Table 4.5
Tagged and labeled derivatives of A8-35 and A8-75 and their uses.
Type of modification
Amphipol modified (short
name of derivative)
Applications
a
References
b
Isotopic labeling
14
C
A8-75
Following A8-75 distribution and exchange
Tribet et al. (1997)
3
H
A8-35
Following A8-35 distribution
Gohon et al. (2008) and Charvolin et al. (2014)
Evaluating MP/A8-35 mass ratio in complexes
2
H (on side chains)
A8-35 (DAPol)
Eliminating the contribution of side-chain protons for
NMR, EINS, or QENS measurements
Gohon et al. (2004, 2006, 2008), Zoonens et al. (2005),
Catoire et al. (2009, 2010a), Etzkorn et al. (2014),
Planchard et al. (2014), Sverzhinsky et al. (2014),
and Tehei et al. (2014)
Contrast-matching in SANS, AUC
2
H (perdeuteration)
A8-35 (perDAPol)
Eliminating protons for NMR measurements
Giusti et al. (2014)
Contrast-matching in SANS, AUC
Fluorescent labeling (λ
exc ,
λ em )
Naphthalene
(290 nm/310–370 nm)
A8-75
Studying the interactions of A8-75 with lipid vesicles
Vial et al. (2005, 2009)
NBD
(470 nm/530 nm)
A8-35 (FAPol
NBD )
Studying A8-35 distribution, binding, and exchange
Zoonens et al. (2007), Giusti et al. (2012), Le Bon et al.
(2014a), and Sverzhinsky et al. (2014)
Determining the CAC of A8-35 by FRET with FAPol
rhod
Alexa Fluor 488
(490 nm/525 nm)
A8-35 (FAPol
AF488 )
Unpublished data
Unpublished data
Fluorescein
(495 nm/540 nm)
A8-35(FAPol
fluo )
Mapping the transmembrane region of a MP by FRET
with single-tryptophan mutants
Opačić et al. (2014b)
Rhodamine
(555 nm/575 nm)
A8-35 (FAPol
rhod )
Examining the distribution of A8-35 upon delivery of a
transmembrane peptide to cells in culture
Giusti et al. (2012) and Fernandez et al. (2014)
Determining the CAC of A8-35 by FRET with FAPol
NBD
Following the biodistribution of APols in mice
Atto 647
(651 nm/667 nm)
A8-35 (FAPol
A647 )
Following in vivo the distribution and elimination of
A8-35 in mice
Unpublished results; see Chap. 15.
Alexa Fluor 647
(651 nm/668 nm)
A8-35 (FAPol
AF647 )
Following in vivo distribution and elimination of A8-35 in
mice
Della Pia et al. (2014a, b), Fernandez et al. (2014), and
Le Bon et al. (2014a)
Tracer in APol-mediated immobilization of MPs
(continued)
4.4 Labeled and Functionalized Amphipols
199
Tagged and labeled derivatives of A8-35 and A8-75 and their uses.
Type of modification
Amphipol modified (short
name of derivative)
Applications
a
References
b
Isotopic labeling
14
C
A8-75
Following A8-75 distribution and exchange
Tribet et al. (1997)
3
H
A8-35
Following A8-35 distribution
Gohon et al. (2008) and Charvolin et al. (2014)
Evaluating MP/A8-35 mass ratio in complexes
2
H (on side chains)
A8-35 (DAPol)
Eliminating the contribution of side-chain protons for
NMR, EINS, or QENS measurements
Gohon et al. (2004, 2006, 2008), Zoonens et al. (2005),
Catoire et al. (2009, 2010a), Etzkorn et al. (2014),
Planchard et al. (2014), Sverzhinsky et al. (2014),
and Tehei et al. (2014)
Contrast-matching in SANS, AUC
2
H (perdeuteration)
A8-35 (perDAPol)
Eliminating protons for NMR measurements
Giusti et al. (2014)
Contrast-matching in SANS, AUC
Fluorescent labeling (λ
exc ,
λ em )
Naphthalene
(290 nm/310–370 nm)
A8-75
Studying the interactions of A8-75 with lipid vesicles
Vial et al. (2005, 2009)
NBD
(470 nm/530 nm)
A8-35 (FAPol
NBD )
Studying A8-35 distribution, binding, and exchange
Zoonens et al. (2007), Giusti et al. (2012), Le Bon et al.
(2014a), and Sverzhinsky et al. (2014)
Determining the CAC of A8-35 by FRET with FAPol
rhod
Alexa Fluor 488
(490 nm/525 nm)
A8-35 (FAPol
AF488 )
Unpublished data
Unpublished data
Fluorescein
(495 nm/540 nm)
A8-35(FAPol
fluo )
Mapping the transmembrane region of a MP by FRET
with single-tryptophan mutants
Opačić et al. (2014b)
Rhodamine
(555 nm/575 nm)
A8-35 (FAPol
rhod )
Examining the distribution of A8-35 upon delivery of a
transmembrane peptide to cells in culture
Giusti et al. (2012) and Fernandez et al. (2014)
Determining the CAC of A8-35 by FRET with FAPol
NBD
Following the biodistribution of APols in mice
Atto 647
(651 nm/667 nm)
A8-35 (FAPol
A647 )
Following in vivo the distribution and elimination of
A8-35 in mice
Unpublished results; see Chap. 15.
Alexa Fluor 647
(651 nm/668 nm)
A8-35 (FAPol
AF647 )
Following in vivo distribution and elimination of A8-35 in
mice
Della Pia et al. (2014a, b), Fernandez et al. (2014), and
Le Bon et al. (2014a)
Tracer in APol-mediated immobilization of MPs
(continued)
4.4 Labeled and Functionalized Amphipols
199