Ferrichrome-iron
outer membrane
receptor (FhuA)
E. coli,
outer membrane
β
1
82 kDa Native, LDAO
PC-APol
Binding of viral protein
Basit et al. (2012)
T domain of
diphtheria toxin
Corynebacterium
diphtheriae,
secreted in
soluble form
(α)
1
~19 kDa Soluble form in
detergent-free buffer
NAPols
A study of conformational
transitions induced by low
pH and/or exposure to
NAPols
Kyrychenko et al. (2012)
Outer membrane
protein T (OmpT)
E. coli,
outer membrane
β
1
35 kDa
Denatured, urea
A8-35
SDS-PAGE, SEC, CD, ESIIMS-MS
Leney et al. (2012)
Refolded, DDM
or LDAO
A8-35
CD, protease activity, ESIIMS-MS, fast photochemical
oxidation of proteins
(FPOP)-LC-MS/MS
Calabrese et al. (2015) and Watkinson et al. (2015, 2017)
Refolded, LDAO
A8-75
Watkinson et al. (2015)
A34-35,
A34-75
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
NAPols
SAPols
Aquaporin
SoPIP2;1
Spinach, plasma
membrane
α
4
30 kDa Native, OG
A8-35
NS-EM
Vahedi-Faridi et al. (2013)
Capsaicin receptor
(TRPV1)
Rattus
norvegicus,
plasma
membrane
α
4
380 kDa Native, DDM
A8-35
Cryo-EM
Cao et al. (2013), Liao et al. (2013, 2014) and Gao et al. (2016)
Dhurrin metabolon
(CYP79A1,
CYP71E1,
cytochrome P450
oxidoreductase)
and subcomplexes
Sorghum
bicolor,
endoplasmic
reticulum
α
2–3
140200 kDa
Native,
Triton X-100
A8-35
SEC,
BN-PAGE,
enzymatic
activity, transfer
to liposomes
Laursen et al. (2013)
Native in Sorghum
bicolor microsomes
Laursen et al. (2016)
SMA
Cytochrome c
oxidase (COX)
S. cerevisiae,
inner
mitochondrial
membrane
α
11
~197
kDa
Native, S. cerevisiae
membranes
SMA
Enzymatic activity, lipid
analysis, NS-EM
Long et al. (2013) and Smirnova et al. (2016)
Photoreceptorspecific ABC
transporter
(ABCA4)
B. taurus,
retina discs
α
1
257 kDa Native, DDM
A8-35
SEC, ATPase activity, ATPinduced conformational
changes, NS-EM
Tsybovsky et al. (2013) and Zhang et al. (2015)
Peripherin-ROM1
complex
B. taurus,
retina discs
α
4
153 kDa Native, DDM
A8-35
NS-EM
Kevany et al. (2013)
KCNE1 (minK)
Human,
plasma
membrane
α
Uncertain
Uncertain Refolded,
POPC/POPG
vesicles
SMA
EPR DEER
measurements
Sahu et al. (2013)
5.2 Forming Membrane Protein/Amphipol Complexes
243
outer membrane
receptor (FhuA)
E. coli,
outer membrane
β
1
82 kDa Native, LDAO
PC-APol
Binding of viral protein
Basit et al. (2012)
T domain of
diphtheria toxin
Corynebacterium
diphtheriae,
secreted in
soluble form
(α)
1
~19 kDa Soluble form in
detergent-free buffer
NAPols
A study of conformational
transitions induced by low
pH and/or exposure to
NAPols
Kyrychenko et al. (2012)
Outer membrane
protein T (OmpT)
E. coli,
outer membrane
β
1
35 kDa
Denatured, urea
A8-35
SDS-PAGE, SEC, CD, ESIIMS-MS
Leney et al. (2012)
Refolded, DDM
or LDAO
A8-35
CD, protease activity, ESIIMS-MS, fast photochemical
oxidation of proteins
(FPOP)-LC-MS/MS
Calabrese et al. (2015) and Watkinson et al. (2015, 2017)
Refolded, LDAO
A8-75
Watkinson et al. (2015)
A34-35,
A34-75
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
NAPols
SAPols
Aquaporin
SoPIP2;1
Spinach, plasma
membrane
α
4
30 kDa Native, OG
A8-35
NS-EM
Vahedi-Faridi et al. (2013)
Capsaicin receptor
(TRPV1)
Rattus
norvegicus,
plasma
membrane
α
4
380 kDa Native, DDM
A8-35
Cryo-EM
Cao et al. (2013), Liao et al. (2013, 2014) and Gao et al. (2016)
Dhurrin metabolon
(CYP79A1,
CYP71E1,
cytochrome P450
oxidoreductase)
and subcomplexes
Sorghum
bicolor,
endoplasmic
reticulum
α
2–3
140200 kDa
Native,
Triton X-100
A8-35
SEC,
BN-PAGE,
enzymatic
activity, transfer
to liposomes
Laursen et al. (2013)
Native in Sorghum
bicolor microsomes
Laursen et al. (2016)
SMA
Cytochrome c
oxidase (COX)
S. cerevisiae,
inner
mitochondrial
membrane
α
11
~197
kDa
Native, S. cerevisiae
membranes
SMA
Enzymatic activity, lipid
analysis, NS-EM
Long et al. (2013) and Smirnova et al. (2016)
Photoreceptorspecific ABC
transporter
(ABCA4)
B. taurus,
retina discs
α
1
257 kDa Native, DDM
A8-35
SEC, ATPase activity, ATPinduced conformational
changes, NS-EM
Tsybovsky et al. (2013) and Zhang et al. (2015)
Peripherin-ROM1
complex
B. taurus,
retina discs
α
4
153 kDa Native, DDM
A8-35
NS-EM
Kevany et al. (2013)
KCNE1 (minK)
Human,
plasma
membrane
α
Uncertain
Uncertain Refolded,
POPC/POPG
vesicles
SMA
EPR DEER
measurements
Sahu et al. (2013)
5.2 Forming Membrane Protein/Amphipol Complexes
243