Outer membrane
protein A (OmpA)
or its
transmembrane
domain (tOmpA)
E. coli or
Klebsiella
pneumonia;
outer membrane
β
1
35 kDa
(OmpA)
19 kDa
(tOmpA)
Refolded, C
8 -POE
or C
8 E
4
A8-35
NAPols
Sharma et al. (2008) and Bazzacco et al. (2009)
SAPols
Dahmane et al. (2009)
Denatured, urea
A8-35
Pocanschi et al. (2006, 2013)
SAPols
SDS-PAGE
Dahmane et al. (2009)
Refolded, OG
A8-35
CD,
ESI-IMS-MS
Watkinson et al. (2015)
A8-75
A34-35,
A34-75
NAPols
SAPols
FomA
Fusobacterium
nucleatum,
outer membrane
β
1
40 kDa Denatured, urea
A8-35
SDS-PAGE, pore formation
in BLM
Pocanschi et al. (2006)
NADH
dehydrogenase
(Complex I)
Neurospora
crassa, inner
mitochondrial
membrane
α
~35 +
prosthetic
groups
1.1 MDa Native, Triton
X-100
A8-35
Cryo-EM
Flötenmeyer et al. (2007)
V-ATPase
transmembrane
peptides
Saccharomyces
cerevisiae,
plasma
membrane
α
1
3-4 kDa
A8-35
CD
Duarte et al. (2008)
f
Synthetic peptides,
TFE
SDS-PAGE,
NMR
Zoonens et al. (2005, 2007), Renault (2008), Sharma et al. (2008),
Charvolin et al. (2009), Dahmane et al. (2009), Bechara et al.
(2012), Della Pia et al. (2014), Le Bon et al. (2014), and Planchard
et al. (2014)
SDS-PAGE, NMR
SDS-PAGE, NMR
SDS-PAGE, tryptic
digestion, fluorescence, pore
formation in BLM
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Outer membrane
protein X (OmpX)
Leukotriene BLT1
receptor
Denatured, SDS
Leukotriene BLT2
receptor
Denatured, SDS
E. coli,
outer membrane
β
1
18.6 kDa
Refolded,
C
8 -POE or diC
6 PC
A8-35
SDS-PAGE, NMR, MD
Catoire et al. (2009, 2010b), Etzkorn et al. (2014), and Perlmutter
et al. (2014)
NAPols
SDS-PAGE, NMR
Bazzacco et al. (2012) and Sharma et al. (2012)
Human, plasma
membrane
α
1
38 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
Mus musculus,
plasma
membrane
α
1
41.5 kDa
A8-35
Ligand binding, ligandinduced conformational
changes
Dahmane et al. (2009), Catoire et al. (2010a, 2011), Banères et al.
(2011), Mary et al. (2014), and Casiraghi et al. (2016)
g
Serotonin 5-HT4a
receptor
Denatured, SDS
M. musculus,
plasma
membrane
α
1
44 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
Cannabinoid CB1
receptor
Denatured, SDS
M. musculus,
plasma
membrane
α
1
53 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
5.2 Forming Membrane Protein/Amphipol Complexes
241
protein A (OmpA)
or its
transmembrane
domain (tOmpA)
E. coli or
Klebsiella
pneumonia;
outer membrane
β
1
35 kDa
(OmpA)
19 kDa
(tOmpA)
Refolded, C
8 -POE
or C
8 E
4
A8-35
NAPols
Sharma et al. (2008) and Bazzacco et al. (2009)
SAPols
Dahmane et al. (2009)
Denatured, urea
A8-35
Pocanschi et al. (2006, 2013)
SAPols
SDS-PAGE
Dahmane et al. (2009)
Refolded, OG
A8-35
CD,
ESI-IMS-MS
Watkinson et al. (2015)
A8-75
A34-35,
A34-75
NAPols
SAPols
FomA
Fusobacterium
nucleatum,
outer membrane
β
1
40 kDa Denatured, urea
A8-35
SDS-PAGE, pore formation
in BLM
Pocanschi et al. (2006)
NADH
dehydrogenase
(Complex I)
Neurospora
crassa, inner
mitochondrial
membrane
α
~35 +
prosthetic
groups
1.1 MDa Native, Triton
X-100
A8-35
Cryo-EM
Flötenmeyer et al. (2007)
V-ATPase
transmembrane
peptides
Saccharomyces
cerevisiae,
plasma
membrane
α
1
3-4 kDa
A8-35
CD
Duarte et al. (2008)
f
Synthetic peptides,
TFE
SDS-PAGE,
NMR
Zoonens et al. (2005, 2007), Renault (2008), Sharma et al. (2008),
Charvolin et al. (2009), Dahmane et al. (2009), Bechara et al.
(2012), Della Pia et al. (2014), Le Bon et al. (2014), and Planchard
et al. (2014)
SDS-PAGE, NMR
SDS-PAGE, NMR
SDS-PAGE, tryptic
digestion, fluorescence, pore
formation in BLM
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Outer membrane
protein X (OmpX)
Leukotriene BLT1
receptor
Denatured, SDS
Leukotriene BLT2
receptor
Denatured, SDS
E. coli,
outer membrane
β
1
18.6 kDa
Refolded,
C
8 -POE or diC
6 PC
A8-35
SDS-PAGE, NMR, MD
Catoire et al. (2009, 2010b), Etzkorn et al. (2014), and Perlmutter
et al. (2014)
NAPols
SDS-PAGE, NMR
Bazzacco et al. (2012) and Sharma et al. (2012)
Human, plasma
membrane
α
1
38 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
Mus musculus,
plasma
membrane
α
1
41.5 kDa
A8-35
Ligand binding, ligandinduced conformational
changes
Dahmane et al. (2009), Catoire et al. (2010a, 2011), Banères et al.
(2011), Mary et al. (2014), and Casiraghi et al. (2016)
g
Serotonin 5-HT4a
receptor
Denatured, SDS
M. musculus,
plasma
membrane
α
1
44 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
Cannabinoid CB1
receptor
Denatured, SDS
M. musculus,
plasma
membrane
α
1
53 kDa
A8-35
Ligand binding
Dahmane et al. (2009), Banères et al. (2011), and Mary et al. (2014)
5.2 Forming Membrane Protein/Amphipol Complexes
241