68. Savina IN, Mattiasson B, Galaev IY (2006) Graft polymerization of vinyl monomers inside
macroporous polyacrylamide gel, cryogel, in aqueous and aqueous-organic media initiated by
diperiodatocuproate(III) complexes. J Polym Sci Polym Chem 44:1952–1963
69. Savina IN, Galaev IY, Mattiasson B (2006) Ion-exchange macroporous hydrophilic gel
monolith with grafted polymer brushes. J Mol Recognit 19:315–321
70. Yao K, Yun J, Shen S, Chen F (2007) In-situ graft-polymerization preparation of cationexchange supermacroporus cryogel with sulfo groups in glass columns. J Chromatogr 1157:
246–251
71. Hanora A, Savina IN, Plieva FM, Izumrudov VA, Mattiasson B, Galaev IY (2006) Direct
capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths. J Biotechnol 123:343–355
72. U ¨ nlu ¨ N, Ceylan S, Erzengin M, Odabasi M (2011) Investigation of protein adsorption
performance of Ni
2+ -attached diatomite particles embedded in composite monolithic
cryogels. J Sept Sci 34:2173–2180
73. Erzengin M, U ¨ nlu ¨ N, Odabasi M (2011) Novel adsorbent for protein chromatography:
Supermacroporous monolithic cryogel embedded with Cu
2+ -attached sporopollenin particles.
J Chromatogr 1218:484–490
74. Xu P, Yao Y, Shen S, Yun J, Yao K (2010) Preparation of supermacroporous composite
cryogel embedded with SiO2 nanoparticles. Biotechnol Bioeng Chin J Chem Eng 18:
667–671
75. Hajizadeh S, Kirsebom H, Leistner A, Mattiasson B (2012) Composite cryogel with
immobilized concanavalin A for affinity chromatography of glycoproteins. J Sept Sci 25:
2978–2985
76. Savina IN, English CJ, Whitby RLD, Zheng Y, Leistner A, Mikhalosky SV, Cundy AB
(2011) High efficiency removal of dissolved As(III) using iron nanoparticle-embedded
macroporous polymer composites. J Hazard Mater 192:1002–1008
77. Sun S, Tang Y, Fu Q, Liu X, Guo L, Zhao Y, Chang C (2012) Monolithic cryogel made of
agarose-chitosan composite and loaded with agarose beads for purification of immunoglobulin
G. Int J Biol Macromol 50:1002–1007
78. Hajizadeh S, Kirsebom H, Mattiasson B (2010) Characterization of macroporous carbonstructured particle gel as adsorbent for small organic molecules. Soft Matter 6:5562–5569
79. Komarova GA, Starodubtsev SG, Lozinsky VI, Kalinina EV, Landfester K, Kokhlov AR
(2008) Intelligent gels and cryogels with entrapped emulsions. Langmuir 24:4467–4469
80. Podorozhko EA, Korlyukov AA, Lozinsky VI (2010) Cryostructuring of polymer systems,
XXX. Poly(vinyl alcohol)-based composite cryogels filled with small disperse oil droplets: a
gel system capable of mechanically induced releasing of lipophilic constituents. J Appl
Polym Sci 117:1332–1349
81. Plieva FM, Ekstro ¨m P, Galaev IY, Mattiasson B (2008) Monolithic cryogels with open
porous structure and unique double-continuous macroporous networks. Soft Matter 4:
2418–2428
82. Akduman B, Uygun M, Uygun DA, Akgo ¨l S, Denizli A (2013) Purification of yeast alcohol
dehydrogenase by using immobilized metal affinity cryogels. Mat Sci Eng C 33:4842–4848
83. Yilmaz F, Bereli N, Yavuz H, Denizli A (2009) Supermacropoorous hydrophobic affinity
cryogels for protein chromatography. Biochem Eng J 43:272–279
84. Demiryas N, Tu ¨zmen N, Galaev IY, Piskin E, Denizli A (2007) Poly(acrylamide-allyl
glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography. J Appl
Polym Sci 105:1808–1816
85. Alkan H, Bereli N, Baysal Z, Denizli A (2009) Antibody purification with protein A attached
to supermacroporous poly(hydroxyethyl methacrylate) cryogel. Biochem Eng J 45:207–208
86. Bakhshpour M, Bereli N, Senel S (2014) Preparation and characterization of thiophilic
cryogels with 2-mercapto ethanol as a ligand for IgG purification. Colloid Surface B 113:
261–268
Cryogels for Biotechnological Applications
279
macroporous polyacrylamide gel, cryogel, in aqueous and aqueous-organic media initiated by
diperiodatocuproate(III) complexes. J Polym Sci Polym Chem 44:1952–1963
69. Savina IN, Galaev IY, Mattiasson B (2006) Ion-exchange macroporous hydrophilic gel
monolith with grafted polymer brushes. J Mol Recognit 19:315–321
70. Yao K, Yun J, Shen S, Chen F (2007) In-situ graft-polymerization preparation of cationexchange supermacroporus cryogel with sulfo groups in glass columns. J Chromatogr 1157:
246–251
71. Hanora A, Savina IN, Plieva FM, Izumrudov VA, Mattiasson B, Galaev IY (2006) Direct
capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths. J Biotechnol 123:343–355
72. U ¨ nlu ¨ N, Ceylan S, Erzengin M, Odabasi M (2011) Investigation of protein adsorption
performance of Ni
2+ -attached diatomite particles embedded in composite monolithic
cryogels. J Sept Sci 34:2173–2180
73. Erzengin M, U ¨ nlu ¨ N, Odabasi M (2011) Novel adsorbent for protein chromatography:
Supermacroporous monolithic cryogel embedded with Cu
2+ -attached sporopollenin particles.
