134
S. Sengupta J. Bhowal
The current study aims at the exploitation of different antioxidant activities
of pyocyanin, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity, 2,2
-azino-bis-3ethylbenzthiazoline-6-sulphonic acid (ABTS) activity, reducing power assay (RPA),
metal chelating activity, and total phenolic content (TPC) and total flavonoid content
(TFC). In vitro cytotoxic effects of pyocyaninon osteosarcoma cell line MG-63 was
also studied. An in-depth study on the various antioxidant activities of pyocyanin has
been done. We reported for the first time, cytotoxic effect of pyocyanin on MG-63
osteosarcoma cell line.
2 Materials and Methods
2.1 Chemicals
Analytical grade chemicals and solvents were obtained from Sigma Aldrich, Sisco
Research Laboratories Pvt. Ltd. (SRL) and Merck, India. Standards like ascorbic
acid, ethylene diamine tetra acetic acid (EDTA), beta hydroxy toluene (BHT), gallic
acid, and catechin were procured from Sigma Aldrich. MG-63 osteosarcoma cell
lines were procured from National Centre for Cell Science (NCCS), Pune, India.
2.2 Screening of Pigment-Producing Microorganism
Screening of the pigment-producing microorganism from soil was previously
performed by serial dilution and spread plate method on nutrient agar plates and
incubated at 37 °C for 18–24 h. This study had earlier been reported in our previous
publication, Chatterjee and Bhowal (2016).
2.3 Identification of the Pigment-Producing Microorganism
Standard biochemical tests and molecular characterization of the microorganism
were done for the identification purpose (El-Fouly et al. 2015).
S. Sengupta J. Bhowal
The current study aims at the exploitation of different antioxidant activities
of pyocyanin, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric-reducing antioxidant power (FRAP) activity, 2,2
-azino-bis-3ethylbenzthiazoline-6-sulphonic acid (ABTS) activity, reducing power assay (RPA),
metal chelating activity, and total phenolic content (TPC) and total flavonoid content
(TFC). In vitro cytotoxic effects of pyocyaninon osteosarcoma cell line MG-63 was
also studied. An in-depth study on the various antioxidant activities of pyocyanin has
been done. We reported for the first time, cytotoxic effect of pyocyanin on MG-63
osteosarcoma cell line.
2 Materials and Methods
2.1 Chemicals
Analytical grade chemicals and solvents were obtained from Sigma Aldrich, Sisco
Research Laboratories Pvt. Ltd. (SRL) and Merck, India. Standards like ascorbic
acid, ethylene diamine tetra acetic acid (EDTA), beta hydroxy toluene (BHT), gallic
acid, and catechin were procured from Sigma Aldrich. MG-63 osteosarcoma cell
lines were procured from National Centre for Cell Science (NCCS), Pune, India.
2.2 Screening of Pigment-Producing Microorganism
Screening of the pigment-producing microorganism from soil was previously
performed by serial dilution and spread plate method on nutrient agar plates and
incubated at 37 °C for 18–24 h. This study had earlier been reported in our previous
publication, Chatterjee and Bhowal (2016).
2.3 Identification of the Pigment-Producing Microorganism
Standard biochemical tests and molecular characterization of the microorganism
were done for the identification purpose (El-Fouly et al. 2015).