Study on the Antioxidant and Cytotoxic Properties …
139
3.4.4 Reducing Power Assay (RPA)
RPA (mEq BHT/gm) of the pigment was found to be 34.8 ± 0.00. BHT was used as
the standard. Pawar et al. (2015) tested the reducing power activity with the pigment
produced by P. koreensis. The highest activity was recorded at 15 mg/ml.
3.4.5 Metal Chelating Activity (MCA)
MCA (%) of the pigment was found to be 20.78 ± 0.02 at 1.36 mg/ml and it represents
the greatest inhibition activity when compared to the standard, EDTA. Mani et al.
(2015) observed in their study that FC1-3 showed the highest metal chelating activity
at 1.53 mg/ml.
3.4.6 Total Phenolic Content (TPC)
TPC was found to be 26.47 ± 0.9 mg GAE/ml. Gallic acid was served as standard.
In the study conducted by Mullick et al. (2015), it was reported that the TPC of the
pigment extracted from P. aeruginosa MTCC 741 was 23.53 ± 2.5 mg GAE/ml.
3.4.7 Total Flavonoid Content (TFC)
TFC was found to be 32 ± 0.82 mg CAE/ml which is higher than in the study
conducted by Mullicket al. (2015) who reported that the TFC of the pigment extracted
from P. aeruginosa MTCC 741 was 30 ± 0.99 mg QE/ml.
3.5 Identification of Antioxidant Compounds Present
in the Pigment Extract by High-Power Liquid
Chromatography (HPLC) Analysis
The phenolic and flavonoid profiling of the pigment extract was identified by HPLC
coupled to photodiode array detector (Fig. 1). Aspartic acid, gallic acid, dihydroxy
benzoic acid (DHBA), trans cinnamic acid, and ferulic acid were identified as the principal antioxidant compounds present in the pigment extract. This has been reported
for the first time.
139
3.4.4 Reducing Power Assay (RPA)
RPA (mEq BHT/gm) of the pigment was found to be 34.8 ± 0.00. BHT was used as
the standard. Pawar et al. (2015) tested the reducing power activity with the pigment
produced by P. koreensis. The highest activity was recorded at 15 mg/ml.
3.4.5 Metal Chelating Activity (MCA)
MCA (%) of the pigment was found to be 20.78 ± 0.02 at 1.36 mg/ml and it represents
the greatest inhibition activity when compared to the standard, EDTA. Mani et al.
(2015) observed in their study that FC1-3 showed the highest metal chelating activity
at 1.53 mg/ml.
3.4.6 Total Phenolic Content (TPC)
TPC was found to be 26.47 ± 0.9 mg GAE/ml. Gallic acid was served as standard.
In the study conducted by Mullick et al. (2015), it was reported that the TPC of the
pigment extracted from P. aeruginosa MTCC 741 was 23.53 ± 2.5 mg GAE/ml.
3.4.7 Total Flavonoid Content (TFC)
TFC was found to be 32 ± 0.82 mg CAE/ml which is higher than in the study
conducted by Mullicket al. (2015) who reported that the TFC of the pigment extracted
from P. aeruginosa MTCC 741 was 30 ± 0.99 mg QE/ml.
3.5 Identification of Antioxidant Compounds Present
in the Pigment Extract by High-Power Liquid
Chromatography (HPLC) Analysis
The phenolic and flavonoid profiling of the pigment extract was identified by HPLC
coupled to photodiode array detector (Fig. 1). Aspartic acid, gallic acid, dihydroxy
benzoic acid (DHBA), trans cinnamic acid, and ferulic acid were identified as the principal antioxidant compounds present in the pigment extract. This has been reported
for the first time.