Table 4.2
(continued)
4.11
FRET
4.12
4.13
Tensiometry
4.14
4.15
EPR
4.16 Sulfonated APols
(SAPols)
Synthesis and properties of APols analogous to A8-35, in which the
isopropyl groups are replaced by taurine.
4.17 NVoy = NV10
Coupled SEC,
SLS, and RI
measurements
NVoy, a commercial amphiphilic derivative of polyfructose (see
scheme in Fig. 4.5b), was used to keep soluble MPs produced by
cell-free synthesis (see Chap. 7). The particles were studied by
SEC and SLS.
4.18 A8-35
MD
4.19
4.20
Study
Amphipol(s)
Description
of synthesis
Characterization of amphipol particle solution properties by:
Comments
References
SANS SG-AUC SV-AUC Eq-AUC SEC DLS SLS EM NMR Other methods
A8-35 and
fluorescently labeled
A8-35
(FAPol
NBD )
A8-35,
DAPol,
and [
3
H]A8-35
Heteropolymeric
glucose-based
non-ionic APols
(NAPols)
Glucosylated NA
Pols similar to those
in Study 4.13
Styrene-maleic
acid copolymer
(SMA)
Homopolymeric
glucose-based
NAPols
A8-35
and
fluorescent versions
thereof
(FAPol
NBD and
FAPol
rhod )
Densimetry,
tensiometry
Tensiometry,
FRET
The formation, size, and stability of A8-35
particles and MP/A8-35
complexes were studied in the presence or absence of detergent or
excess APols, providing insights into the rate of exchange of
surfactants between particles.
In the course of a study of bacteriorhodopsin (BR)/A8-35 complexes,
further data were collected on the behavior of A8-35
particles. The
techniques listed are those relevant to these particles.
First-generation glucose-based NAPols were obtained by
co-telomerization of hydrophilic and amphipathic monomers.
Glucosylated NAPols similar to those in Study 4.13 were obtained by
grafting hydrophobic chains onto a hydrophilic homopolymer.
The size of SMA/DMPC complexes (SMALPs) was studied by DLS
and EM after negative staining and their organization by solution
NMR and EPR. The structure of SMA is given in Fig. 4.1e and a
scheme of the SMALP particles in Fig. 4.27a.
The critical aggregation concentration (CAC) of A8-35
was
determined both by tensiometry and by FRET using two
complementary fluorescent derivatives of A8-35.
Homopolymeric glucose-based NAPols were obtained by
telomerization of an amphipathic precursor. Their structure is shown
in Fig. 4.5d.
The structure and dynamics of A8-35
particles were studied by
all-atom and coarse-grained molecular dynamics simulations.
Zoonens et al.
(2007)
Gohon et al. (2008)
Sharma et al. (2008)
Bazzacco et al.
(2009)
Knowles et al.
(2009),
Orwick et al.
(2012)
Dahmane et al.
(2011)
Guild et al. (2011),
Klammt et al.
(2011)
Perlmutter et al.
(2011)
Bazzacco et al.
(2012),
Sharma et al. (2012)
Giusti et al. (2012)
(continued)
172
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols
(continued)
4.11
FRET
4.12
4.13
Tensiometry
4.14
4.15
EPR
4.16 Sulfonated APols
(SAPols)
Synthesis and properties of APols analogous to A8-35, in which the
isopropyl groups are replaced by taurine.
4.17 NVoy = NV10
Coupled SEC,
SLS, and RI
measurements
NVoy, a commercial amphiphilic derivative of polyfructose (see
scheme in Fig. 4.5b), was used to keep soluble MPs produced by
cell-free synthesis (see Chap. 7). The particles were studied by
SEC and SLS.
4.18 A8-35
MD
4.19
4.20
Study
Amphipol(s)
Description
of synthesis
Characterization of amphipol particle solution properties by:
Comments
References
SANS SG-AUC SV-AUC Eq-AUC SEC DLS SLS EM NMR Other methods
A8-35 and
fluorescently labeled
A8-35
(FAPol
NBD )
A8-35,
DAPol,
and [
3
H]A8-35
Heteropolymeric
glucose-based
non-ionic APols
(NAPols)
Glucosylated NA
Pols similar to those
in Study 4.13
Styrene-maleic
acid copolymer
(SMA)
Homopolymeric
glucose-based
NAPols
A8-35
and
fluorescent versions
thereof
(FAPol
NBD and
FAPol
rhod )
Densimetry,
tensiometry
Tensiometry,
FRET
The formation, size, and stability of A8-35
particles and MP/A8-35
complexes were studied in the presence or absence of detergent or
excess APols, providing insights into the rate of exchange of
surfactants between particles.
In the course of a study of bacteriorhodopsin (BR)/A8-35 complexes,
further data were collected on the behavior of A8-35
particles. The
techniques listed are those relevant to these particles.
First-generation glucose-based NAPols were obtained by
co-telomerization of hydrophilic and amphipathic monomers.
Glucosylated NAPols similar to those in Study 4.13 were obtained by
grafting hydrophobic chains onto a hydrophilic homopolymer.
The size of SMA/DMPC complexes (SMALPs) was studied by DLS
and EM after negative staining and their organization by solution
NMR and EPR. The structure of SMA is given in Fig. 4.1e and a
scheme of the SMALP particles in Fig. 4.27a.
The critical aggregation concentration (CAC) of A8-35
was
determined both by tensiometry and by FRET using two
complementary fluorescent derivatives of A8-35.
Homopolymeric glucose-based NAPols were obtained by
telomerization of an amphipathic precursor. Their structure is shown
in Fig. 4.5d.
The structure and dynamics of A8-35
particles were studied by
all-atom and coarse-grained molecular dynamics simulations.
Zoonens et al.
(2007)
Gohon et al. (2008)
Sharma et al. (2008)
Bazzacco et al.
(2009)
Knowles et al.
(2009),
Orwick et al.
(2012)
Dahmane et al.
(2011)
Guild et al. (2011),
Klammt et al.
(2011)
Perlmutter et al.
(2011)
Bazzacco et al.
(2012),
Sharma et al. (2012)
Giusti et al. (2012)
(continued)
172
4 Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols