140
S. Sengupta J. Bhowal
Fig. 1 Peaks of principal antioxidant compounds in pyocyanin by HPLC
Fig. 2 Effect of pyocyanin
on MG-63 osteosarcoma cell
100
100
100
85.57
14.19
11.01
0
50
100
150
Day 1
Day 3
Day 5
Cell viability (%)
Number of days
Control
Pyocyanin
3.6 In Vitro Cytotoxic Effect of Pyocyanin on MG-63
Osteosarcoma Cell Lines
The gradual decline in cell viability percentage established the non-cytotoxic
behavior of pyocyanin toward MG-63 osteosarcoma cell lines with respect to control
(Fig. 2). On day 1, the cell viability percentage was recorded to be 85.57% and
gradually decreased to 11.01% at the end of day 5. Moayedi et al. (2018) reported
that pyocyanin was able to reduce human pancreatic cancer (Panc-1) cells, inhibition
rate being by 89.88 ± 1.86%. He further added that pyocyanin could also induce
dose-dependent apoptosis in Panc-1 cells after 24 h.
4 Conclusion
In vitro investigations showed that pyocyanin exhibits promising antioxidant capacity
to scavenge free radicals in biological system. The pigment thus can be used as an
alternative natural antioxidant after toxicological examination. This high percentage
of free radical scavenging activity of the pigment at very minute concentrations gives
us a positive indication for the safe use of the pigment. In addition to this, the pigment
showed no cytotoxic effects on cultured MG-63 osteosarcoma cell lines.
Acknowledgements The authors express their sincere thanks to Indian Institute of Chemical
Biology (IICB), Kolkata and Centre for Healthcare Science and Technology, IIEST, Shibpur.
S. Sengupta J. Bhowal
Fig. 1 Peaks of principal antioxidant compounds in pyocyanin by HPLC
Fig. 2 Effect of pyocyanin
on MG-63 osteosarcoma cell
100
100
100
85.57
14.19
11.01
0
50
100
150
Day 1
Day 3
Day 5
Cell viability (%)
Number of days
Control
Pyocyanin
3.6 In Vitro Cytotoxic Effect of Pyocyanin on MG-63
Osteosarcoma Cell Lines
The gradual decline in cell viability percentage established the non-cytotoxic
behavior of pyocyanin toward MG-63 osteosarcoma cell lines with respect to control
(Fig. 2). On day 1, the cell viability percentage was recorded to be 85.57% and
gradually decreased to 11.01% at the end of day 5. Moayedi et al. (2018) reported
that pyocyanin was able to reduce human pancreatic cancer (Panc-1) cells, inhibition
rate being by 89.88 ± 1.86%. He further added that pyocyanin could also induce
dose-dependent apoptosis in Panc-1 cells after 24 h.
4 Conclusion
In vitro investigations showed that pyocyanin exhibits promising antioxidant capacity
to scavenge free radicals in biological system. The pigment thus can be used as an
alternative natural antioxidant after toxicological examination. This high percentage
of free radical scavenging activity of the pigment at very minute concentrations gives
us a positive indication for the safe use of the pigment. In addition to this, the pigment
showed no cytotoxic effects on cultured MG-63 osteosarcoma cell lines.
Acknowledgements The authors express their sincere thanks to Indian Institute of Chemical
Biology (IICB), Kolkata and Centre for Healthcare Science and Technology, IIEST, Shibpur.