Light-harvesting
complex II
(LHCII)
Arabidopsis
thaliana,
thylakoids
α
3
~75 kDa Native, α-DDM
A8-35
SEC, absorbance spectrum,
CD, fluorescence decay
Liguori et al. (2013) and Opačić et al. (2014)
NAPols
ExbB-ExbD
complex
E. coli, plasma
membrane
α
6
~140
kDa
Native, DDM
A8-35
BN-PAGE, SEC, SAXS,
SV-AUC, NS-EM
Sverzhinsky et al. (2014)
Mannitol
permease (EII
mtl
)
E. coli, plasma
membrane
α
2
136 kDa
Native, DOC or
C
10 -PEG
A8-35
Fluorescence emission
spectrum,
mannitol phosphorylation
activity
Opačić et al. (2014)
Potassium channel
KcsA
Streptomyces
lividans, plasma
membrane
α
4
41 kDa Native, E. coli
membranes
SMA
SEC, CD, SDS-PAGE,
fluorescence spectroscopy
Dörr et al. (2014)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
TRPV2 channel
Unspecified
α
Native, detergent
A8-35
Cryo-EM
Fan et al. (2014)
Rabbit, plasma
membrane
Native, DDM/lipid
mixture
Zubcevic et al. (2016)
CsgG secretion
channel
E. coli,
outer membrane
β
Native, C8E4/LDAO
/DMPC mixture
A8-35
NS-EM, binding of CsgE
Goyal et al. (2014)
Transient receptor
potential
melastatin 1
channel (TRPM1)
Human, plasma
membrane
α
Native, foscholine-14
A8-35
BN-PAGE, SEC, cryo-EM Agosto et al. (2014)
Melanocortin-2
receptor (MC2R)
Human, plasma
membrane
α
Denatured, SDS
A8-35
SEC, NMR (preliminary
data)
Elter et al. (2014)
Melanocortin-4
receptor (MC4R)
Human, plasma
membrane
α
Denatured, SDS
A8-35
P-glycoprotein
(ABCB1; PgP)
Human, plasma
membrane
α
Native, insect cell
membranes
SMA
Ligand binding, AUC, SEC,
cryo-EM
Gulati et al. (2014)
Multidrugresistance protein
1 (ABCC1; MRP1)
Human, plasma
membrane
α
Native, H69AR or
HEK cell
membranes
SMA
Ligand binding
Multidrugresistance protein 4
(ABCC4; MRP4)
Human, plasma
membrane
α
Native, insect or
HEK cell
membranes
SMA
ABCG2
Human, plasma
membrane
α
Native, insect cell
membranes
SMA
Ligand binding
Cystic fibrosis
transmembrane
regulator
(ABCC7; CFTR)
M. musculus,
plasma
membrane
α
Native, S. cerevisiae
microsomes
SMA
4
~385
kDa
9
~260
kDa
2
~360
kDa
1
~34 kDa
1
~37 kDa
1
170 kDa
1
190 kDa
1
150 kDa
2
~140
kDa
1
~170
kDa
244
5 Formation and Properties of Membrane Protein/Amphipol Complexes
complex II
(LHCII)
Arabidopsis
thaliana,
thylakoids
α
3
~75 kDa Native, α-DDM
A8-35
SEC, absorbance spectrum,
CD, fluorescence decay
Liguori et al. (2013) and Opačić et al. (2014)
NAPols
ExbB-ExbD
complex
E. coli, plasma
membrane
α
6
~140
kDa
Native, DDM
A8-35
BN-PAGE, SEC, SAXS,
SV-AUC, NS-EM
Sverzhinsky et al. (2014)
Mannitol
permease (EII
mtl
)
E. coli, plasma
membrane
α
2
136 kDa
Native, DOC or
C
10 -PEG
A8-35
Fluorescence emission
spectrum,
mannitol phosphorylation
activity
Opačić et al. (2014)
Potassium channel
KcsA
Streptomyces
lividans, plasma
membrane
α
4
41 kDa Native, E. coli
membranes
SMA
SEC, CD, SDS-PAGE,
fluorescence spectroscopy
Dörr et al. (2014)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
TRPV2 channel
Unspecified
α
Native, detergent
A8-35
Cryo-EM
Fan et al. (2014)
Rabbit, plasma
membrane
Native, DDM/lipid
mixture
Zubcevic et al. (2016)
CsgG secretion
channel
E. coli,
outer membrane
β
Native, C8E4/LDAO
/DMPC mixture
A8-35
NS-EM, binding of CsgE
Goyal et al. (2014)
Transient receptor
potential
melastatin 1
channel (TRPM1)
Human, plasma
membrane
α
Native, foscholine-14
A8-35
BN-PAGE, SEC, cryo-EM Agosto et al. (2014)
Melanocortin-2
receptor (MC2R)
Human, plasma
membrane
α
Denatured, SDS
A8-35
SEC, NMR (preliminary
data)
Elter et al. (2014)
Melanocortin-4
receptor (MC4R)
Human, plasma
membrane
α
Denatured, SDS
A8-35
P-glycoprotein
(ABCB1; PgP)
Human, plasma
membrane
α
Native, insect cell
membranes
SMA
Ligand binding, AUC, SEC,
cryo-EM
Gulati et al. (2014)
Multidrugresistance protein
1 (ABCC1; MRP1)
Human, plasma
membrane
α
Native, H69AR or
HEK cell
membranes
SMA
Ligand binding
Multidrugresistance protein 4
(ABCC4; MRP4)
Human, plasma
membrane
α
Native, insect or
HEK cell
membranes
SMA
ABCG2
Human, plasma
membrane
α
Native, insect cell
membranes
SMA
Ligand binding
Cystic fibrosis
transmembrane
regulator
(ABCC7; CFTR)
M. musculus,
plasma
membrane
α
Native, S. cerevisiae
microsomes
SMA
4
~385
kDa
9
~260
kDa
2
~360
kDa
1
~34 kDa
1
~37 kDa
1
170 kDa
1
190 kDa
1
150 kDa
2
~140
kDa
1
~170
kDa
244
5 Formation and Properties of Membrane Protein/Amphipol Complexes