Photosynthetic
reaction center
Rhodobacter
sphaeroides,
plasma
membrane
α
3
96 kDa
Native, Hecameg
A8-35
SG-AUC
Tribet et al. (1996)
A8-75
SG-AUC
Tribet et al. (1996, 1997)
A34-35,
A34-75
SG-AUC
Tribet et al. (1996)
Native, plasma
membrane
SMA
Spectrum, photochemistry
Swainsbury et al. (2014)
Glycophorin A
transmembrane
anchor (tGpA),
monomer or dimer
Human, plasma
membrane
α
1 or 2
~4 or
~8 kDa
Synthetic peptide,
urea
A5-75
CD
Gohon (1996) and Popot et al. (2003)
Synthetic peptides,
TFE
A8-35
CD, FRET, sequencespecific dimerization
Stangl et al. (2014)
F
1 F
o ATP
synthase
E. coli, plasma
membrane
α
21–24
~560
kDa
Native,
taurodeoxycholate
A8-75
NS-EM
Wilkens and Capaldi (1998a,b), Wilkens (2000), and Wilkens et al.
(2000)
Sarcoplasmic
calcium pump
(SERCA1a)
Oryctolagus
cuniculus,
sarcoplasmic
reticulum
α
1
110 kDa Native, DDM
A8-35
ATPase activity, SEC,
SV-AUC
Champeil et al. (2000) and Picard et al. (2006)
SAPol
ATPase activity, SEC
Picard et al. (2006)
PMAL-C12
e
,
PMALA-C12
ATPase activity,
SEC
Picard et al. (2006)
Diacylglycerol
kinase (DAGK)
E. coli,
plasma
membrane
α
3 × 1
40 kDa Native, Empigen
or DM
OAPA-20
e
,
PMAL-B-50,
PMAL-B-100
e
SDS-PAGE after chemical
cross-linking, ATPase
activity, MS
Nagy et al. (2001), Gorzelle et al. (2002), and Hopper et al. (2013)
Photosystem I
Synechocystis
PCC 6803,
thylakoids
α
3 × 12 +
> 120
cofactors
> 1 MDa Native, DDM
A8-35
Electrochemistry
Kievit and Brudvig (2001)
Nicotinic
acetylcholine
receptor (nAChR)
Torpedo
marmorata,
plasma
membrane
α
2 × 5
535 kDa Native, CHAPS
A8-35
Allosteric transitions, ligand
and toxin binding
Martinez et al. (2002) and Charvolin et al. (2009)
Rhodopsin
Bos taurus,
retina discs
α
1 + retinal
40 kDa
Native, CHAPS
A8-35
Transition to meta-II state
C. Tribet, C. Creuzenet & JLP, unpublished observations cited in
Popot et al. (2003)
Native, DDM
NS-EM (rhodopsin/GRK5
complex)
He et al. (2017)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Cytochrome bc
1
B. taurus, inner
mitochondrial
membrane
α
2 × 11
490 kDa Native, DDM
A8-35
NAPols
Bechara et al. (2012)
Catalysis of electron transfer,
SEC, SG-AUC, SAXS
Popot et al. (2003), Charvolin et al. (2009), Charvolin et al. (2014),
and Ferrandez et al. (2014)
MS
Photosystem II
reaction center
Pisum sativum,
thylakoids
α
2 × 17
550 kDa Native, CHAPS or
DDM
A8-35
A. Zehetner & H. Scheer, unpublished observation cited in Popot
et al. (2003)
Photosystem II
reaction center
Thermosynechococcus elongatus,
thylakoids
α
2 × 20 +
cofactors
450 kDa Native, DDM
A8-35
Nowaczyk et al. (2004)
Maltose
transporter
E. coli,
plasma
membrane
α
1
150 kDa Native, OG, or
direct extraction
with A8-35
A8-35
M. Zoonens & H.A. Shuman, unpublished observations cited
in Popot et al. (2003)
Photochemistry
SEC, oxygen evolution
ATPase activity
240
5 Formation and Properties of Membrane Protein/Amphipol Complexes
reaction center
Rhodobacter
sphaeroides,
plasma
membrane
α
3
96 kDa
Native, Hecameg
A8-35
SG-AUC
Tribet et al. (1996)
A8-75
SG-AUC
Tribet et al. (1996, 1997)
A34-35,
A34-75
SG-AUC
Tribet et al. (1996)
Native, plasma
membrane
SMA
Spectrum, photochemistry
Swainsbury et al. (2014)
Glycophorin A
transmembrane
anchor (tGpA),
monomer or dimer
Human, plasma
membrane
α
1 or 2
~4 or
~8 kDa
Synthetic peptide,
urea
A5-75
CD
Gohon (1996) and Popot et al. (2003)
Synthetic peptides,
TFE
A8-35
CD, FRET, sequencespecific dimerization
Stangl et al. (2014)
F
1 F
o ATP
synthase
E. coli, plasma
membrane
α
21–24
~560
kDa
Native,
taurodeoxycholate
A8-75
NS-EM
Wilkens and Capaldi (1998a,b), Wilkens (2000), and Wilkens et al.
