Another way to produce MP/ND complexes is to use NDs as the host surfactant during MP
in vitro cell-free synthesis (see e.g. Cappuccio et al. 2008; Katzen et al. 2008; Yang et al. 2011; Gao
et al. 2012; Roos et al. 2012; Proverbio et al. 2013; Henrich et al. 2015, 2016, 2017; Rues et al. 2016).
Finally, a few cases have been described where the target MP was directly folded in NDs from a
mixture of MP, MSPs, and lipids in SDS (Etzkorn et al. 2013; Shenkarev et al. 2013) (see below).
3.3.5
Nanodisc-Based Investigations of Membrane Proteins
ND-trapped MPs can be studied by most of the methods applicable to MP/detergent complexes, with
the double advantage of increased stability and the presence of a lipid environment. Table 3.1 offers a
selection of examples.
In discussing – briefly – the applications of NDs to MP structural and functional studies, one may
perhaps consider three cases of figures: (i) situations where NDs have a clear edge over all or most of
Table 3.1 A selection of works combining the use of nanodiscs with various biophysical or biochemical
methodologies for producing and/or studying membrane proteins.
Methodology
References
NMR
Kijac et al. (2007), Shenkarev et al. (2010), Park
et al. (2011a), Qureshi and Goto (2011),
Warschawski et al. ( 2011), Etzkorn et al. (2013),
Hagn et al. 2013), Tzitzilonis et al. (2013), Malhotra
and Alder (2014), Hagn and Wagner (2015),
Kucharska et al. (2015), Mineev et al. (2015),
Morgado et al. (2015), Casiraghi et al. (2016),
Viegas et al. (2016), Mineev and Nadezhdin (2017),
Nasr et al. (2017), and Puthenveetil et al. (2017)
EPR
Shin et al. (2014), Alvarez et al. (2015), Kang et al.
(2015), and Georgieva (2017)
Absorbance, fluorescence, and vibrational optical
spectroscopies
Denisov et al. (2007), Ranaghan et al. (2011),
Tsukamoto et al. (2011), Taufik et al. (2013);
Johnson et al. (2014), Shin et al. (2014), Mak et al.
(2015), and Zoghbi and Altenberg (2017)
SANS and SAXS, neutron reflectivity
Bayburt et al. (2006), Wadsäter et al. (2012),
Periasamy et al. (2013), and Skar-Gislinge et al.
(2015)
Analytical ultracentrifugation
Inagaki and Ghirlando (2017)
Electron microscopy
Katayama et al. (2010), Ye et al. (2010), Frauenfeld
et al. (2011), Gogol et al. (2013), Xu et al. (2013),
Akkaladevi et al. (2015), Efremov et al. (2015),
Grushin et al. (2015), Zhang et al. (2015), Daury
et al. (2016), Gao et al. (2016), Gatsogiannis et al.
(2016), Kedrov et al. (2016), Kumar et al. (2016),
Lee et al. (2016), Matthies et al. (2016), Shen et al.
(2016), and Nasr et al. (2017)
Mass spectrometry
Hopper et al. (2013), Landreh and Robinson (2015),
Marty et al. (2016), and Henrich et al. (2017)
Immobilization onto solid supports, surface
plasmon resonance and single-molecule studies,
NMR-based drug screening
Bayburt and Sligar (2002), Shaw et al. (2007), Nath
et al. (2008), Früh et al. (2011), Ritchie et al. (2011),
Wadsäter et al. (2012), Laursen et al. (2014),
Gillette et al. (2015), Lamichhane et al. (2015), and
Hansen et al. (2016)
(continued)
3.3 Nanodiscs
115
in vitro cell-free synthesis (see e.g. Cappuccio et al. 2008; Katzen et al. 2008; Yang et al. 2011; Gao
et al. 2012; Roos et al. 2012; Proverbio et al. 2013; Henrich et al. 2015, 2016, 2017; Rues et al. 2016).
Finally, a few cases have been described where the target MP was directly folded in NDs from a
mixture of MP, MSPs, and lipids in SDS (Etzkorn et al. 2013; Shenkarev et al. 2013) (see below).
3.3.5
Nanodisc-Based Investigations of Membrane Proteins
ND-trapped MPs can be studied by most of the methods applicable to MP/detergent complexes, with
the double advantage of increased stability and the presence of a lipid environment. Table 3.1 offers a
selection of examples.
In discussing – briefly – the applications of NDs to MP structural and functional studies, one may
perhaps consider three cases of figures: (i) situations where NDs have a clear edge over all or most of
Table 3.1 A selection of works combining the use of nanodiscs with various biophysical or biochemical
methodologies for producing and/or studying membrane proteins.
Methodology
References
NMR
Kijac et al. (2007), Shenkarev et al. (2010), Park
et al. (2011a), Qureshi and Goto (2011),
Warschawski et al. ( 2011), Etzkorn et al. (2013),
Hagn et al. 2013), Tzitzilonis et al. (2013), Malhotra
and Alder (2014), Hagn and Wagner (2015),
Kucharska et al. (2015), Mineev et al. (2015),
Morgado et al. (2015), Casiraghi et al. (2016),
Viegas et al. (2016), Mineev and Nadezhdin (2017),
Nasr et al. (2017), and Puthenveetil et al. (2017)
EPR
Shin et al. (2014), Alvarez et al. (2015), Kang et al.
(2015), and Georgieva (2017)
Absorbance, fluorescence, and vibrational optical
spectroscopies
Denisov et al. (2007), Ranaghan et al. (2011),
Tsukamoto et al. (2011), Taufik et al. (2013);
Johnson et al. (2014), Shin et al. (2014), Mak et al.
(2015), and Zoghbi and Altenberg (2017)
SANS and SAXS, neutron reflectivity
Bayburt et al. (2006), Wadsäter et al. (2012),
Periasamy et al. (2013), and Skar-Gislinge et al.
(2015)
Analytical ultracentrifugation
Inagaki and Ghirlando (2017)
Electron microscopy
Katayama et al. (2010), Ye et al. (2010), Frauenfeld
et al. (2011), Gogol et al. (2013), Xu et al. (2013),
Akkaladevi et al. (2015), Efremov et al. (2015),
Grushin et al. (2015), Zhang et al. (2015), Daury
et al. (2016), Gao et al. (2016), Gatsogiannis et al.
(2016), Kedrov et al. (2016), Kumar et al. (2016),
Lee et al. (2016), Matthies et al. (2016), Shen et al.
(2016), and Nasr et al. (2017)
Mass spectrometry
Hopper et al. (2013), Landreh and Robinson (2015),
Marty et al. (2016), and Henrich et al. (2017)
Immobilization onto solid supports, surface
plasmon resonance and single-molecule studies,
NMR-based drug screening
Bayburt and Sligar (2002), Shaw et al. (2007), Nath
et al. (2008), Früh et al. (2011), Ritchie et al. (2011),
Wadsäter et al. (2012), Laursen et al. (2014),
Gillette et al. (2015), Lamichhane et al. (2015), and
Hansen et al. (2016)
(continued)
3.3 Nanodiscs
115