356
P. Talukder
Fig. 6 Alterations in MT2 gene expression by semiquantitative method
Fig. 7 Alterations in MT2
gene expression by real-time
PCR
3.4 Amount of Different Phytochelatins (by HPLC)
The results obtained from carrying out HPLC clearly implied that copper treatment
stimulated the biosyntheses of Phytochelatin 2, 3, 4, 5, and 6 in the brinjal plants.
About 800 μM Cu-treated samples showed the highest content of Phytochelatins 2,
4, 5, and 6; Phytochelatin 3, being the exception showed maximum concentration in
500 μM Cu-treated samples (Figs. 8 and 9).
Fig. 8 HPLC chromatogram of phytochelation
P. Talukder
Fig. 6 Alterations in MT2 gene expression by semiquantitative method
Fig. 7 Alterations in MT2
gene expression by real-time
PCR
3.4 Amount of Different Phytochelatins (by HPLC)
The results obtained from carrying out HPLC clearly implied that copper treatment
stimulated the biosyntheses of Phytochelatin 2, 3, 4, 5, and 6 in the brinjal plants.
About 800 μM Cu-treated samples showed the highest content of Phytochelatins 2,
4, 5, and 6; Phytochelatin 3, being the exception showed maximum concentration in
500 μM Cu-treated samples (Figs. 8 and 9).
Fig. 8 HPLC chromatogram of phytochelation