J Chromatogr 1218:484–490
74. Xu P, Yao Y, Shen S, Yun J, Yao K (2010) Preparation of supermacroporous composite
cryogel embedded with SiO2 nanoparticles. Biotechnol Bioeng Chin J Chem Eng 18:
667–671
75. Hajizadeh S, Kirsebom H, Leistner A, Mattiasson B (2012) Composite cryogel with
immobilized concanavalin A for affinity chromatography of glycoproteins. J Sept Sci 25:
2978–2985
76. Savina IN, English CJ, Whitby RLD, Zheng Y, Leistner A, Mikhalosky SV, Cundy AB
(2011) High efficiency removal of dissolved As(III) using iron nanoparticle-embedded
macroporous polymer composites. J Hazard Mater 192:1002–1008
77. Sun S, Tang Y, Fu Q, Liu X, Guo L, Zhao Y, Chang C (2012) Monolithic cryogel made of
agarose-chitosan composite and loaded with agarose beads for purification of immunoglobulin
G. Int J Biol Macromol 50:1002–1007
78. Hajizadeh S, Kirsebom H, Mattiasson B (2010) Characterization of macroporous carbonstructured particle gel as adsorbent for small organic molecules. Soft Matter 6:5562–5569
79. Komarova GA, Starodubtsev SG, Lozinsky VI, Kalinina EV, Landfester K, Kokhlov AR
(2008) Intelligent gels and cryogels with entrapped emulsions. Langmuir 24:4467–4469
80. Podorozhko EA, Korlyukov AA, Lozinsky VI (2010) Cryostructuring of polymer systems,
XXX. Poly(vinyl alcohol)-based composite cryogels filled with small disperse oil droplets: a
gel system capable of mechanically induced releasing of lipophilic constituents. J Appl
Polym Sci 117:1332–1349
81. Plieva FM, Ekstro ¨m P, Galaev IY, Mattiasson B (2008) Monolithic cryogels with open
porous structure and unique double-continuous macroporous networks. Soft Matter 4:
2418–2428
82. Akduman B, Uygun M, Uygun DA, Akgo ¨l S, Denizli A (2013) Purification of yeast alcohol
dehydrogenase by using immobilized metal affinity cryogels. Mat Sci Eng C 33:4842–4848
83. Yilmaz F, Bereli N, Yavuz H, Denizli A (2009) Supermacropoorous hydrophobic affinity
cryogels for protein chromatography. Biochem Eng J 43:272–279
84. Demiryas N, Tu ¨zmen N, Galaev IY, Piskin E, Denizli A (2007) Poly(acrylamide-allyl
glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography. J Appl
Polym Sci 105:1808–1816
85. Alkan H, Bereli N, Baysal Z, Denizli A (2009) Antibody purification with protein A attached
to supermacroporous poly(hydroxyethyl methacrylate) cryogel. Biochem Eng J 45:207–208
86. Bakhshpour M, Bereli N, Senel S (2014) Preparation and characterization of thiophilic
cryogels with 2-mercapto ethanol as a ligand for IgG purification. Colloid Surface B 113:
261–268
Cryogels for Biotechnological Applications
279