the interface of evanescent wave with the same wavelength as the incident light
[14]. The evanescent wave exponentially decays with a characteristic decay length of
~100 nm [15]. Hence, the evanescent wave illuminates the thin region relatively
close to the surface, as compared with the epi-illumination, where the light goes
deeply into the water phase (Panel b). As a result, the number of fluorophores excited
Fig. 3.21 Real time GP imaging of giant liposome of DMPC/DMPE binary mixture
Giant liposome of DMPC/DPPC (1:1) binary mixture was prepared by gentle hydration method in
aqueous solution containing 2 mM CaCl 2 .The liposome was observed by GP imaging microscopic
instrument in changing temperature
Video is available. (with kind permission of © Kazuo Ohki 2005. All Rights Reserved)
3.7 Fluorescence Method
51
[14]. The evanescent wave exponentially decays with a characteristic decay length of
~100 nm [15]. Hence, the evanescent wave illuminates the thin region relatively
close to the surface, as compared with the epi-illumination, where the light goes
deeply into the water phase (Panel b). As a result, the number of fluorophores excited
Fig. 3.21 Real time GP imaging of giant liposome of DMPC/DMPE binary mixture
Giant liposome of DMPC/DPPC (1:1) binary mixture was prepared by gentle hydration method in
aqueous solution containing 2 mM CaCl 2 .The liposome was observed by GP imaging microscopic
instrument in changing temperature
Video is available. (with kind permission of © Kazuo Ohki 2005. All Rights Reserved)
3.7 Fluorescence Method
51