32. Kuyukina MS, Ivshina IB, Kamenskikh TN, Bulicheva AV, Stukova GI (2013) Survival of
cryogel-immobilized Rhodococcus strains in crude oil-contaminated soil and their impact on
biodegradation efficiency. Int Biodeter Biodegr 84:118–125
33. Cunningham CJ, Ivshina IB, Lozinsky VI, Kuyukina MS, Philp JC (2004) Bioremediation of
diesel-contaminated soil by microorganisms immobilized in polyvinyl alcohol. Int Biodeter
Biodegr 54:167–174
34. Siripattanakul S, Wirojanagud W, McEvoy J, Khan E (2008) Effect of cell-to-matrix ratio in
polyvinyl alcohol immobilized pure and mixed cultures on atrazine degradation. Water Air
Soil Pollut 8:257–266
35. Rainina EI, Badalian IE, Ignatov OV, Fedorov AY, Simonian AL, Varfolomeyev SD (1996)
Cell biosensor for detection of phenol in aqueous solutions. Appl Biochem Biotechnol
56:117–127
36. Simonian AL, Rainina EI, Lozinsky VI, Badalian IE, Khachatrian GE, Tatikian SS,
Makhlis TA, Vorfolomeyev SD (1992) A biosensor for L-proline determination by use of
immobilized microbial cells. Appl Biochem Biotechnol 36:199–210
37. Rainina EI, Efremenco EN, Varfolomeyev SD (1996) The development of a new biosensor
based on recombinant E. coli for the direct detection of organophosphorus neurotoxins.
Biosens Bioelectron 11:991–1000
38. Philp JC, Balmand S, Hajto E, Bailey MJ, Wio ¨es S, Whiteley AS, Lilley AK, Hajto J,
Dunbar SA (2003) Whole cell immobilized biosensors for toxicity assessment of a wastewater
treatment plant treating phenolics-containing waste. Anal Chim Acta 487:61–74
39. Ha ¨ggstro ¨m L, Enfors SO (1982) Continuous production of butanol with immobilized cells of
Clostridium acetobutylicum. Appl Biochem Biotechnol 7:35–37
40. Senthuran A, Senthuran V, Hatti-Kaul R, Mattiasson B (1999) Lactic acid production by
immobilized Lactobacillus casei in recycle batch reactor: a step towards optimization.
J Biotechnol 73:61–70
41. Plieva FM, Oknianska A, Degerman E, Mattiasson B (2008) Macroporous gel particles as
robust macroporous matrices for cell immobilization. Biotechnol J 3:410–417
42. Plieva FM, Galaev IY, Mattiasson B (2007) Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate containing fluids and cell culture
applications. J Sep Sci 30:1657–1671
43. Nilsang S, Nehru V, Plieva FM, Nandakumar KS, Rakshit SK, Holmdahl R, Mattiasson B,
Kumar A (2008) Three-dimensional culture for monoclonal antibody production by hybridoma
cells immobilized in macroporous gel particles. Biotechnol Prog 24:1122–1131
44. Kumar A, Rodrigues-Caballero A, Plieva FM, Galaev IY, Nandakumar KS, Kamihira M,
Holmdal R, Orfao A, Mattiasson B (2005) Affinity binding of cells to cryogel adsorbents with
immobilized specific ligands: effect of ligand coupling and matrix architexture. J Mol
Recognit 18:84–93
45. Kirsebom H, Mattiasson B, Galaev IY (2009) Building macroporous material from microgels
and microbes via one-step cryogelation. Langmuir 25:8462–8465
46. Kirsebom H, Mattiasson B (2011) Cryostructuration as a tool for preparing highly porous
polymer materials. Polym Chem 2:1059–1062
47. Zaushitsyna O, Berillo D, Kirsebom H, Mattiasson B (2014) Cryostructured and crosslinked
viable cells forming monoliths suitable for bioreactor applications. Top Catal 57:339–348.
