Study on the Antioxidant and Cytotoxic
Properties of Pyocyanin Extracted
from Pseudomonas aeruginosa
Sucharita Sengupta and Jayati Bhowal
Abstract Microbial pigments are a promising alternative source for natural
pigments. They possess a great potential for application due to their natural color,
safety, and low production cost. The present investigation is carried out on the
antioxidant assays, namely, 2,2-di phenyl-1 picryl-hydrazyl (DPPH), 2,2
-azinobis3-ehtylbenzthiazoline-6-sulfonate (ABTS), metal chelating activity, reducing power,
and ferric reducing antioxidant power (FRAP) of pyocyanin produced from Pseudomonas aeruginosa. Principal antioxidant compounds present in pyocyanin were
identified by HPLC. In vitro cytotoxicity study was carried out to evaluate the effect
of pyocyanin on MG-63 osteosarcoma cell line.
Keywords Pseudomonas aeruginosa · Pyocyanin · Antioxidant activity · MTT
assay · HPLC
1 Introduction
Microbes have been exploited for pigment production due to factors like relatively
large and easily manipulated strands of genes, independent of weather conditions,
etc. (Shaikh 2016). The increasing awareness of health and pollution hazards of
chemical dye has led to the resurgence of interest in microbial color (Kant 2012).
The most characteristic feature of Pseudomonas aeruginosa is the production of a
water-soluble blue-green phenazine compound known as pyocyanin (Sudhakar et al.
2013).
S. Sengupta · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, P.O. Botanic Garden, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
S. Sengupta
e-mail: sucharitac66@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_13
133
Properties of Pyocyanin Extracted
from Pseudomonas aeruginosa
Sucharita Sengupta and Jayati Bhowal
Abstract Microbial pigments are a promising alternative source for natural
pigments. They possess a great potential for application due to their natural color,
safety, and low production cost. The present investigation is carried out on the
antioxidant assays, namely, 2,2-di phenyl-1 picryl-hydrazyl (DPPH), 2,2
-azinobis3-ehtylbenzthiazoline-6-sulfonate (ABTS), metal chelating activity, reducing power,
and ferric reducing antioxidant power (FRAP) of pyocyanin produced from Pseudomonas aeruginosa. Principal antioxidant compounds present in pyocyanin were
identified by HPLC. In vitro cytotoxicity study was carried out to evaluate the effect
of pyocyanin on MG-63 osteosarcoma cell line.
Keywords Pseudomonas aeruginosa · Pyocyanin · Antioxidant activity · MTT
assay · HPLC
1 Introduction
Microbes have been exploited for pigment production due to factors like relatively
large and easily manipulated strands of genes, independent of weather conditions,
etc. (Shaikh 2016). The increasing awareness of health and pollution hazards of
chemical dye has led to the resurgence of interest in microbial color (Kant 2012).
The most characteristic feature of Pseudomonas aeruginosa is the production of a
water-soluble blue-green phenazine compound known as pyocyanin (Sudhakar et al.
2013).
S. Sengupta · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, P.O. Botanic Garden, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
S. Sengupta
e-mail: sucharitac66@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_13
133