Table 4.5
(continued)
Type of modification
Amphipol modified (short
name of derivative)
Applications
a
References
b
Tags, adjuvants
Biotin
A8-35 (BAPol)
Immobilizing MPs onto chips or beads for SPR or
fluorescence measurements. See Chap. 13,
§§ 13.2.1 and
13.2.2
Charvolin et al. (2009), Della Pia et al. (2014a, b),
and Ferrandez et al. (2014)
Selecting soluble protein binders against immobilized
MPs. See Chap. 13,
§ 13.2.3
Ferrandez et al. (2014)
Visualizing the APol belt in EM using monovalent avidin.
See Chap. 13,
§ 13.3
Perry et al. (2018)
Polyhistidine
A8-35 (HistAPol)
Reversibly immobilizing MPs onto Ni
2+
- or Co
2+
-bearing
supports. See Chap. 13,
§ 13.2.4
Giusti et al. (2015)
Randomly distributed
imidazole moieties
A8-35 (ImidAPol)
Reversibly immobilizing MPs onto Ni
2+
- or Co
2+
-bearing
supports. See Chap. 13,
§ 13.2.4
Unpublished data cited in Le Bon et al. (2014a)
Oligodeoxynucleotide
A8-35 (OligAPol)
Immobilizing MPs onto DNA chips. See Chap. 13,
§
13.2.4
Le Bon et al. (2014a)
Vaccination, using the oligodeoxynucleotide as an
adjuvant
Thiomorpholine
A8-35 (SulfidAPol)
Immobilization of MPs onto gold surfaces, e.g. for surface
plasmon resonance or surface-enhanced resonance
spectroscopy. See Chap. 13,
§ 13.2.4
Unpublished data
Attaching gold beads to APol belts for EM applications.
See Chap. 13,
§ 13.3
Peptide EP67
A8-35 (EP67-A8-35)
Vaccination, using the peptide as an adjuvant
Tifrea et al. (2018b)
Resiquimod
A8-35 (ResiqAPol)
Vaccination, using Resiquimod as an adjuvant
Tifrea et al. (2018a)
Miscellaneous
Azobenzene
A8-75
Photostimulated permeabilization of lipid vesicles and
protein renaturation
Sebai et al. (2010, 2012) and Martin et al. (2015)
4-Carboxytempo
A8-35 (SpinAPol)
Electron paramagnetic resonance, NMR, and
fluorescence
spectroscopy applications; protection against free
radicals?
Unpublished data
N-Methylation
MethylAPol
A version of A8-35 carrying methylamide- rather than
amide-linked side chains, for use in FTIR and other
infrared spectroscopic studies
Unpublished data
Updated from Le Bon et al. (2014b)
a
Most of the applications listed have been validated in one or more of the studies whose references are given. A few of them are only contemplated or being explored
b
References to synthesis and applications
200
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols
(continued)
Type of modification
Amphipol modified (short
name of derivative)
Applications
a
References
b
Tags, adjuvants
Biotin
A8-35 (BAPol)
Immobilizing MPs onto chips or beads for SPR or
fluorescence measurements. See Chap. 13,
§§ 13.2.1 and
13.2.2
Charvolin et al. (2009), Della Pia et al. (2014a, b),
and Ferrandez et al. (2014)
Selecting soluble protein binders against immobilized
MPs. See Chap. 13,
§ 13.2.3
Ferrandez et al. (2014)
Visualizing the APol belt in EM using monovalent avidin.
See Chap. 13,
§ 13.3
Perry et al. (2018)
Polyhistidine
A8-35 (HistAPol)
Reversibly immobilizing MPs onto Ni
2+
- or Co
2+
-bearing
supports. See Chap. 13,
§ 13.2.4
Giusti et al. (2015)
Randomly distributed
imidazole moieties
A8-35 (ImidAPol)
Reversibly immobilizing MPs onto Ni
2+
- or Co
2+
-bearing
supports. See Chap. 13,
§ 13.2.4
Unpublished data cited in Le Bon et al. (2014a)
Oligodeoxynucleotide
A8-35 (OligAPol)
Immobilizing MPs onto DNA chips. See Chap. 13,
§
13.2.4
Le Bon et al. (2014a)
Vaccination, using the oligodeoxynucleotide as an
adjuvant
Thiomorpholine
A8-35 (SulfidAPol)
Immobilization of MPs onto gold surfaces, e.g. for surface
plasmon resonance or surface-enhanced resonance
spectroscopy. See Chap. 13,
§ 13.2.4
Unpublished data
Attaching gold beads to APol belts for EM applications.
See Chap. 13,
§ 13.3
Peptide EP67
A8-35 (EP67-A8-35)
Vaccination, using the peptide as an adjuvant
Tifrea et al. (2018b)
Resiquimod
A8-35 (ResiqAPol)
Vaccination, using Resiquimod as an adjuvant
Tifrea et al. (2018a)
Miscellaneous
Azobenzene
A8-75
Photostimulated permeabilization of lipid vesicles and
protein renaturation
Sebai et al. (2010, 2012) and Martin et al. (2015)
4-Carboxytempo
A8-35 (SpinAPol)
Electron paramagnetic resonance, NMR, and
fluorescence
spectroscopy applications; protection against free
radicals?
Unpublished data
N-Methylation
MethylAPol
A version of A8-35 carrying methylamide- rather than
amide-linked side chains, for use in FTIR and other
infrared spectroscopic studies
Unpublished data
Updated from Le Bon et al. (2014b)
a
Most of the applications listed have been validated in one or more of the studies whose references are given. A few of them are only contemplated or being explored
b
References to synthesis and applications
200
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols