Role of Metallothionein and Phytochelatin in Combating Abiotic …
355
Fig. 4 Change in total
antioxidant activity
Fig. 5 Change in DPPH
scavenging activity
3.2.2 DPPH Scavenging Activity
There has been a significant increase (about threefold) in the percentage DPPH radical
scavenging activity in the Cu-treated plant extracts (Fig. 5).
3.3 Gene Expression Study by Reverse Transcription
and Real-Time PCR, Respectively
MT2 is known to be expressed predominantly in shoot tissues. It is found that the
expression of MT2 has increased in Cu-treated plants with respect to untreated
control. The densitometric analysis shows 1.36 and 1.38 fold increases in expressions
of 500 and 800 μM Cu-treated samples, respectively, whereas a 1.66 fold increase
in 1000 μM treated sample in comparison to untreated control (Fig. 6).
Real-time PCR of MT2 gene was carried out by 2
− C T method and observed 4.57
and 5.78 fold increases in the expressions of 500 and 800 μM Cu-treated samples,
respectively, compared with untreated control. As we further increased the dosage to
1000 μM, we observed a drop (only 5.64 fold increase) in the relative gene expression
of MT2. β-actin was taken as endogenous control (Fig. 7).
355
Fig. 4 Change in total
antioxidant activity
Fig. 5 Change in DPPH
scavenging activity
3.2.2 DPPH Scavenging Activity
There has been a significant increase (about threefold) in the percentage DPPH radical
scavenging activity in the Cu-treated plant extracts (Fig. 5).
3.3 Gene Expression Study by Reverse Transcription
and Real-Time PCR, Respectively
MT2 is known to be expressed predominantly in shoot tissues. It is found that the
expression of MT2 has increased in Cu-treated plants with respect to untreated
control. The densitometric analysis shows 1.36 and 1.38 fold increases in expressions
of 500 and 800 μM Cu-treated samples, respectively, whereas a 1.66 fold increase
in 1000 μM treated sample in comparison to untreated control (Fig. 6).
Real-time PCR of MT2 gene was carried out by 2
− C T method and observed 4.57
and 5.78 fold increases in the expressions of 500 and 800 μM Cu-treated samples,
respectively, compared with untreated control. As we further increased the dosage to
1000 μM, we observed a drop (only 5.64 fold increase) in the relative gene expression
of MT2. β-actin was taken as endogenous control (Fig. 7).