Fungal Production of Single Cell Oil
Using Defatted Oilseed Meals
as Feedstock
Ruma Dutta, D. K. Bhattacharyya, and Jayati Bhowal
Abstract In the present study, the production of single cell oil by three soil isolated
strains of Aspergillus sp. was investigated in various basal lipid production media and
by adding defatted oilseed (flaxseed, mustard, and rice bran) meal as supplements.
Microorganisms were identified after screening from rice bran oil industry-drained
soil, and their growth and lipid production rate were investigated in various fermentation media. The organisms were incubated with various oilseed cakes for higher
oleic acid (18:1) and linoleic acid (18:2) contents. Growth and lipid production were
monitored for 10 days, in which the 7th day showed optimum results. An increase in
lipid yield from 24 to 55% was observed when supplemented with defatted oilseed
meals. The maximum lipid content was observed in potato dextrose medium when
the microorganism was supplemented with 3% flaxseed meal for 7 days. Gas chromatography (GC) analysis showed that the major fatty acids produced were palmitic
(16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic
(18:3).
Keywords Single cell oil · Meal supplements · Aspergillus sp. · Oleic acid ·
Linoleic acid
R. Dutta · D. K. Bhattacharyya · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
R. Dutta
e-mail: 1rumadutta@gmail.com
D. K. Bhattacharyya
e-mail: dkb_oiltech@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_6
51
Using Defatted Oilseed Meals
as Feedstock
Ruma Dutta, D. K. Bhattacharyya, and Jayati Bhowal
Abstract In the present study, the production of single cell oil by three soil isolated
strains of Aspergillus sp. was investigated in various basal lipid production media and
by adding defatted oilseed (flaxseed, mustard, and rice bran) meal as supplements.
Microorganisms were identified after screening from rice bran oil industry-drained
soil, and their growth and lipid production rate were investigated in various fermentation media. The organisms were incubated with various oilseed cakes for higher
oleic acid (18:1) and linoleic acid (18:2) contents. Growth and lipid production were
monitored for 10 days, in which the 7th day showed optimum results. An increase in
lipid yield from 24 to 55% was observed when supplemented with defatted oilseed
meals. The maximum lipid content was observed in potato dextrose medium when
the microorganism was supplemented with 3% flaxseed meal for 7 days. Gas chromatography (GC) analysis showed that the major fatty acids produced were palmitic
(16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic
(18:3).
Keywords Single cell oil · Meal supplements · Aspergillus sp. · Oleic acid ·
Linoleic acid
R. Dutta · D. K. Bhattacharyya · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
R. Dutta
e-mail: 1rumadutta@gmail.com
D. K. Bhattacharyya
e-mail: dkb_oiltech@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_6
51