Lipid A
palmitoyltransferase
(PagP)
Native, DMPC
vesicle
Denatured, urea
Refolded, DDM
or LDAO
Refolded, LDAO
Respirasome,
supercomplex
I
1 III
2 IV
1
B. taurus,
mitochondrial
inner membrane
α
Complex I +
bc
1 dimer +
cytochrome
c oxidase
1.7 MDa
Native, digitonin
A8-35
Native, PCC-a-M
Transient receptor
potential ankyrin 1
ion channel
(TRPA1)
M. musculus,
plasma
membrane
α
4
512 kDa Native,
fos-choline 12
A8-35
E. coli,
outer membrane
β
1
22 kDa
SMA
CD, phospholipase activity Knowles et al. (2009)
A8-35
SDS-PAGE, SEC, CD,
ESI-IMS-MS
Leney et al. (2012)
A8-35
CD, enzymatic activity,
ESI-IMS-MS
Calabrese et al. (2015) and Watkinson et al. (2015)
A8-75
Watkinson et al. (2015)
A34-35,
A34-75
NAPols
SAPols
SG-AUC, BN-PAGE, in-gel
activity measurements,
cryo-EM
Althoff et al. (2011)
Enzymatic activity,
absorbance and resonance
Raman spectroscopies
Shinzawa-Itoh et al. (2016)
Enzymatic activity, cryo-EM Sousa et al. (2016)
CD, Ca
2+
flux after transfer
to lipid vesicles, NS-EM
Cvetkov et al. (2011) and Huynh et al. (2014)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Human, plasma
membrane
α
4
510 kDa Native, MNG-3
PMAL-C8
Cryo-EM
Paulsen et al. (2015)
Major outer
membrane protein
(MOMP)
Chlamydia
trachomatis,
outer membrane
β
3
~120
kDa
Native,
Zwittergent 3-14
A8-35
SDS-PAGE, CD
Tifrea et al. (2011, 2014, 2018a,b), and Cocco et al. (2013)
Corticotrophinreleasing factor
receptor 1
(CRFR1)
Human, plasma
membrane
α
1
47 kDa Nascent chain,
cell-free synthesis
NVoy
SEC, ligand
binding
Klammt et al. (2011)
Corticotrophinreleasing factor
receptor 2β
(CRFR2β)
M. musculus,
plasma
membrane
α
1
49 kDa Nascent chain,
cell-free synthesis
NVoy
SEC, ligand
binding
Ghrelin GHS-R1a
receptor
(GHS-R1a)
Human,
plasma
membrane
α
1
41 kDa
Denatured, SDS
A8-35
Ligand binding, G protein
activation and arrestin
recruitment
Damian et al. (2012)
g
Bazzacco et al. (2012)
NAPols
Refolded, asolectin
vesicles
Logez et al. (2016)
SMA
Vasopressin type 2
receptor (V2R)
Human,
plasma
membrane
α
1
42 kDa Native, detergent
NAPols
Ligand binding, G protein
activation and arrestin
recruitment, conformational
transitions
Rahmeh et al. (2012)
242
5 Formation and Properties of Membrane Protein/Amphipol Complexes
palmitoyltransferase
(PagP)
Native, DMPC
vesicle
Denatured, urea
Refolded, DDM
or LDAO
Refolded, LDAO
Respirasome,
supercomplex
I
1 III
2 IV
1
B. taurus,
mitochondrial
inner membrane
α
Complex I +
bc
1 dimer +
cytochrome
c oxidase
1.7 MDa
Native, digitonin
A8-35
Native, PCC-a-M
Transient receptor
potential ankyrin 1
ion channel
(TRPA1)
M. musculus,
plasma
membrane
α
4
512 kDa Native,
fos-choline 12
A8-35
E. coli,
outer membrane
β
1
22 kDa
SMA
CD, phospholipase activity Knowles et al. (2009)
A8-35
SDS-PAGE, SEC, CD,
ESI-IMS-MS
Leney et al. (2012)
A8-35
CD, enzymatic activity,
ESI-IMS-MS
Calabrese et al. (2015) and Watkinson et al. (2015)
A8-75
Watkinson et al. (2015)
A34-35,
A34-75
NAPols
SAPols
SG-AUC, BN-PAGE, in-gel
activity measurements,
cryo-EM
Althoff et al. (2011)
Enzymatic activity,
absorbance and resonance
Raman spectroscopies
Shinzawa-Itoh et al. (2016)
Enzymatic activity, cryo-EM Sousa et al. (2016)
CD, Ca
2+
flux after transfer
to lipid vesicles, NS-EM
Cvetkov et al. (2011) and Huynh et al. (2014)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Human, plasma
membrane
α
4
510 kDa Native, MNG-3
PMAL-C8
Cryo-EM
Paulsen et al. (2015)
Major outer
membrane protein
(MOMP)
Chlamydia
trachomatis,
outer membrane
β
3
~120
kDa
Native,
Zwittergent 3-14
A8-35
SDS-PAGE, CD
Tifrea et al. (2011, 2014, 2018a,b), and Cocco et al. (2013)
Corticotrophinreleasing factor
receptor 1
(CRFR1)
Human, plasma
membrane
α
1
47 kDa Nascent chain,
cell-free synthesis
NVoy
SEC, ligand
binding
Klammt et al. (2011)
Corticotrophinreleasing factor
receptor 2β
(CRFR2β)
M. musculus,
plasma
membrane
α
1
49 kDa Nascent chain,
cell-free synthesis
NVoy
SEC, ligand
binding
Ghrelin GHS-R1a
receptor
(GHS-R1a)
Human,
plasma
membrane
α
1
41 kDa
Denatured, SDS
A8-35
Ligand binding, G protein
activation and arrestin
recruitment
Damian et al. (2012)
g
Bazzacco et al. (2012)
NAPols
Refolded, asolectin
vesicles
Logez et al. (2016)
SMA
Vasopressin type 2
receptor (V2R)
Human,
plasma
membrane
α
1
42 kDa Native, detergent
NAPols
Ligand binding, G protein
activation and arrestin
recruitment, conformational
transitions
Rahmeh et al. (2012)
242
5 Formation and Properties of Membrane Protein/Amphipol Complexes