doi:10.1007/s11244-013-0189-9
48. Aragao Bo ¨rner R, Zaushitsyna O, Derillo D, Scaccia N, Mattiasson B, Kirsebom H (2013)
Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through
cryogelation for butanol production. Proc Biochem 49:10–18
49. Elowsson L, Kirsebom H, Carmignac V, Mattiasson B, Durbeej M (2013) Evaluation of
macroporous blood and plasma scaffolds for skeletal muscle tissue engineering. Biomater Sci
4:402–408
50. Yang ST, Luo J, Chen C (2004) A fibrous-bed bioreactor of continuous production of
monoclonal antibody by hybridoma. Adv Biochem Eng Biotechnol 87:61–96
Cryogels for Biotechnological Applications
277
cryogel-immobilized Rhodococcus strains in crude oil-contaminated soil and their impact on
biodegradation efficiency. Int Biodeter Biodegr 84:118–125
33. Cunningham CJ, Ivshina IB, Lozinsky VI, Kuyukina MS, Philp JC (2004) Bioremediation of
diesel-contaminated soil by microorganisms immobilized in polyvinyl alcohol. Int Biodeter
Biodegr 54:167–174
34. Siripattanakul S, Wirojanagud W, McEvoy J, Khan E (2008) Effect of cell-to-matrix ratio in
polyvinyl alcohol immobilized pure and mixed cultures on atrazine degradation. Water Air
Soil Pollut 8:257–266
35. Rainina EI, Badalian IE, Ignatov OV, Fedorov AY, Simonian AL, Varfolomeyev SD (1996)
Cell biosensor for detection of phenol in aqueous solutions. Appl Biochem Biotechnol
56:117–127
36. Simonian AL, Rainina EI, Lozinsky VI, Badalian IE, Khachatrian GE, Tatikian SS,
Makhlis TA, Vorfolomeyev SD (1992) A biosensor for L-proline determination by use of
immobilized microbial cells. Appl Biochem Biotechnol 36:199–210
37. Rainina EI, Efremenco EN, Varfolomeyev SD (1996) The development of a new biosensor
based on recombinant E. coli for the direct detection of organophosphorus neurotoxins.
Biosens Bioelectron 11:991–1000
38. Philp JC, Balmand S, Hajto E, Bailey MJ, Wio ¨es S, Whiteley AS, Lilley AK, Hajto J,
Dunbar SA (2003) Whole cell immobilized biosensors for toxicity assessment of a wastewater
treatment plant treating phenolics-containing waste. Anal Chim Acta 487:61–74
39. Ha ¨ggstro ¨m L, Enfors SO (1982) Continuous production of butanol with immobilized cells of
Clostridium acetobutylicum. Appl Biochem Biotechnol 7:35–37
40. Senthuran A, Senthuran V, Hatti-Kaul R, Mattiasson B (1999) Lactic acid production by
immobilized Lactobacillus casei in recycle batch reactor: a step towards optimization.
J Biotechnol 73:61–70
41. Plieva FM, Oknianska A, Degerman E, Mattiasson B (2008) Macroporous gel particles as
robust macroporous matrices for cell immobilization. Biotechnol J 3:410–417
42. Plieva FM, Galaev IY, Mattiasson B (2007) Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate containing fluids and cell culture
applications. J Sep Sci 30:1657–1671
43. Nilsang S, Nehru V, Plieva FM, Nandakumar KS, Rakshit SK, Holmdahl R, Mattiasson B,
Kumar A (2008) Three-dimensional culture for monoclonal antibody production by hybridoma
cells immobilized in macroporous gel particles. Biotechnol Prog 24:1122–1131
44. Kumar A, Rodrigues-Caballero A, Plieva FM, Galaev IY, Nandakumar KS, Kamihira M,
Holmdal R, Orfao A, Mattiasson B (2005) Affinity binding of cells to cryogel adsorbents with
immobilized specific ligands: effect of ligand coupling and matrix architexture. J Mol
Recognit 18:84–93
45. Kirsebom H, Mattiasson B, Galaev IY (2009) Building macroporous material from microgels
and microbes via one-step cryogelation. Langmuir 25:8462–8465
46. Kirsebom H, Mattiasson B (2011) Cryostructuration as a tool for preparing highly porous
polymer materials. Polym Chem 2:1059–1062
47. Zaushitsyna O, Berillo D, Kirsebom H, Mattiasson B (2014) Cryostructured and crosslinked
viable cells forming monoliths suitable for bioreactor applications. Top Catal 57:339–348.
doi:10.1007/s11244-013-0189-9
48. Aragao Bo ¨rner R, Zaushitsyna O, Derillo D, Scaccia N, Mattiasson B, Kirsebom H (2013)
Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through
cryogelation for butanol production. Proc Biochem 49:10–18
49. Elowsson L, Kirsebom H, Carmignac V, Mattiasson B, Durbeej M (2013) Evaluation of
macroporous blood and plasma scaffolds for skeletal muscle tissue engineering. Biomater Sci
4:402–408
50. Yang ST, Luo J, Chen C (2004) A fibrous-bed bioreactor of continuous production of
monoclonal antibody by hybridoma. Adv Biochem Eng Biotechnol 87:61–96
Cryogels for Biotechnological Applications
277