E. coli, plasma
membrane
α
Tetraspanins
Human, plasma
membrane
α
variable
Hepatitis C virus
nonstructural
protein 5A
(NS5A)
Hepatitis C
virus, human
endoplasmic
reticulum
α
1
1
3
1
1
1
3
1
A8-35
Ligand binding,
ESI-IMS-MS
Calabrese et al. (2015)
A8-35
A8-35
Cryo-EM
Ge et al. (2015)
Native, Pichia
pastoris or human
HEK293T cell
membranes
SMA
Ligand binding, CD, SEC,
AUC
Jamshad et al. (2015)
A8-35
SEC, ligand
binding
Kraft et al. (2015)
Native, E. coli
membranes
SMA
NS-EM, ssNMR, autophosphorylation
Li et al. (2015)
Native, E. coli
membrane
SMA
Ligand binding, NS-EM
Postis et al. (2015)
variable Native, S. cerevisiae
membranes
Refolded,
fos-choline-12
54.6 kDa Native, DDM
51.7 kDa Native, DDM
~900
kDa
Native, C12E10
44.7 kDa
~55 kDa Native, LMNG
~80 kDa
~360
kDa
49 kDa
SMA
Skaar et al. (2015)
NAPol
NMR
Sólyom et al. (2015)
5.2 Forming Membrane Protein/Amphipol Complexes
245
receptor
Type A (ET
A )
Human, plasma
membrane
α
1
(fusion
protein)
Native in sarkosyl
APG
Ligand binding
Han et al. (2014)
γ-Secretase
complex
Human,
endoplasmic
reticulum
α
4
200–240
kDa
Native, digitonin
A8-35
Cryo-EM
Lu et al. (2014) and Bai et al. (2015)
FtsZ-PBP2-PBP2a
divisome
complexes
Staphylococcus
aureus, plasma
membrane
α
Unknown
Unknown Native, S. aureus
membranes
SMA
DLS, EM, flow cytometry
Paulin et al. (2014)
Ryanodine
receptor 1 (RyR1)
O. cuniculus,
sarcoplasmic
reticulum
α
4
2.3 MDa Native, CHAPS
A8-35
Cryo-EM
Baker et al. (2015) and Wei et al. (2016)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
NCS1 benzylhydantoin
transporter (Mhp1)
Microbacterium
liquefaciens,
plasma
membrane
α
Galactose
permease (GalP)
Streptococcus
thermophilus,
plasma
membrane
α
Mechanosensitive
Piezo1 channel
M. musculus,
plasma
membrane
α
Adenosine A2A
receptor (A2AR)
Human, plasma
membrane
α
Insulin-responsive
facilitative glucose
transporter
(GLUT4)
R. norvegicus,
plasma
membrane
α
E. coli tyrosine
kinase (ETK)
E. coli, plasma
membrane
α
Multidrug
transporter AcrB
E. coli, plasma
membrane
α
Tetraspanins
Human, plasma
membrane
α
variable
Hepatitis C virus
nonstructural
protein 5A
(NS5A)
Hepatitis C
virus, human
endoplasmic
reticulum
α
1
1
3
1
1
1
3
1
A8-35
Ligand binding,
ESI-IMS-MS
Calabrese et al. (2015)
A8-35
A8-35
Cryo-EM
Ge et al. (2015)
Native, Pichia
pastoris or human
HEK293T cell
membranes
SMA
Ligand binding, CD, SEC,
AUC
Jamshad et al. (2015)
A8-35
SEC, ligand
binding
Kraft et al. (2015)
Native, E. coli
membranes
SMA
NS-EM, ssNMR, autophosphorylation
Li et al. (2015)
Native, E. coli
membrane
SMA
Ligand binding, NS-EM
Postis et al. (2015)
variable Native, S. cerevisiae
membranes
Refolded,
fos-choline-12
54.6 kDa Native, DDM
51.7 kDa Native, DDM
~900
kDa
Native, C12E10
44.7 kDa
~55 kDa Native, LMNG
~80 kDa
~360
kDa
49 kDa
SMA
Skaar et al. (2015)
NAPol
NMR
Sólyom et al. (2015)
5.2 Forming Membrane Protein/Amphipol Complexes
245