Effect of Metabolic Risk Factors, Gene
Polymorphisms and Family History
Among T2DM Population in Asian
Indians
Plaban Chaudhuri, Riju Ghosh, Mithun Das, Indrani Lodh,
and Riddhi Goswami
Abstract Women with a family history of diabetes (FHD) are at significantly
increased risk of developing gestational diabetes mellitus, which is itself an important risk factor of childhood obesity and type 2 diabetes mellitus (T2DM) in early
age. Elevated C-reactive protein (CRP) is a marker of low-grade systemic inflammation and involved in the etiology of diabetes. Insulin receptor substrate (IRS)
molecules are key mediators in insulin signaling and development of diabetes. The
present study was conducted on 100 healthy (non-diabetic, normotensive) adult Asian
Indian women, including 50 with and 50 without FHD, living in and around Kolkata,
India. During the gestation period they were studied twice, and followed up till
delivery. During delivery both mothers’ venous blood and cord blood were collected
to estimate serum CRP, glucose, and lipid profiles of the respective mothers and their
newborns. Genotyping of IRS-1, IRS-2 and CRP polymorphisms was done from these
blood samples. A comparison of the metabolic variables among the subjects with
and without FHD revealed significant differences among them. We also found close
association of several polymorphisms in case of all three genes for both mothers and
their newborn babies. More specifically, genotyping results for mothers with FHD
and their newborn babies show susceptibility to T2DM: (i) for IRS-1 via diseased A
allele (57%) which is carried over to the newborn babies (43%), (ii) for IRS-2 via
P. Chaudhuri · R. Ghosh · R. Goswami (B)
Department of Biotechnology, Heritage Institute of Technology, Kolkata, India
e-mail: riddhi.goswami@heritageit.edu
P. Chaudhuri
e-mail: plaban.chaudhuri@heritageit.edu
R. Ghosh
e-mail: rijughosh1992@gmail.com
M. Das
Department of Anthropology & Tribal Studies, Sidho-Kanho-Birsha University, Purulia, India
e-mail: mithundas01@yahoo.com
I. Lodh
Urvaraa IVF Clinic, Kolkata, India
e-mail: drindranilodh@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_14
143
Polymorphisms and Family History
Among T2DM Population in Asian
Indians
Plaban Chaudhuri, Riju Ghosh, Mithun Das, Indrani Lodh,
and Riddhi Goswami
Abstract Women with a family history of diabetes (FHD) are at significantly
increased risk of developing gestational diabetes mellitus, which is itself an important risk factor of childhood obesity and type 2 diabetes mellitus (T2DM) in early
age. Elevated C-reactive protein (CRP) is a marker of low-grade systemic inflammation and involved in the etiology of diabetes. Insulin receptor substrate (IRS)
molecules are key mediators in insulin signaling and development of diabetes. The
present study was conducted on 100 healthy (non-diabetic, normotensive) adult Asian
Indian women, including 50 with and 50 without FHD, living in and around Kolkata,
India. During the gestation period they were studied twice, and followed up till
delivery. During delivery both mothers’ venous blood and cord blood were collected
to estimate serum CRP, glucose, and lipid profiles of the respective mothers and their
newborns. Genotyping of IRS-1, IRS-2 and CRP polymorphisms was done from these
blood samples. A comparison of the metabolic variables among the subjects with
and without FHD revealed significant differences among them. We also found close
association of several polymorphisms in case of all three genes for both mothers and
their newborn babies. More specifically, genotyping results for mothers with FHD
and their newborn babies show susceptibility to T2DM: (i) for IRS-1 via diseased A
allele (57%) which is carried over to the newborn babies (43%), (ii) for IRS-2 via
P. Chaudhuri · R. Ghosh · R. Goswami (B)
Department of Biotechnology, Heritage Institute of Technology, Kolkata, India
e-mail: riddhi.goswami@heritageit.edu
P. Chaudhuri
e-mail: plaban.chaudhuri@heritageit.edu
R. Ghosh
e-mail: rijughosh1992@gmail.com
M. Das
Department of Anthropology & Tribal Studies, Sidho-Kanho-Birsha University, Purulia, India
e-mail: mithundas01@yahoo.com
I. Lodh
Urvaraa IVF Clinic, Kolkata, India
e-mail: drindranilodh@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_14
143