(2000)
Sarcoplasmic
calcium pump
(SERCA1a)
Oryctolagus
cuniculus,
sarcoplasmic
reticulum
α
1
110 kDa Native, DDM
A8-35
ATPase activity, SEC,
SV-AUC
Champeil et al. (2000) and Picard et al. (2006)
SAPol
ATPase activity, SEC
Picard et al. (2006)
PMAL-C12
e
,
PMALA-C12
ATPase activity,
SEC
Picard et al. (2006)
Diacylglycerol
kinase (DAGK)
E. coli,
plasma
membrane
α
3 × 1
40 kDa Native, Empigen
or DM
OAPA-20
e
,
PMAL-B-50,
PMAL-B-100
e
SDS-PAGE after chemical
cross-linking, ATPase
activity, MS
Nagy et al. (2001), Gorzelle et al. (2002), and Hopper et al. (2013)
Photosystem I
Synechocystis
PCC 6803,
thylakoids
α
3 × 12 +
> 120
cofactors
> 1 MDa Native, DDM
A8-35
Electrochemistry
Kievit and Brudvig (2001)
Nicotinic
acetylcholine
receptor (nAChR)
Torpedo
marmorata,
plasma
membrane
α
2 × 5
535 kDa Native, CHAPS
A8-35
Allosteric transitions, ligand
and toxin binding
Martinez et al. (2002) and Charvolin et al. (2009)
Rhodopsin
Bos taurus,
retina discs
α
1 + retinal
40 kDa
Native, CHAPS
A8-35
Transition to meta-II state
C. Tribet, C. Creuzenet & JLP, unpublished observations cited in
Popot et al. (2003)
Native, DDM
NS-EM (rhodopsin/GRK5
complex)
He et al. (2017)
Protein(s)
(abbreviation)
Organism,
membrane
Structural
type a
Number
of
chains
Overall
mass
State and
environment before
trapping
Amphipol b
Evidence for native state
after trapping
References
Cytochrome bc
1
B. taurus, inner
mitochondrial
membrane
α
2 × 11
490 kDa Native, DDM
A8-35
NAPols
Bechara et al. (2012)
Catalysis of electron transfer,
SEC, SG-AUC, SAXS
Popot et al. (2003), Charvolin et al. (2009), Charvolin et al. (2014),
and Ferrandez et al. (2014)
MS
Photosystem II
reaction center
Pisum sativum,
thylakoids
α
2 × 17
550 kDa Native, CHAPS or
DDM
A8-35
A. Zehetner & H. Scheer, unpublished observation cited in Popot
et al. (2003)
Photosystem II
reaction center
Thermosynechococcus elongatus,
thylakoids
α
2 × 20 +
cofactors
450 kDa Native, DDM
A8-35
Nowaczyk et al. (2004)
Maltose
transporter
E. coli,
plasma
membrane
α
1
150 kDa Native, OG, or
direct extraction
with A8-35
A8-35
M. Zoonens & H.A. Shuman, unpublished observations cited
in Popot et al. (2003)
Photochemistry
SEC, oxygen evolution
ATPase activity
240
5 Formation and Properties of Membrane Protein/Amphipol Complexes