51. Hu X, Xiao C, Huang Z, Zhang Z, Li Z (2000) Pilot production of u-PA with porous
microcarrier cell culture. Cytotechnology 33:13–19
52. Kumar A, Bansal V, Nandakumar KS, Galaev IY, Roychourhury PK, Holmdahl R,
Mattiasson B (2006) Integrated bioprocess for the production and isolation of urokinase
from animal cell culture using supermacroporous cryogel matrices. Biotechnol Bioeng
93:636–646
53. Nilsang S, Nandakumar KS, Galaev IY, Rakshit SK, Holmdahl R, Mattiasson B, Kumar A
(2007) Monoclonal antibody production using a new supermacroporous cryogel bioreactor.
Biotechnol Prog 23:932–939
54. Arvidsson P, Plieva FM, Savina IN, Lozinsky VI, Fexby S, Bu ¨low L, Galaev IY, Mattiasson B
(2002) Chromatography of microbial cells using continuous supermacroporous affinity and
ion-exchange columns. J Chromatogr A 977:27–38
55. Daniak MB, Plieva FM, Galaev IY, Hatti-Kaul R, Mattiasson B (2005) Cell chromatography:
separation of different microbial cells using IMAC supermacroporous monolithic columns.
Biotechnol Progr 21:644–6649
56. Kumar A, Plieva FM, Galaev IY, Mattiasson B (2003) Affinity fractionation of lymphocytes
using a monolithic cryogel. J Immunol Methods 283:185–194
57. Dainiak MB, Kumar A, Galaev IY, Mattiasson B (2006) Detachment of affinity-captured
bioparticles by elastic deformation of a macroporous hydrogel. Proc Natl Acad Sci USA
103:849–854
58. Galaev IY, Dainiak MB, Plieva FM, Mattiasson B (2007) Effect of matrix elasticity on
affinity binding and release of bioparticles. Elution of bound cells by temperature-induced
shrinkage of the smart macroporous hydrogel. Langmuir 23:35–40
59. Tellum M, Hansson MJ, Dainiak MB, Ma ˚nsson R, Surve S, Elme ´r E, O ¨ nnerfjord P,
Mattiasson G (2006) Binding mitochondria to cryogel monoliths allows detection of proteins
specially released following permeability transition. Anal Biochem 348:209–221
60. Ahlqvist J, Kumar A, Sundstro ¨m H, Ledung E, Ho ¨rnsten EG, Enfors S-O, Mattiasson B
(2006) Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using
labeling with specific antibodies. J Biotechnol 122:216–225
61. Noppe W, Plieva F, Galaev IY, Mattiasson B, Deckmyn H (2006) Immobilized peptide
displaying phages as affinity ligands. Purification of lactoferrin from defatted milk.
J Chromatogr 1101:79–85
62. Noppe W, Plieva F, Vanhoorelbeke K, Deckmyn H, Tuncel M, Tuncel A, Galaev IY,
Mattiasson B (2007) Macroporous monolithic gels, cryogels, with immobilized phages
from phage-display library as a new platform for fat development of affinity adsorbent
capable of target capture from crude feeds. J Biotechnol 131:293–299
63. Noppe W, Plieva F, Galaev IY, Pottel H, Deckmyn H, Mattiasson B (2009) Chromatopanning: an efficient new mode of identifying suitable ligands from phage display libraries.
BMC Biotechnol 9:21
64. Kovac K, Gutie ´rrez-AguirreI BM, PeterkaM P-PM, RavnikarM ZMJ, SchultzAC RP (2009)
A novel method for concentrating hepatitis A virus and caliciviruses from bottled water.
J VirolMethods 162:272–275
65. Plieva FM, Savina IN, Deraz S, Andersson J, Galaev IY, Mattiasson B (2004) Characterization
of supermacroporous monolithic polyacrylamide based matrices designed for chromatography
of bioparticles. J Chromatogr B 807:129–137
66. Savina IN, Galaev IY, Mattiasson B (2005) Anion-exchange supermacroporous monolithic
matrices with grafted polymer brushes of N, N-dimethylaminoethylmethacrylate.
