Application of Bacillus sp. NITD 19
for Utilization of Cyanide as Nutrient
Source
Abhilasha Rai, Sanket Mukherjee, Agradeep Mukherjee,
Jitamanyu Chakrabarty, Pinaki Bhattacharya, and Susmita Dutta
Abstract Cyanide is highly toxic to our ecosystem. The main contributor of cyanide
to the environment is the untreated or improperly treated wastewater coming from
various industries like steel, metallurgical, mining, tanning, etc. Bioremediation is
an inexpensive yet effective measure, compared to physico-chemical methods, for
removal of cyanide from industrial effluents. In the present study, one indigenous
bacterial strain was collected and isolated from the outfall of nearby coke-oven
plant and identified as Bacillus sp. NITD 19 through 16S rRNA gene sequencing.
The test strain was used for removal of cyanide from synthetic solution. Lethal
dose analysis was performed and the strain was found to grow up to 100 mg/L
initial cyanide concentration. One factor at a time (OFAT) analysis was performed
to get the effective operational parameters for maximum removal by varying pH
(6–10), inoculum load (1–4%), temperature (25–37
o C), and initial concentration
A. Rai · S. Dutta (B)
Department of Chemical Engineering, National Institute of Technology Durgapur, Mahatma
Gandhi Avenue, Durgapur, West Bengal 713209, India
e-mail: susmita.dutta@che.nitdgp.ac.in
A. Rai
e-mail: abhilasharai.rai@gmail.com
S. Mukherjee · A. Mukherjee
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata 700107,
India
e-mail: smukherjee109@gmail.com
A. Mukherjee
e-mail: agra4mukherjee@gmail.com
J. Chakrabarty
Department of Chemistry, National Institute of Technology Durgapur, Mahatma Gandhi Avenue,
Durgapur, West Bengal 713209, India
e-mail: jitamanyu.chakrabarty@ch.nitdgp.ac.in
P. Bhattacharya
Department of Chemical Engineering, Heritage Institute of Technology, Anandapur, Kolkata
700107, India
e-mail: pinaki.bhattacharya@heritageit.edu
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_24
241
for Utilization of Cyanide as Nutrient
Source
Abhilasha Rai, Sanket Mukherjee, Agradeep Mukherjee,
Jitamanyu Chakrabarty, Pinaki Bhattacharya, and Susmita Dutta
Abstract Cyanide is highly toxic to our ecosystem. The main contributor of cyanide
to the environment is the untreated or improperly treated wastewater coming from
various industries like steel, metallurgical, mining, tanning, etc. Bioremediation is
an inexpensive yet effective measure, compared to physico-chemical methods, for
removal of cyanide from industrial effluents. In the present study, one indigenous
bacterial strain was collected and isolated from the outfall of nearby coke-oven
plant and identified as Bacillus sp. NITD 19 through 16S rRNA gene sequencing.
The test strain was used for removal of cyanide from synthetic solution. Lethal
dose analysis was performed and the strain was found to grow up to 100 mg/L
initial cyanide concentration. One factor at a time (OFAT) analysis was performed
to get the effective operational parameters for maximum removal by varying pH
(6–10), inoculum load (1–4%), temperature (25–37
o C), and initial concentration
A. Rai · S. Dutta (B)
Department of Chemical Engineering, National Institute of Technology Durgapur, Mahatma
Gandhi Avenue, Durgapur, West Bengal 713209, India
e-mail: susmita.dutta@che.nitdgp.ac.in
A. Rai
e-mail: abhilasharai.rai@gmail.com
S. Mukherjee · A. Mukherjee
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata 700107,
India
e-mail: smukherjee109@gmail.com
A. Mukherjee
e-mail: agra4mukherjee@gmail.com
J. Chakrabarty
Department of Chemistry, National Institute of Technology Durgapur, Mahatma Gandhi Avenue,
Durgapur, West Bengal 713209, India
e-mail: jitamanyu.chakrabarty@ch.nitdgp.ac.in
P. Bhattacharya
Department of Chemical Engineering, Heritage Institute of Technology, Anandapur, Kolkata
700107, India
e-mail: pinaki.bhattacharya@heritageit.edu
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_24
241