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R. Dutta et al.
1 Introduction
Single cell oils (SCOs) are oils produced from oleaginous microorganisms. Such
microbes have the ability to utilize carbon, nitrogen, and other nutrients present in the
medium and transform them into oils, accumulated into their cell membrane. SCOs
having different fatty acid compositions from plant seed oils and fish oils, which are
valuable for human life (Sakuradani 2010). The microorganisms such as bacteria,
yeasts, molds, and microalgae that accumulate more than 25% of their biomass as
microbial oil are generally termed as oleaginous due to the similarity of their oils
in fatty acid composition to vegetable oil (Bayizit et al. 2014). Recently, published
studies reported that lignocellulose biomass could be bioconverted into lipids by
oleaginous microorganisms (Qiao et al. 2018). Lignocellulosic feedstocks such as
rice straw, husks, wheat straw, sugarcane bagasse, molasses, oil cakes, and corn stover
are some of such well-recognized substrates that have already proved their worth in
industrial-scale production of several contemporary microbial products (Diwan et al.
2018).
The biotechnology industry has also focused on the ability of oleaginous fungi
to convert agricultural waste streams and residues into specialty lipids containing
polyunsaturated fatty acids of nutraceutical and pharmaceutical importance, such
as γ-linolenic acid (Chan et al. 2018). Linoleic acid has effects on mitochondria,
cancer, and aging (Eldor 2018). Although vegetable oils such as virgin olive oil and
safflower oil contain edible oleic acid and linoleic acid, by breeding of an oleaginous
fungus, it is expected to stably supply fermented microbial oils and to develop new
functional lipids using these breeding fungi (Sakamoto et al. 2017).
2 Materials and Methods
2.1 Materials
All chemicals used were analytical grade, purchased from Merck. FAME standard
was purchased from Supelco.
2.2 Collection of Soil
Soil sample was collected from drained waste of rice bran oil industry situated in
Saktigarh, Burdwan and stored at 4 °C under refrigeration immediately until use.
R. Dutta et al.
1 Introduction
Single cell oils (SCOs) are oils produced from oleaginous microorganisms. Such
microbes have the ability to utilize carbon, nitrogen, and other nutrients present in the
medium and transform them into oils, accumulated into their cell membrane. SCOs
having different fatty acid compositions from plant seed oils and fish oils, which are
valuable for human life (Sakuradani 2010). The microorganisms such as bacteria,
yeasts, molds, and microalgae that accumulate more than 25% of their biomass as
microbial oil are generally termed as oleaginous due to the similarity of their oils
in fatty acid composition to vegetable oil (Bayizit et al. 2014). Recently, published
studies reported that lignocellulose biomass could be bioconverted into lipids by
oleaginous microorganisms (Qiao et al. 2018). Lignocellulosic feedstocks such as
rice straw, husks, wheat straw, sugarcane bagasse, molasses, oil cakes, and corn stover
are some of such well-recognized substrates that have already proved their worth in
industrial-scale production of several contemporary microbial products (Diwan et al.
2018).
The biotechnology industry has also focused on the ability of oleaginous fungi
to convert agricultural waste streams and residues into specialty lipids containing
polyunsaturated fatty acids of nutraceutical and pharmaceutical importance, such
as γ-linolenic acid (Chan et al. 2018). Linoleic acid has effects on mitochondria,
cancer, and aging (Eldor 2018). Although vegetable oils such as virgin olive oil and
safflower oil contain edible oleic acid and linoleic acid, by breeding of an oleaginous
fungus, it is expected to stably supply fermented microbial oils and to develop new
functional lipids using these breeding fungi (Sakamoto et al. 2017).
2 Materials and Methods
2.1 Materials
All chemicals used were analytical grade, purchased from Merck. FAME standard
was purchased from Supelco.
2.2 Collection of Soil
Soil sample was collected from drained waste of rice bran oil industry situated in
Saktigarh, Burdwan and stored at 4 °C under refrigeration immediately until use.