J Chromatogr A 1092:199–205
67. Savina IN, Mattiasson B, Galaev IY (2005) Graft polymerization of acrylic acid onto
macroporous polyacrylamide gel (cryogel) by potassium diperiodateocuprate. Polymer 46:
9596–9603
278
B. Mattiasson
microcarrier cell culture. Cytotechnology 33:13–19
52. Kumar A, Bansal V, Nandakumar KS, Galaev IY, Roychourhury PK, Holmdahl R,
Mattiasson B (2006) Integrated bioprocess for the production and isolation of urokinase
from animal cell culture using supermacroporous cryogel matrices. Biotechnol Bioeng
93:636–646
53. Nilsang S, Nandakumar KS, Galaev IY, Rakshit SK, Holmdahl R, Mattiasson B, Kumar A
(2007) Monoclonal antibody production using a new supermacroporous cryogel bioreactor.
Biotechnol Prog 23:932–939
54. Arvidsson P, Plieva FM, Savina IN, Lozinsky VI, Fexby S, Bu ¨low L, Galaev IY, Mattiasson B
(2002) Chromatography of microbial cells using continuous supermacroporous affinity and
ion-exchange columns. J Chromatogr A 977:27–38
55. Daniak MB, Plieva FM, Galaev IY, Hatti-Kaul R, Mattiasson B (2005) Cell chromatography:
separation of different microbial cells using IMAC supermacroporous monolithic columns.
Biotechnol Progr 21:644–6649
56. Kumar A, Plieva FM, Galaev IY, Mattiasson B (2003) Affinity fractionation of lymphocytes
using a monolithic cryogel. J Immunol Methods 283:185–194
57. Dainiak MB, Kumar A, Galaev IY, Mattiasson B (2006) Detachment of affinity-captured
bioparticles by elastic deformation of a macroporous hydrogel. Proc Natl Acad Sci USA
103:849–854
58. Galaev IY, Dainiak MB, Plieva FM, Mattiasson B (2007) Effect of matrix elasticity on
affinity binding and release of bioparticles. Elution of bound cells by temperature-induced
shrinkage of the smart macroporous hydrogel. Langmuir 23:35–40
59. Tellum M, Hansson MJ, Dainiak MB, Ma ˚nsson R, Surve S, Elme ´r E, O ¨ nnerfjord P,
Mattiasson G (2006) Binding mitochondria to cryogel monoliths allows detection of proteins
specially released following permeability transition. Anal Biochem 348:209–221
60. Ahlqvist J, Kumar A, Sundstro ¨m H, Ledung E, Ho ¨rnsten EG, Enfors S-O, Mattiasson B
(2006) Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using
labeling with specific antibodies. J Biotechnol 122:216–225
61. Noppe W, Plieva F, Galaev IY, Mattiasson B, Deckmyn H (2006) Immobilized peptide
displaying phages as affinity ligands. Purification of lactoferrin from defatted milk.
J Chromatogr 1101:79–85
62. Noppe W, Plieva F, Vanhoorelbeke K, Deckmyn H, Tuncel M, Tuncel A, Galaev IY,
Mattiasson B (2007) Macroporous monolithic gels, cryogels, with immobilized phages
from phage-display library as a new platform for fat development of affinity adsorbent
capable of target capture from crude feeds. J Biotechnol 131:293–299
63. Noppe W, Plieva F, Galaev IY, Pottel H, Deckmyn H, Mattiasson B (2009) Chromatopanning: an efficient new mode of identifying suitable ligands from phage display libraries.
BMC Biotechnol 9:21
64. Kovac K, Gutie ´rrez-AguirreI BM, PeterkaM P-PM, RavnikarM ZMJ, SchultzAC RP (2009)
A novel method for concentrating hepatitis A virus and caliciviruses from bottled water.
J VirolMethods 162:272–275
65. Plieva FM, Savina IN, Deraz S, Andersson J, Galaev IY, Mattiasson B (2004) Characterization
of supermacroporous monolithic polyacrylamide based matrices designed for chromatography
of bioparticles. J Chromatogr B 807:129–137
66. Savina IN, Galaev IY, Mattiasson B (2005) Anion-exchange supermacroporous monolithic
matrices with grafted polymer brushes of N, N-dimethylaminoethylmethacrylate.
J Chromatogr A 1092:199–205
67. Savina IN, Mattiasson B, Galaev IY (2005) Graft polymerization of acrylic acid onto
macroporous polyacrylamide gel (cryogel) by potassium diperiodateocuprate. Polymer 46:
9596–9603
278
B. Mattiasson